.img-style{heigth:450px;width:450px;}Overview:Abetterwaytoisolateexosomes“WethereforepursuedtheExoQuick®methodforfurtherstudy,asthesesamplesrequiredmuchlesssampleinput,akeybenefitwhenworkingwithclinicalsamplesandmousemodels1.” Needexosomes?SBI’sExoQuick,originalformulation,enableshigh-throughput,quantitativeisolationofexosomesfromlowvolumes(aslittleas100µl)ofserum,plasma,orascitesfluid.CompatIBLewithawidevarietyofdownstreamapplications,ExoQuickisaneffectiveandprovenalternativetoultracentrifugation1-3.ExoQuick’sfast,ultracentrifugation-freemethod:SavestimeandlaborIseasilyscalableConservesprecioussampleDelivershighyieldsoffunctional,highqualityexosomesCanbeusedtoisolateexosomesforawiderangeofdownstreamapplications,includingBioMarkerstudiesExosomalmiRNAprofilingExosomalproteomicsExosomallipidomics/metabolomicsFunctionalstudies,suchasincell-to-cellsignalingBasicBIOLOGy,suchasroleintumOrigenesisExoQuickisaproprietarypolymerthatgentlyprecipitatesexosomes.First,pre-clearyoursamplesofcellsandcellulardebris,andthensimplyaddtheappropriateamountofExoQuicktoyourclearedbiofluid,refrigerate,andcentrifuge(seetheproductmanualforprotocoldetails).Yourexosomeswillbeinthepellet,readyforresUSPensioninanappropriatesolution.BiofluidSamplevolumeExoQuick-TCVolumeTissueculturemedia,urine,cerebrospinalfluid(CSF),etc.5mL1mLInelectronmicroscopystudies,exosomesisolatedwithExoQuickappearsimilartoexosomesisolatedusingultracentrifugation1-2,andtheseexosomesarealsoactiveinnumerousfunctionalassays1-3.ExosomesisolatedwithExoQuickcanbeusedforalltypesofproteinprofilingandproteincharacterizationstudies,suchasmassspectrometry,Westernblotting,ELISA,andmore.HigherproteinyieldsareachievedbyExoQuickpurificationthanbychromatography,DynaBeads,orultracentrifugation.ExosomesisolatedwithExoQuickalsoprovideexcellentsamplesforstudyingexosome-associatednucleicacidssuchasmicroRNAs,siRNAs,andevenmRNA.QuantitativeanalyticaltechniquessuchasqPCR,microarraystudies,andnext-generationsequencingareallcompatiblewithnucleicacidsisolatedfromExoQuick-purifiedexosomes.Backedbyagrowingnumberofpublications,ExoQuickisoftenthebestoptionforresearchersworkingwithlowsamplevolumes,suchasclinicalresearchsamplesorsmallanimalmodels.ExoQuickexosomeisolationmethodsarepatentedtechnologies4.ChoosetherightExoQuickforyourbiofluid:Catalog#ProductBiofluidEXOQ5A-1,EXOQ20A-1TheOriginalExoQuickForserum,plasma,andascitesfluidEXOTC10A-1,EXOTC50A-1ExoQuick-TC®ForallotherbiofluidsEXOLP5A-1ExoQuick-LPForremovingcontaminatinglipoproteinparticlesfromplasmaorserumbeforeExoQuickprecipitationEXOQ5TM-1ExoQuickPlasmaPrepwithThrombinForde-fibrinatingplasmabeforeexosomeisolationforefficientrecoveryandhighyieldsEXOCG50A-1ExoQuick-CGForpreparingexosomesusedinpre-clinicalinvivoapplicationsEQPL10A-1,EQPL10TCExoQuickPLUSandExoQuick-TCPLUSForsensitiveapplicationssuchasmassspectrometry,exosomelabeling,andinvivo/exvivoexosomedeliveryChoosebetweenExoQuick/ExoQuick-TCandExoQuickPLUS/ExoQuick-TCPLUSbasedonyourapplicationExoQuickExoQuick-TCExoQuickPLUSExoQuick-TCPLUSProteinDetectionWesternblottingforgeneralexosomemarkers(e.g.CD9,CD63,CD81,TSG101,Alix)••••••••••High-sensitivityWesternblotting(e.g.lowabundancebiomarkers)••••••••qPCRAnalysisqPCRofcodingandnon-codingRNAs(e.g.mRNA,miRNA,andlncRNA)••••••••••High-throughputBiomarkerDiscoveryRNA-seqofexosomalRNAs••••••••••Massspectrometryofexosomalproteins••••••••Lipidomics/metabolomicsofexosomalcargo••••••••ExosomeLabeling••••••••Invivo/exvivoExosomeDelivery•••••••••••••Highlyrecommended,•NotrecommendedREFERENCESChughPE,etal.SystemicallyCirculatingViralandTumor-DerivedMicroRNAsinKSHV-AssociatedMalignancies.PLoSPathog.2013.9(7):e1003484.PMCID:PMC3715412.UmezuT,etal.LeukemiacelltoendothelialcellcommunicationviaexosomalmiRNAs.Oncogene.2013May30.32(22):2747-55.PMID:22797057.SohelMM,etal.ExosomalandNon-ExosomalTransportofExtra-CellularmicroRNAsinFollicularFluid:ImplicationsforBovineOocyteDevelopmentalCompetence.PLoSOne.2013Nov4.8(11):e78505.PMCID:PMC3817212.AntesT,etal.MethodsforMicrovesicleIsolationandSelectiveRemoval.PatentNo.:US9,005,888B2.HowItWorks:High-throughput,quantitativeexosomerecoveryExoQuickcanbeusedtopurifyexosomesfromplasma1,serum2,andmalignantascites3.Withasimpleworkflowinvolvingminimalhands-ontimeandlowinputsamplevolumerequirements,ExoQuickisanexcellentoptionforresearcherswhoneedtopurifymultipleexosomesamplesand/orsamplesfromsmallanimalmodelsorclinicalresearchsamples.Toisolateexosomesfromclearedserum,plasma,orascitesfluid,simply:AddanappropriatevolumeofExoQuicktoaslittleas100µlsampleIncubateforatleastonehourat4°CIsolateexosomeswitha30-minutelow-speedspin(1500g).Isolatedexosomescanbefoundinthepelletandresuspendedinanappropriatesolution.Youcanverifythepresenceofexosomeswithanumberofdifferentmethods,includingWesternblottingforgeneralexosomemarkers(CD63,CD9,CD81,andHSP70),NanoSightanalysis,orEM(learnaboutdifferentwaystodetectexosomesandmoreinourExosomeBasicsGuide).TheBottomLineWithExoQuick,youcanobtainhigh-qualityexosomesfrommostbiofluidsusingaprotocolthatcaneasilybeperformedonmultiplesamplesandrequiresverylowvolumesofinputsample.REFERENCESChughPE,etal.SystemicallyCirculatingViralandTumor-DerivedMicroRNAsinKSHV-AssociatedMalignancies.PLoSPathog.2013.9(7):e1003484.PMCID:PMC3715412.EppleLM,etal.MedulloblastomaExosomeProteomicsYieldFunctionalRolesforExtracellularVesicles.PLoSONE.2012.7(7):e42064.PMCID:PMC3407172.Asfeaturedin:ExosomeIsolationforProteomicAnalysesandRNAProfilingDouglasD.Taylor,WolfgangZachariasandCicekGercel-Taylor,Serum/PlasmaProteomics,MethodsinMolecularBiology,2011,Volume728,Part4,235-246.Citations:Nocera,AL,etal.(2017)ExosomesmediateinterepithelialtransferoffunctionalP-glycoproteininchronicrhinosinusitiswithnasalpolyps.Laryngoscope.2017May9;.PMID:28485529Barteneva,NS,etal.