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Trevigen/PARP/Apoptosis Colorimetric Assay Kit/4684-096-K/96 tests
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DescriptionThisELISAbasedassaydetectspoly(ADP-ribose)depositedbyPARP-1ontoimmobilizedhistonesina96-wellformat.Ananti-PARmonoclonaldetectingantibodyfollowedbyadditionofagoatanti-rabbitIgG-HRPsecondaryandacolorimetricHRPsubstrateyieldsrelativeabsorbancethatcorrelateswithPARP-1activity. DuringapoptosisPARP-1iscleavedwitharesultingdropinactivity.Etoposideisincludedasacontrolapoptosisinducer.FEATURES:Colorimetricreadout96wellformatHighlysensitive–detects0.1mUPARP~500cellsDynamicrangebetween0.1to10mUPARPRequires10-100ngextractfordetectionAssayTime~3hrsAPPLICATIONS:MeasurePARP-1activityincellextractsbeforeandafterapoptosis.ForthescreeningofcandidatePARP-1inhibitorsanddeterminationofIC50values.Catalog#4684-096-Kincludes:CatalogNumberDescriptionQty4671-096-0610XActivatedDNA14684-096-01PARP-HSA,10mUnits/µl14684-096-0220mMNAD14684-096-04Anti-PARmonoclonalAntibody14684-096-05Goatanti-mouseIgG-HRP14684-096-0610mMEtoposide14684-096-0720XI-PARAssayBuffer14684-096-035XAntibodyDiluent14684-096-PHistone-CoatedNaturalStripWellPlate,I-PAR14822-96-08TACS-Sapphire1“UltravioletRADIationSignalingthroughTLR4/MyD88ConstrainsDNARepairandPlaysaRoleinCutaneousImmunosuppression”ErinHarberts,HuaZhou,RitaFishelevich,JuanLiu,andAnthonyA.GaspariJ.Immunol.,Apr2015;194:3127–3135.http://www.jimmunol.org/cgi/content/abstract/194/7/3127“BokIsNotPro-ApoptoticButSuppressesPolyADP-RibosePolymerase-DependentCellDeathPathwaysandProtectsagainstExcitotoxicandSeizure-InducedNeuronalInjury”“BeatriceD’Orsi,TobiasEngel,ShonaPfeiffer,SaheliNandi,ThomasKaufmann,DavidC.Henshall,andJochenH.M.Prehn”J.Neurosci.,Apr2016;36:4564–4578http://www.jneurosci.org/cgi/content/abstract/36/16/4564NAD+repletionimprovesmusclefunctioninmusculardystrophyandcountersglobalPARylation“DongryeolRyu,HongboZhang,EduardoR.Ropelle,VincenzoSorrentino,DaviA.G.Mázala,LaurentMouchiroud,PhilipL.Marshall,MatthewD.Campbell,AmirSafiAli,GaryM.Knowels,StéphanieBellemin,ShamaR.Iyer,XuWang,KarimGariani,AnthonyA.Sauve,CarlesCantó,KevinE.Conley,LudivineWalter,RichardM.Lovering,EvaR.Chin,BernardJ.Jasmin,DavidJ.Marcinek,KeirJ.Menzies,andJohanAuwerx”ScienceTranslationalMedicine,Oct2016;8:361ra139http://stm.sciencemag.org/cgi/content/abstract/8/361/361ra139
Trevigen公司的3D和2D细胞培养产品的设计,开发,生产,合格,由我们Cultrex支持®实验室工作人员。我们的产品包括替代基质胶®以及合格的三维培养几个独特类型的基底膜提取物(BME)矩阵,干细胞化培养和球体。提供的其他基质包括层粘连蛋白I,胶原蛋白I,胶原蛋白IV,玻连蛋白,纤连蛋白,聚-D-赖氨酸和聚-L-赖氨酸。
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