首页 > Norgen Biotek > Norgen Biotek /Total RNA Purification Maxi Kit (Cat. 26800)/8preps/26800

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Norgen Biotek /Total RNA Purification Maxi Kit (Cat. 26800)/8preps/26800

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Extract high quality & purity total RNA including miRNA No phenol step required - isolate all RNA in one fractionRapid processing in under 40 minutes Isolate total RNA from a wide variety of specimensBuffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note Figure 1. Isolate a Diversity of RNA Species. Total RNA was isolated from 10 mL of an overnight E. coli culture using Norgen"s Total RNA Purification Maxi Kit. One microliter of the 2 mL elution was analyzed on an Agilent® 2100 BioAnalyzer RNA Nano 6000 chip. As it can be seen, high quality total RNA was isolated with minimal degradation. As well, a large amount of small RNA (< 200 nt) was recovered, indicating that the kit is able to isolate a diversity of RNA species.]">Total RNA Purification Maxi Kit Figure 1Figure 2. Good Amplification and Consistency of Isolation. Norgen"s Total RNA Purification Maxi Kit was used to isolate total RNA from six different overnight samples of E. coli (10 mL samples). In brief, one microgram of the isolated RNA was subjected to a 20 µL reverse transcription using Invitrogen Superscript III. Three microliters of the reverse transcription was used as the template in a 20 µL qPCR reaction using16S rRNA E. coli specific primers and Bio-Rad iQ mix. As it can be seen, the RNA was detected and successfully amplified in all cases. Furthermore, all samples showed a similar Ct value, indicating consistent isolation.]">Total RNA Purification Maxi Kit Figure 2Total RNA Purification Maxi KitNorgen’s Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.Norgen’s Purification Technology Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Buffer RL (please see the flow chart on page 4). Ethanol is then added to the lysate, and the solution is loaded onto a maxi spin-column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Solution A. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.

Norgen Achieves Propel Certification

Today on November 20th, 2018, we are proud to announced that we have become Propel-Certified through Illumina as a Next Generation Sequencing (NGS) service provider. Norgen's Propel Certification covers both the NextSeq 500 System and the NextSeq 550 System.

PRNewswire

Norgen Biotek Corp. Named Niagara's Best Business

In October 2012 Norgen Biotek Corporation was named Niagara's company of the year at the 2012 Niagara Business Achievement Awards.

St. Catharines Standard Brock University

Norgen Biotek Corp. Achieves Accreditation to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials

In October 2011 Norgen Biotek Corporation achieved successful certification to ISO 15189:2007 for Molecular Diagnostic Testing and Clinical Trials.

Press release

Gary Goodyear, Minister of State for Science and Technology Visits Norgen Biotek

In February 2011 Gary Goodyear, Minister of State for Science and Technology visited Norgen Biotek Corporation announcing funding to develop two projects to create new diagnostic test kits.

Norgen Biotek Corp. Achieves ISO 13485:2003 and ISO 9001:2008 Certification

In November 2009 Norgen Biotek Corporation achieved successful certification to ISO 13485:2003 and ISO 9001:2008.

Press Release

NRC-CNRC – Canada’s Innovative Leaders Award

In October 2003, Norgen Biotek was recognized by the National Research Council (NRC) as one of Canada’s Innovative Leaders for the development of their nucleic acid and protein purification technology. NRC recognized the power of Norgen’s technology, and it’s ability to benefit both the genomics and proteomics industries. In December 2007, Norgen Biotek was recognized as one of Canada’s Top 10 Life Science Companies. Canada's Top 10 Life Science Companies are evaluated by an independent international panel of life science venture capitalists. The panel selects companies based on their attractiveness as an exceptional investment in Canada, and Norgen Biotek was chosen as one of these companies. The prestigious Canada’s Top 10 competition was invented in 1999 to promote the ten most promising life science companies chosen from a pool of entrants by an independent international jury of life science venture capitalists. Based on their expert knowledge of investment in the life science sector, the jury selects companies that offer the best investment and partnership prospects.

National Research Council Canada

2002 Niagara Entrepreneur of the Year Awards Bell Canada Emerging Entrepreneur Award

In 2002, Dr. Haj-Ahmad (President and CEO) was nominated for and won the Bell Canada Emerging Entrepreuner Award for his work at Norgen Biotek. The awards are given to men and women who exemplify the true spirit of entrepreneurship, who through hard work, determination, creativity and resourcefulness have established new ventures or lead existing ones into renewed growth.

Niagara Entrepreneur of the Year Awards