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Kapa biosystems/KAPA hgDNA Quantification and QC Kit/KK4962/300 x 20 µL reactions

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KK4962

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Description:qPCRMasterMix(Bio-Rad®)KAPAhgDNAQuantificationandQCKitQualitymatters.KAPAhgDNAQuantificationandQCKitscontainallthereagentsneededfortheqPCR-basedquantificationandqualityassessmentofhumangenomicDNAsamplespriortoNGSlibraryconstruction.KitscontainKAPASYBR®FASTqPCRMasterMix,optimizedforhigh-performanceSYBRGreenI-basedqPCR,aswellasapre-dilutedsetofDNAstandardsandprimerpremixestargetingdifferentportionsofahighlyconservedsingle-copyhumanlocus.Absolutequantificationisachievedwiththeprimerpairdefiningtheshortestfragment,whereastheadditionalprimersareusedtoderiveinformationabouttheamountofamplifiabletemplateintheDNAsample.Qualityscores(orQ-ratios)generatedwiththekitmaybeusedtopredicttheoutcomeoflibraryconstruction,ortailorworkflowsforsamplesofvariablequality,particularlyFFPEDNA.ThemethodiseasytoautomateandcanbeappliedtoanyprocessorworkflowthatrequiresaccuratequantificationofdiluteDNAsamples,orsamplesthatmaycontainahighproportionofDNAthatisrecalcitranttoPCRamplification.Downloadour KAPAhgDNAQuantificationandQCDataAnalysisTemplate.ForResearchUseOnly.Notforuseindiagnosticprocedures.ProductHighlightsObtainconcentrationandqualityinformationwithasingleassayAllowsforaccuratequantificationofdiluteDNAsamplesQuantificationwithanadditionalprimerpairprovidesaQ-ratiothatisindicativeofsamplequalityEmployqualityscores intheanalysisofNGSlibraryconstructionworkflowsQ-ratioscanprovidevaluableinsightsintothebottlenecksinNGSlibraryconstructionworkflowsForFFPEsamples,libraryandsequencequalityisprimarilylimitedbyinefficientconversionofinputDNAtoadapter-ligatedlibrary0.4yieldedlibrariesthatmetminimumsequencequalityrequirements.Dataonfile.">0.4yieldedlibrariesthatmetminimumsequencequalityrequirements.Dataonfile.">PrevNextObtainactionabledataforsamplepreparationQ-ratioscorrelatewithkeysequencingmetricssuchasduplicationratesandmeantargetcoverageFFPEsampleswithaQscore>0.4yieldlibrariesofacceptablequalitywhenprocessedinstandardsamplepreparationsfortargetcaptureApplications:ApplicationsFFPEsamplesFree-circulatingDNAfromplasmaorserumSamplesobtainedbylaser-capturemicrodissectionoffresh,frozenorFFPEtissueForensicsamplesCellscollectedbyflowcytometryAnyotherlimitingorpreciousclinicalsampleKitSpecificationsandContents/Storage:KitSpecificationsandContents/StorageKitscanbestoredforupto12months at-20˚C.Complete(MasterMix)kitsincludeKAPASYBRFASTqPCRMasterMix(2X),PrimerPremix(41bp,129bpand305bp,10X)andasetof5DNAStandards.PrimerPremixesandDNAStandardsarealsosoldseparately.ComponentsSpecificationsSpecDescriptionCompatIBLePlatformAllNGSplatformsCompatibleSamplesHumangenomicDNASamplesourcesFFPEtissueCellscollectedbylaser-capturemicrodissectionFlow-sortedcellsFree-circulatingDNAfromplasmaorserumForensicsamplesClinicalsamplesStandardcurveconcentrationrange2.5ng/µL–10pg/µLor3,080–12copiesSequencingApplicationsWholeGenomeSequencingWholeExomeSequencingTargetedSequencing(custompanels)AmpliconSequencing

Kapa biosystems成立于2006年，其生产基地位于南非开普敦。致力于研发、生产下一代PCR（Next-Generation PCR）所需的生物酶。分子进化技术（酶工程）打破了现有的研究局限，为生物研究开创了一片新天地。公司技术团队在蛋白质工程、基因组学、蛋白质组学、合成生物学方面获得非常突出的成绩。Kapa biosystems公司追求科学研究与市场化运作的完美结合，执着于“科技创造完美产品，助力生命科学研究”这一最高宗旨。

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