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NeoBiotechnologies/Bcl-2 (Apoptosis & Follicular Lymphoma Marker)/596-MSM11-P0
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Product DetailsSynonymsApoptosis regulator Bcl-2, B-cell CLL/lymphoma-2Positive ControlJurkat, K562, HL-60 or HeLa cells. Tonsil or follicular lymphomas.Known Applications & Suggested DilutionsFlow Cyt, IF, Western Blot, IHC-P More Details Flow Cytometry (1-2ug/million cells)Immunofluorescence (1-2ug/ml)Western Blot (1-2ug/ml)Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA Buffer, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.ImmunogenRecombinant full-length human BCL2 proteinCellular LocalizationOuter mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. Species ReactivityHuman.Host / Ig IsotypeMouse / IgG1, kappaMol. Weight of Antigen25-26kDa

NeoBio的SFN(stratifin)是一种p53诱导的肿瘤抑制基因,其响应DNA损伤而被激活,通过阻止cdc2-cyclin B1复合物进入细胞核而导致细胞周期停滞在G2期。在乳腺癌,肺癌,前列腺癌,肝癌和胃癌中被灭活。当在上皮性卵巢癌中观察到其下调时,其与不良预后相关。SFN表达可能有助于癌细胞增殖以及人类胃肠道癌的发展和/或进展。

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