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Kapa biosystems/Library Preparation for Illumina/KK8234/96 reactions

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KK8234

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Description:Highthroughput,"withbead"LibraryPreparationforIlluminaIt’scomplex,butwehaveitcovered.KAPADNALibraryPreparationKitsforIllumina®sequencingplatformscontainevolvedandoptimallyformulatedenzymesthatenablethehighestoverallcoveragefromtheleastamountoftotalsequencing.KitsareoptimizedtoachievesignificantlyhigherlibraryyieldsandcontainKAPAHiFiforhigh-fidelity,high-efficiencyandlow-biaslibraryamplification.Thisensuresthehighestlibraryandsequencedataquality,particularlyfromlow-inputanddifficultsamplessuchasFFPEandChIPDNA.NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.DownloadourKAPA AdapterandBeadCalculator ForResearchUseOnly.Notforuseindiagnosticprocedures.ProductHighlightsPrevNextAchievegreatermolecularcomplexityHigheryieldsofadapter-ligatedlibrarytranslatestohighermolecularcomplexityFewercyclesofamplificationareneeded,whichresultsinlowerPCRduplicationratesPCR-freeworkflowspossIBLefrominputs≥100ng*PrevNextReduceamplificationbiasandimprovecoverageKAPAHiFireducesPCRbias*ImprovescoverageuniformityofGC-andAT-richregions,promoters,lowcomplexityandotherchallengingregions*PrevNextCreatehigh-qualitylibrariesfromchallengingsamplesHigh-qualitywholeexomesequencingfromlibrariespreparedusingaslittleas10nghumangenomicDNA*Highsuccessrateswith250ngFFPEorless*Routinelibraryconstructionfrom≥100pgChIPDNA*Automatewithpre-validatedscriptsPerkinelmerSciclone®NGSandNGSxBeckmanCoulter®Biomek™FXpandFXdual-podAgilent®TechnologiesNGSWorkstationOptionBEppendorf®epMotion™ 5075TMX *Dataonfile.Applications:ApplicationsWholeGenomeSequencingChIPSequencingWholeExomeSequencingAmpliconSequencingTargetedSequencing(Capture)MethylSequencingKitSpecificationsandContents/Storage:KitSpecificationsandContents/StorageKitscanbestoredforupto18months at-20˚C.Kitsincludereagentsforendrepair,A-tailing,ligationandlibraryamplification.PCR-freeversions(withoutlibraryamplificationreagent)arealsoavailable.“With-bead”kitsincludePEG/NaClsolutionforSPRIbeadcleanups.All96-librarykitsareautomationfriendly.ComponentsSpecificationsSpecDescriptionCompatiblePlatformIlluminaHiSeq,MiSeq,NextSeqandGAIIxLibraryTypeDNAStartingMaterialFragmentedDNAorPCRampliconsInputAmount100pg–5µgSequencingApplicationsWholeGenomeSequencingWholeExomeSequencingTargetedSequencing(custompanels)ChIP-SeqAmpliconSequencingMethyl-Seq

Kapa biosystems成立于2006年，其生产基地位于南非开普敦。致力于研发、生产下一代PCR（Next-Generation PCR）所需的生物酶。分子进化技术（酶工程）打破了现有的研究局限，为生物研究开创了一片新天地。公司技术团队在蛋白质工程、基因组学、蛋白质组学、合成生物学方面获得非常突出的成绩。Kapa biosystems公司追求科学研究与市场化运作的完美结合，执着于“科技创造完美产品，助力生命科学研究”这一最高宗旨。

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