首页 > Kapa biosystems > Kapa biosystems/KAPA Library Amplification Kits/KK2621/250 x 50 µL reactions

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Kapa biosystems/KAPA Library Amplification Kits/KK2621/250 x 50 µL reactions

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KK2621

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Kapa biosystems

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4000-520-616

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Description:WithprimermixKAPALibraryAmplificationKitsThegoldstandardforNGSlibraryamplification.KAPALibraryAmplificationKitscontainKAPAHiFiDNAPolymerase,anovelenzymeengineeredforultra-highfidelityandrobustnessusingKapa’sdirectedevolutiontechnologyplatform.KAPAHiFihasbecometheenzymeofchoiceforNGSlibraryamplificationduetoitsABIlitytoamplifycomplexDNApopulationswithhighfidelity,highefficiency,decreasedPCRduplicationratesandverylowbias.*ThisresultsinlowerduplicationratesandimprovedcoverageofGC-andATrichregions,promoters,lowcomplexityandotherchallengingregionsinallNGSlibraryconstructionworkflowsrequiringlibraryamplification.Inadditiontothestandardlibraryamplificationformulation,KAPAHiFiisavailableinauniquereal-timeformulation,forapplicationsthatdemandprecisecontroloverlibraryamplification.Auracil-tolerantvariant,KAPAHiFiUracil+isalsoavailableforthehigh-efficiency,high-fidelity,low-biasamplificationoflibrariesconstructedfrombisulfite-treatedDNA.KAPALibraryAmplificationKitsforIllumina® platformsnowalsoincludeanoptimallyformulatedLibraryAmplificationPrimerMix.*Dataonfile.ForResearchUseOnly.Notforuseindiagnosticprocedures.ProductHighlightsPrevNextAchievethelowestamplificationbiasandduplicationratesLoweramplificationbiasresultinimprovedrepresentationofalllibraryfragmentsandsequenceregionsHigh-efficiencyamplificationleadstofeweramplificationcyclesandlowerPCRduplicatesLessadditionalnext-generationorSangersequencingneededtocompletegenomesImprovecoverageofGC-andAT-richregionsLoweramplificationbiasimprovescoverageuniformityofGC-andAT-richregions,promoters,lowcomplexityandotherchallengingregionsImprovedoverallcoverageandcoverageuniformityobservedonbothIllumina andIonTorrent™ sequencingplatformsExerciseprecisecontroloverlibraryamplificationKAPAReal-TimeAmplificationKitsallowforlibraryamplificationtobeobservedinrealtimeTerminateamplificationattheoptimalpointforindividualsamplesOptimizelibraryamplificationparametersforhigherthroughputworkflowsAmplifyNGSlibrarieswithindustry-leADIngfidelityKAPAHiFiwasengineeredusingdirectedevolutiontohaveenhancedproofreading(3′-5′exonuclease)activityIndustry-leadingfidelityconfirmedby454sequencingApplications:ApplicationsAmplificationofIllumina libraries(DNAandRNAsequencing)Real-timeamplificationoflibrariespreparedfromlowinput,ChIPDNAandotherprecioussamplesPre-andpost-captureamplificationintargetedcaptureworkflowsKitSpecificationsandContents/Storage:KitSpecificationsandContents/StorageKitscanbestoredforupto12months at-20˚C.KitsincludeKAPAHiFiHotStartReadyMix(2X),aconvenientPCRmastermixcontainingKAPAHiFiHotStartDNAPolymerase,KAPAdNTPs,reactionbuffer,andMgCl2atafinalconcentrationof2.5mM.Kitswithprimersarealsoavailable.Kitsforreal-timelibraryamplificationincludeareal-timeversionoftheKAPAHiFiHotStartReadyMix,aswellasfluorescentstandardstomonitorlibraryamplification.ComponentsSpecificationsSpecDescriptionCompatIBLePlatformIlluminaHiSeq,MiSeq,NextSeq,andGAIIxLibraryTypeDNA,RNA,andbisulfite-treatedDNAStartingMaterialAnyNGSlibrarythatrequiresamplificationSequencingApplicationsWholeGenomeSequencingWholeExomeSequencingTargetedSequencing(custompanels)RNA-SeqChIP-SeqAmpliconSequencing

Kapa biosystems成立于2006年，其生产基地位于南非开普敦。致力于研发、生产下一代PCR（Next-Generation PCR）所需的生物酶。分子进化技术（酶工程）打破了现有的研究局限，为生物研究开创了一片新天地。公司技术团队在蛋白质工程、基因组学、蛋白质组学、合成生物学方面获得非常突出的成绩。Kapa biosystems公司追求科学研究与市场化运作的完美结合，执着于“科技创造完美产品，助力生命科学研究”这一最高宗旨。

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