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Reaction Biology/ATAD2B (His)/RD-11-207

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RD-11-207

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Reaction Biology

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DESCRIPTION:Human recombinant ATAD2B bromodomain. In addition to a bromodomain, native ATAD2B contains an AAA ATPase domain. Expressed and located to the nucleus during neuronal development (chick embryos), ATAD2B is expressed at elevated levels in some human tumors (glioblastoma, oligodendroglioma, breast cancer), but is present in the cytoplasm or membrane-bound fractions. The ATAD2B bromodomain binds various Lys(Ac) residues in singly acetylated histone peptide microarrays (histones H1.4, H2A, H2B, H3, H4) with binding to histone H4 K5(Ac) confirmed in solution by isothermal titration calorimetry (ITC). ACCESSION #: NM_017552Uniprot LinkINCLUDES AMINO ACIDS: 952-1086TAG(S): N-terminal His-tagMW: 18.3 kDaEXPRESSION SYSTEM: E. coliSUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris-HCl pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v)STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.View Product Data Sheet Substantial Discounts Available on Bulk QuantitiesWe also offer assay services for ATAD2B (His).

Reaction Biology描述：

人类重组EZH2incomplexwithhumanrecombinantsEED.TotalcomplexMWis228.7kDa.Allproteinsarefull长度（residue2throughC末端）也是全美-expressedinaninsectcell / baculovirusexpressionsystem.TheEEDsubunitincorporatesanN-terminalStrepandFlag-tagandallothersincludeanN-terminalHis-tag.CatalyzesthetransferofmethylgroupsfromS腺苷-L-甲硫氨酸（SAM）tothee-aminofunctionofproteinL-lysineresidues，specificallylysine-27ofhistoneH3（ H3K27）。在开发过程中，Polycomb Repressive Complex 2（PRC2）是主要的甲基转移酶对IBL的反应eforgeneratingtrimethylatedhistoneH3lysine-27（的H3K27me3），anepigeneticmarkessentialforprogrammedrepressionofgeneexpression1-5.EZH2，whichincludesaSETmethyltransferasedomain，isthecatalyticsubunitofPRC21,6.ThecoreofthecatalyticcomplexincludesEZH2，EED，SUZ12andRbAp48，whileadditionofAEBP2significantlyenhancesthemethyltransferaseactivityofthecomplex（> 3×）6.EZH2isoverexpressedinawiderangeofhumancancersanditsoverexpressioncancorrelatewithtumorprogression，increasedmetastasisandpoorprognosis（seereview7）.DepletionofEZH2and / orotherPRC2componentscaninhibitgrowthorinduceapoptosisincancercells6,8-10.Consequently，EZH2isconsideredapromisingtargetforthedevelopmentofanti -癌症疗法11

存取编号： EZH2NM_001203247，EEDNM_003797，SUZ12NM_015355

包括酰胺酸：EZH22-746，EED2-441，SUZ122-739

标记（S）：EEDhasanN末端链霉菌标记标签，其他所有N末端His标签

分子量：228.7kDa

EXPRESSIONSYSTEM ：昆虫细胞/杆状病毒

SUPPLIEDAS：Solutionofpurifiedrecombinantproteinin20mMTris盐酸，pH8,150mMNaCl，2mMMgCl 2，2mMDTT，20％甘油（w / v），0.01％NP-40asdeterminedbyOD 280。

储存：-70°C。在使用前应迅速融化并储存。应保留未使用的未稀释蛋白质，例如干燥/冰乙醇液中的氮。尽可能减少冻融/解冻，但不建议使用稀释体积太小（<5µl）的稀释蛋白质。