首页 > Norgen Biotek > Norgen Biotek /Plant microRNA Purification Kit (Cat. 54700)/25preps/54700

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Norgen Biotek /Plant microRNA Purification Kit (Cat. 54700)/25preps/54700

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Fast and easy processing using a rapid spin-column formatNo phenol or chloroform extractionsIsolate all small RNA molecules (<200 nt)Minimal contamination from large RNA molecules and genomic DNAHigh quality small RNA can be used in various downstream applications Figure 1. Fractionation of Large and Small RNA. Large RNA and small RNA were sequentially purified using Norgen"s Plant miRNA Purification Kit from 50 mg of raspberry leaf tissue and the RNA profile was compared with the RNA isolated using Norgen"s Plant/Fungi Total RNA Purification Kit (Cat. #25800). For visualization, 10 µL of RNA from 50 µL of RNA elution was loaded on 2% 1x MOPS agarose gel. Norgen"s kit was able to isolate both the large and small RNA fractions, and the small RNA fraction does not contain any of the large RNA species.]">Plant microRNA Purification Kit Figure 1Figure 2. Efficiency of the concentration of miRNA using Norgen"s Plant miRNA Purification Kit to detect three plant miRNAs. Small RNA was concentrated from 50 mg raspberry using Norgen"s Plant miRNA Purification Kit. Three µL of the elution was directly used for cDNA generation and 3 µL of cDNA was used for the CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad). Blue: concentrated small RNA from Norgen"s Plant miRNA Purification Kit, Red: Total RNA from Plant Total RNA Purification Kit (Cat. 25800), Circle: +RT, Cross: - RT.]">Plant microRNA Purification Kit Figure 2Figure 3. Isolation of Concentrated RNA that can be used in Sensitive Downstream Applications. Three different miRNA purification methods were compared for their ability to isolate and detect plant miRNA. miRNA was purified from grape using three miRNA purification kits according to the manufactures manual. Next, 3 µL of the elution was directly used for cDNA generation and 3 µL of cDNA was used for the CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad) to detect the miRNA 159a. As it can be seen, only the miRNA purified using Norgen"s kit (blue lines) was amplified, while the miRNA isolated using the other 2 kits failed to amplify (red and green lines). Both of the competitors kits rely on phenol to isolate the miRNA, and therefore residual phenol is likely interfering with the amplification. Norgen"s kit isolates high yields of good quality miRNA without the use of phenol. Blue: Norgen Plant miRNA, Red: Competitor Q, Trizol: Green.]">Plant microRNA Purification Kit Figure 3Plant microRNA Purification KitNorgen’s Plant microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured plant cells or plant tissues. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. The small RNA molecules isolated using Norgen’s Plant microRNA Purification Kit can be used in various downstream applications relating to gene regulation and functional analysis, including RT-PCR, miRNA sequencing, northern blotting and microarray analysis.

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Today on November 20th, 2018, we are proud to announced that we have become Propel-Certified through Illumina as a Next Generation Sequencing (NGS) service provider. Norgen's Propel Certification covers both the NextSeq 500 System and the NextSeq 550 System.

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In October 2012 Norgen Biotek Corporation was named Niagara's company of the year at the 2012 Niagara Business Achievement Awards.

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In February 2011 Gary Goodyear, Minister of State for Science and Technology visited Norgen Biotek Corporation announcing funding to develop two projects to create new diagnostic test kits.

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NRC-CNRC – Canada’s Innovative Leaders Award

In October 2003, Norgen Biotek was recognized by the National Research Council (NRC) as one of Canada’s Innovative Leaders for the development of their nucleic acid and protein purification technology. NRC recognized the power of Norgen’s technology, and it’s ability to benefit both the genomics and proteomics industries. In December 2007, Norgen Biotek was recognized as one of Canada’s Top 10 Life Science Companies. Canada's Top 10 Life Science Companies are evaluated by an independent international panel of life science venture capitalists. The panel selects companies based on their attractiveness as an exceptional investment in Canada, and Norgen Biotek was chosen as one of these companies. The prestigious Canada’s Top 10 competition was invented in 1999 to promote the ten most promising life science companies chosen from a pool of entrants by an independent international jury of life science venture capitalists. Based on their expert knowledge of investment in the life science sector, the jury selects companies that offer the best investment and partnership prospects.

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2002 Niagara Entrepreneur of the Year Awards Bell Canada Emerging Entrepreneur Award

In 2002, Dr. Haj-Ahmad (President and CEO) was nominated for and won the Bell Canada Emerging Entrepreuner Award for his work at Norgen Biotek. The awards are given to men and women who exemplify the true spirit of entrepreneurship, who through hard work, determination, creativity and resourcefulness have established new ventures or lead existing ones into renewed growth.

Niagara Entrepreneur of the Year Awards