(2017)Extracellularvesiclesingastrointestinalcancerinconjunctionwithmicrobiota:OntheborderofKingdoms.Biochim.Biophys.Acta.2017Jun29;.PMID:28669749McCommis,KS,etal.(2017)Targetingthemitochondrialpyruvatecarrierattenuatesfibrosisinamousemodelofnonalcoholicsteatohepatitis.Hepatology.2017May1;65(5):1543-1556.PMID:28027586Oliveira-Rodríguez,M,etal.(2017)Point-of-caredetectionofextracellularvesicles:Sensitivityoptimizationandmultiple-targetdetection.BiosensBioelectron.2017Jan15;87:38-45.PMID:27517736Street,JM,etal.(2017)UrineExosomes:AnEmergingTroveofBiomarkers..AdvClinChem.2017Jan6;78:103-122.PMID:28057185Yap,T,etal.(2017)Oralswirlsamples–arobustsourceofmicroRNAprotectedbyextracellularvesicles..OralDis.2017Apr1;23(3):312-317.PMID:27796067CUI,C,etal.(2017)结直肠癌患者血清外泌体代替肿瘤组织检测K-Ras基因突变的研究.万方数据资源系统.;22(5).Link:万方数据资源系统Lannigan,J&Erdbruegger,U.(2017)Imagingflowcytometryforthecharacterizationofextracellularvesicles..Methods.2017Jan1;112:55-67.PMID:27721015Elshelmani,H&Rani,S.(2017)ExosomalMicroRNADiscoveryinAge-RelatedMacularDegeneration.MethodsMol.Biol..2017Nov9;1509:93-113.PMID:27826921Mullins,RJ,etal.(2017)ExosomalbiomarkersofbraininsulinresistanceassociatedwithregionalatrophyinAlzheimer’sdisease.HumBrainMapp.2017Apr1;38(4):1933-1940.PMID:28105773Qi,H,etal.(2017)Usingendogenousligandsfordirectsuperparamagneticnanoparticlecluster-basedbodyfluidexosomeseparation.RSCAdvances.2016Nov27;7:2926-2933.Link:RSCAdvancesTakeshita,F.(2017)MicroRNAs:EmergingNovelTargetsofCancerTherapies.Book.;.Link:BookCheng,R,etal.(2017)SAT-122-IntegratedanalysisofexosomalmicroRNA,gene,andpathwayregulatorynetworksinfibrosisandhepatocarcinogenesis.JournalofHepatology.;66(1):S638.Link:JournalofHepatologyCho,YE,etal.(2017)Increasedliver-specificproteinsincirculatingextracellularvesiclesaspotentialbiomarkersfordrug-andalcohol-inducedliverinjury.PLoSONE.2017Feb22;12(2):e0172463.PMID:28225807Wu,Q,etal.(2017)Suppressionofendothelialcellmigrationbytumorassociatedmacrophage-derivedexosomesisreversedbyepithelialovariancancerexosomallncRNA.CancerCellInt..2017Jun8;17:62.PMID:28592924Cho,YE,etal.(2017)CirculatingPlasmaandExosomalmicroRNAsasIndicatorsofDrug-InducedOrganInjuryinRodentModels.BiomolTher(Seoul).2017Jul1;25(4):367-373.PMID:28208010Russo,F,etal.(2017)CirculatingNoncodingRNAsasClinicalBiomarkers.Book.;.Link:BookHuang,Z,etal.(2017)AnovelserummicroRNAsignaturetoscreenesophagealsquamouscellcarcinoma.CancerMed.2017Jan1;6(1):109-119.PMID:28035762BMoyron,R,etal.(2017)Differentialproteinexpressioninexosomalsamplestakenfromtraumapatients.ProteomicsClinAppl.2017May25;.PMID:28544811Huang-Doran,I,Zhang,CY&Vidal-Puig,A.(2017)ExtracellularVesicles:NovelMediatorsofCellCommunicationInMetabolicDisease..TrendsEndocrinol.Metab..2017Jan1;28(1):3-18.PMID:27810172

美国SBI代理

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

美国SBI代理

ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1