首页 > Trevigen > Trevigen/CardioTACS™ In Situ Apoptosis Detection Kit/4827-30-K/30 Samples

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Trevigen/CardioTACS™ In Situ Apoptosis Detection Kit/4827-30-K/30 Samples

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4827-30-K

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DescriptionThehighcellularityofcardiactissuepresentsproblemsinpermeABIlization,sotheCardioTACS™Kitcomeswithtwopermeabilizationreagentstoprovideoptions.ThekitisbasedonDNAend-labelingusingterminaldeoxynucleotidyltransferase(TdT)andamodifiednucleotidethatissubsequentlydetectedusingourTACSBlueLabel™detectionsystem.TrevigenhasdevelopedanexclusiveapoptosisgradeTdTenzymethatwhencoupledwiththeTACSBlueLabel™solution,provideslabeling20to50timesmoresensitivethanstandarddiaminobenzidine(DAB)labelingmethods.Toensureeaseofdatainterpretationwhenthenumbersofapoptoticcellsarelow,CardioTACS™includesNuclearFastRedCounterstainwhichprovidessuperbcontrasttotheTACSBlueLabel™inapoptoticcells.Inaddition,thekitincludesTACS-Nuclease™togenerateapositivecontrolinyourownsample.Thepositivecontrolprovidesaninternalcontrolforpermeabilizationandlabelingsoadditionaloptimizationisnominalandthedatacanbeinterpretedwithconfidence.Thiscompletekitprovidesallthereagentsrequiredforlabelingincludingtwopermeabilizationreagents,labelinganddetectionreagents,stopbuffer,counterstain,TACS-Nuclease™reagent,andaprotocoldetailingincubationtimesandreagentconcentrations. FEATURES:Performancetestedonheart-derivedsamples.IncludesbothProteinaseKandexclusiveCytonin™non-enzymaticpermeabilizationreagents.IncludesTACS-Nuclease™solutionforpreparingsample-dependentpositivecontrols.Helpsresolveuniqueproblemsencounteredwhendetectingapoptoticcardiaccells.APPLICATIONS:Insitudetectionofapoptosisinfixedfrozen,paraffinembedded,orplasticembeddedcardiaccellsandtissues.Assistsintheidentificationofapoptoticmorphologies.Catalog#4827-30-Kincludes:CatalogNumberDescriptionQty4800-30-01ProteinaseK14800-30-06Streptavidin-HRP14800-30-11TACS-BlueLabel™14800-30-12BlueStrep-HRPDiluent14800-30-15TACS-Nuclease™14800-30-16TACS-Nuclease™Buffer14800-30-17NuclearFastRed14810-30-02TACS®2TdTLabelingBuffer14810-30-03TACS®2TdTStopBuffer14810-30-04TACS®2TdTdNTP14810-30-05TdTEnzyme14810-30-1450xManganeseCation14876-05-01Cytonin™1 TheInfluenceofAgeonSerumConcentrationsofCardiacTroponinI:ResultsinRats,Monkeys,andCommercialSeraEugeneH.Herman,AlanKnapton,YongminLiu,StevenE.Lipshultz,JoelEstis,JohnTodd,RuthA.Woodward,ThomasCochran,JunZhang,andMiriamC.PoirierToxicolPathol,Jul2014;42:888–896.http://tpx.sagepub.com/cgi/content/abstract/42/5/888“GSK-3βheterozygousknockoutiscardioprotectiveinaknockinmousemodeloffamilialdilatedcardiomyopathy”“RashaM.S.M.Mohamed,SachioMorimoto,IslamA.A.E.-H.Ibrahim,Dong-YunZhan,Cheng-KunDu,MasakiArioka,TatsuyaYoshihara,FumiTakahashi-Yanaga,andToshiyukiSasaguri”AmJPhysiolHeartCircPhysiol,Jun2016;310:H1808–H1815http://ajpheart.physiology.org/cgi/content/abstract/310/11/H1808“Phosphodiesterase5InhibitionLimitsDoxorubicin-inducedHeartFailurebyAttenuatingProteinKinaseGIαOxidation”“OleksandraPrysyazhna,JosephRobertBurgoyne,JennaScotcher,StevenGrover,DavidKass,andPhilipEaton”J.Biol.Chem.,Aug2016;291:17427–17436.http://www.jbc.org/cgi/content/abstract/291/33/17427

Trevigen公司的3D和2D细胞培养产品的设计，开发，生产，合格，由我们Cultrex支持®实验室工作人员。我们的产品包括替代基质胶®以及合格的三维培养几个独特类型的基底膜提取物（BME）矩阵，干细胞化培养和球体。提供的其他基质包括层粘连蛋白I，胶原蛋白I，胶原蛋白IV，玻连蛋白，纤连蛋白，聚-D-赖氨酸和聚-L-赖氨酸。