Extract high quality & purity total RNA including miRNA Isolate from a wide variety of animal tissues No phenol step required - isolate all RNA in one fractionCompatible with tissues stored in RNAlater® or Trizol®  Convenient & fast spin column formatBuffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note Figure 1. High Quality of Isolated RNA with Complete Size Range. Unlike most competitors kits, Norgen"s Animal Tissue RNA Purification Kit allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA without the use of phenol. Total RNA was isolated from 25 mg samples of rat muscle using Norgens Animal Tissue RNA Purification Kit and a leading competitor"s kit. Five microliters of the 50 µL RNA eluted from each kit was then resolved on a 1.2% formaldehyde-agarose gel. As it can be seen, only Norgens kit was able to isolate the full diversity of RNA species, including all the small RNA species]">Animal Tissue RNA Purification Kit Figure 1Figure 2. High Quality RNA Purification from a Diversity of Tissues.Norgens Animal Tissue RNA Purification Kit isolates high quality RNA from a diversity of tissues including both tissues with high RNA content and fibrous tissues. Total RNA was successfully isolated from various tissue samples including 10 mg of spleen (Panel C), 10 mg of kidney (Panel D) and 25 mg of fiber-rich muscle (Panel B) using Norgens Animal Tissue RNA Purification Kit and a leading competitor"s kit (Panel A). All RNA samples were eluted in a 50 µL volume. One microliter of each of the purified RNA samples was then analyzed using an Agilent BioAnalyzeron an RNA Nano 6000 Chip. All RNA samples resolved well in the Bioanalyzer suggesting high RNA quality. Moreover, small RNA could be observed in all the RNA samples isolated using Norgens kit (Panels B-D, circled), whereas no small RNA was present in the samples isolated using a competitors kit (Panel A).]">Animal Tissue RNA Purification Kit Figure 2Figure 3. Total RNA with High Yield of microRNA Species. Unlike most competitor kits that exclude RNA species of size < 200 nt, Norgens Animal Tissue RNA Purification Kit isolates the complete size range of total RNA including small RNAs. Total RNA was isolated from 25 mg of a rat muscle sample using Norgens Animal Tissue RNA Purification Kit and a leading competitors kit as shown in Figure 1. Two microliters of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT. Two microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the rat miR-21 (Panel A) and the beta-actin transcripts (Panel B), respectively. In both graphs the blue lines correspond to Norgen isolated-RNA and the red lines correspond to competitor-isolated RNA. As it can be seen, Norgens kit isolated much higher yields of microRNA, as indicated by the lower Ctvalue of the blue line in Panel A. The kits isolated similar yields of mRNA as seen by the similar Ctvalues in Panel B. NTC corresponds to no-template control.]">Animal Tissue RNA Purification Kit Figure 3Figure 4. Compatibility with Tissues Stored in RNAlater®. Norgens Animal Tissue RNA Purification Kit can isolate total RNA from both flash frozen tissues and tissues stored in RNA preservation media such as RNAlater®. Total RNA wasisolated from 10 mg of a hamster kidney sample, either flash frozen or stored in RNAlater®, using Norgens Animal Tissue RNA Purification Kit and a leading competitors kit. Three microliters of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT. One microliter of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the hamster miR-21 (Panel A) and the beta-actin transcripts (Panel B), respectively. In both graphs the blue lines correspond to Norgen isolated-RNA and the red lines correspond to competitor-isolated RNA, both from RNAlater®-preserved samples. The green lines correspond to Norgen isolated-RNA from flash frozen samples. As it can be seen, Norgens kit isolated similar amounts of both mRNA and microRNA from either frozen or RNAlater®-preserved samples, as shown by the similar Ct values of the blue and green lines. In addition, Norgens kit isolated much higher yields of microRNA than the competitors kit, as indicated by the lower Ctvalue of the blue and green lines in Panel A. The kits isolated similar yields of mRNA as seen by the similar Ctvalues in Panel B. NTC corresponds to no-template control.]">Animal Tissue RNA Purification Kit Figure 4Animal Tissue RNA Purification KitThis kit is suitable for the isolation of total RNA from a range of animal tissues such as liver and spleen, as well as difficult fibrous tissues such as heart, muscle, intestine, etc.  Briefly, the tissue of interest is first lysed using Buffer RL, followed by treatment with the provided Proteinase K which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins and connective tissues. The purified RNA is of the highest quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.