Extract total RNA (including microRNA) from FFPE samplesNo phenol extraction stepIncludes DNase for optional on-column DNA removalIsolated RNA is of the highest quality and integrityIsolate a diversity of RNA speciesPurified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen"s NGS servicesBuffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application NoteRelated ServicesNGS RNA Isolation Figure 1. High Quality and Yield of Total RNA. Norgen"s FFPE RNA Purification Kit isolates FFPE RNA that exceeds both yield and quality of competitors. Total RNA was isolated from one slice of hamster FFPE kidney section (20 micron thickness) using Norgen"s FFPE RNA Purification Kit and a leading competitors kit. One microliter of the 50 µL purified RNA was then resolved on an Agilent 2100 BioAnalzyer using an RNA Nano 6000 chip. As it can be seen, Norgen not only isolated higher yields of total RNA, but the RNA was also of a higher quality as evidenced by the higher RIN values obtained with Norgen"s RNA.]">FFPE RNA Purification Kit Figure 1Figure 2. Higher Yield of FFPE RNA Isolated by Norgen"s FFPE RNA Purification Kit. Norgen"s FFPE RNA Purification Kit isolates FFPE RNA that exceeds the yield of competitors. Total RNA was isolated from one slice of hamster FFPE kidney section (20 micron thickness) using Norgen"s FFPE RNA Purification Kit and two leading competitor"s kits. Eighteen isolations were performed for each product. The graph demonstrates the mean yield of RNA according to spectrophotometry for 18 sample replicates.Vertical bars represent the standard deviation. Norgen"s kit consistently purified total RNA with a higher yield than for those obtained using the market competitor"s kits.]">FFPE RNA Purification Kit Figure 2Figure 3. High Yield of a Diversity of RNA Species. Norgen"s FFPE RNA Purification Kit effectively recovers all sizes of RNA from large mRNA to small RNA including microRNAs. Total RNA was isolated from one slice of hamster FFPE kidney section (20 micron thickness) using Norgens FFPE RNA Purification Kit and a leading competitors kit. Five microliters of the 50 µL purified RNA was then used as the template in a 20 µL reverse transcription reaction with oligo dT primer or miR-21 stem-loop reverse primer. Two microliters of the RT reaction was then used in a 15 µL qPCR reaction for detecting the beta-actin gene (Panel A) and for detecting miR-21(Panel B), respectively. In both graphs the blue lines correspond to Norgen isolated-RNA and the green lines correspond to competitor-isolated RNA. As it can be seen, Norgen"s kit isolated higher yields of RNA in both cases, as indicated by the lower Ct values of the blue lines. Also, Norgen"s kit successfully isolated not only large RNA (Panel A) but also microRNA (Panel B), indicating the diversity of RNA species isolated.]">FFPE RNA Purification Kit Figure 3Figure 4. Excellent Performance in Downstream Application such as RT-qPCR. Norgen"s FFPE RNA Purification Kit effectively recovers all sizes of RNA, from large mRNA to small RNA including microRNA, that perform effectively in sensitive applications such as RT-qPCR. Total RNA was isolated from equal amounts of an FFPE tissue sample using Norgen"s FFPE RNA Purification Kit and two market competitors. The graph demonstrates the mean delta Ct value for 18 sample replicates. Vertical bars represent the standard deviation. Norgen"s kit consistently isolates RNA of a higher yield and quality than that obtained by the two leading market competitors as indicated by the larger delta Ct values. Not only were the large RNA species detected including rRNA and mRNA, but small RNA species (microRNA) were also detected from the sample, indicating the diversity of RNA species isolated.]">FFPE RNA Purification Kit Figure 4FFPE RNA Purification KitNorgen’s FFPE RNA Purification Kit provides a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time.