首页 > Trevigen > Trevigen/Superoxide Dismutase Assay Kit (Cuvette)/7500-100-K/100 reactions

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Trevigen/Superoxide Dismutase Assay Kit (Cuvette)/7500-100-K/100 reactions

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7500-100-K

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DescriptionIntheSuperoxideDismutaseAssay,ionsgeneratedfromtheconversionofxanthinetouricacid,andhydrogenperoxidebyxanthineoxidase(XOD),convertNBTtoNBT-diformazan.NBT-diformazanabsorbslightat550nm.SODsreducesuperoxideionconcentrationsandtherebylowertherateofNBT-diformazanformation.TheextentofreductionintheappearanceofNBT-diformazanisameasureofSODactivitypresentinexperimentalsamples.Theassayisfreeofinterferencebyothercatalyticactivities,andisidealforassayingSODinmammaliancelllysates.Thekitcontainstheproperlysisbufferandthereagentsneededfor100experimentaltests,50positivecontrols,and50negativecontrols.UnlikesomeotherassaykitsforSOD,thissystemisnotgreatlydisturbedbytracemetals.Eachassayrequiresonlyaboutfiveminutes,andafterasimplecalculation,thepercentinhibitionoftheformationofNBT-diformazanbySODisconvertedtotherelativeactivityofthesample.FEATURES:Suitableformammaliancells.Eachsampletakesonly5minutes.ContainsSODfor50positivecontrols.Suitablefortheassayof(Mn2+)-SOD,(Fe2+)-SOD,and(Cu/Zn)–SOD.APPLICATIONS:CalculatingSODactivitybymeasuringthereductionofNBT-diformazanincellextracts. Catalog#7500-100-Kincludes:CatalogNumberDescriptionQty7500-100-01SuperOxideDismutase17500-100-05XODSolution17500-100-0225XSODReactionBuffer17500-100-03XanthineSolution17500-100-04NBTSolution17500-100-0620XCellLysisSolution1RosiglitazoneCausesCardiotoxicityviaPeroxisomeProliferator-ActivatedReceptor{gamma}-IndependentMitochondrialOxidativeStressinMouseHeartsHuameiHe,HaiTao,HuiXiong,ShengZhongDuan,FrancisX.McGowan,Jr.,RichardM.Mortensen,andJamesA.BalschiToxicol.Sci.,Apr2014;138:468–481.http://toxsci.oxfordjournals.org/cgi/content/abstract/138/2/468Protectiveactivityofanovelresveratrolanalogue,HS-1793,againstDNAdamagein137Cs-irrADIatedCHO-K1cellsJeong,M.H.,Yang,K.M.,Jeong,D.H.,Lee,C.G.,Oh,S.J.,Jeong,S.K.,Lee,K.W.,Jo,Y.R.andJo,W.S.J.RadiatRes,May2014;55:464–47http://jrr.oxfordjournals.org/cgi/content/abstract/55/3/464

Trevigen公司的3D和2D细胞培养产品的设计，开发，生产，合格，由我们Cultrex支持®实验室工作人员。我们的产品包括替代基质胶®以及合格的三维培养几个独特类型的基底膜提取物（BME）矩阵，干细胞化培养和球体。提供的其他基质包括层粘连蛋白I，胶原蛋白I，胶原蛋白IV，玻连蛋白，纤连蛋白，聚-D-赖氨酸和聚-L-赖氨酸。