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Nordicmubio/Laminin, human/LA24811

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Laminin, humanCatalogue number: LA24811Product Type Polyclonal AntibodyPrimary AntibodiesUnits0.5 ml HostRabbit Species reactivity HumanApplication ELISAImmunofluoresenceImmunohistochemistry (paraffin)Radioimmunoassay BackgroundThe antibody against Laminin primarily labels basal membranes (especially s. c. lamina lucida) and may be used to detect disturbances in the basal membrane during tumour invasion. Laminin is also found in extracellular matrix not related to basal membranes, e.g. in the proximity of neurons especially during development, regeneration and tumour development. Laminins are glycoproteins of the extracellular matrix, especially of the basal membranes. They are multi-functional molecules which play an important role during development and differentiation of organs and for cell movements. These properties rely on the interaction of laminins with receptors on cell surfaces like integrins, collagen IV, proteoglycans. Laminins consist of 2 heavy chains (A and M, 300-400 kDa) and 1 light chain (B1, B2 or S, 200 - 220 kDa).Human laminin fragment P1 100%, human collagen type I and IV Source Immunogen: Purified laminin P1 fragment from human placenta ProductAffinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added! Purification Method: Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!Concentration: app. 1 mg/ml Secondary Reagents: Anti-rabbit IgG-conjugates SpecificitySpecies Reactivity: Human ApplicationsIFA, IHC(P), ELISA, RIA Incubation Time: IHC(P) 60 min at RT or 2-8°C over nightWorking Concentration: (purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:250, ELISA ? 1:200 (OD ? 500)Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Positive Control: Human skin Storage-20°C CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information. ReferencesSee list of references in BIOLOGO ECM brochure. Laminin on human skin paraffin section. LA24811, DAB, hematoxylin

Noridic-Mubio是荷兰的一家著名的免疫学试剂生产厂商，因收购An Der Grub Bio Research GmbH(ADG)，增加了自己的产品线：全球最好的固定破膜剂FIX&PERM 。Nordic-Mubio严格遵照ISO9001标准生产高质量的抗体，主要为针对细胞粘附蛋白、核蛋白和骨架蛋白的抗体。同时Nordic-Mubio也是斑马鱼抗体开发的引领者之一，拥有50多种可用于斑马鱼免疫荧光检测的抗体。