Monitor and measure the expression of pluripotency markersWith SBI’s line of pGreenFire Pluripotency Response Reporter Vectors, you can monitor and measure the expression of pluripotency markers in live cells in real time. These vectors leverage our reliable lentivector technology and save you time—the pre-built reporters come as ready-to-package lentivector plasmids, with some reporters also available as ready-to-transduce, pre-packaged virus. All of our Response Reporters deliver great performance in both human and mouse cells.The Sox2 SRR2 pGreenFire (EF1α-puro) Response Reporter uses the Sox2-responsive SRR2 enhancer with a mouse CMV promoter to co-express GFP and luciferase. Co-expression is mediated by a T2 element. The minimal CMV promoter alone delivers negligible expression, but when downstream of SSR2 enhancer elements, drives expression of dscGFP and luciferase under conditions where Sox2 is also expressed. The result is the ability to quantitatively measure Sox2 expression by fluorescence and luciferase activity.The vector also includes a constitutively-expressed puromycin marker (EF1α promoter) to simplify cell line construction. Or choose a constitutively expressed neomycin marker (Cat.# SR20071-PA-N), or no marker at all (Cat.# SR20071-PA-1). Create stable cell lines for quantitation of Oct4 expression Track differentiation in live cells in real timePlease note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.Choose the right vector to monitor pluripotency markers (Pluripotency Reporters) or to measure the activity of pluripotency makers (Pluripotency Response Reporters)Pluripotency response reportersSBI’s Pluripotency Response Reporters work for both human and mouse stem cellsCatalog #Promoter/Enhancer elementsReporterSelection cassetteSR20070-PA/VA-1Oct4 CR4pGreenFire (GFP-Luc)NoneSR20070-PA-NOct4 CR4pGreenFire (GFP-Luc)EF1α-NeoSR20070-PA-POct4 CR4pGreenFire (GFP-Luc)EF1α-PuroSR20071-PA/VA-1Sox2 SRR2pGreenFire (GFP-Luc)NoneSR20071-PA-NSox2 SRR2pGreenFire (GFP-Luc)EF1α-NeoSR20071-PA/VA-PSox2 SRR2pGreenFire (GFP-Luc)EF1α-PuroPluripotency reportersCatalog #SpeciesPromoter/enhancer elementReporterSR10033PA/VA-1HumanOct-4pGreenZeoSR10043PA/VA-1HumanOct-4pRedZeoSR10053PA/VA-1HumanOct-4pRedTKSR10029PA/VA-1MouseOct-4pGreenZeoSR10045PA/VA-1MouseOct-4pRedZeoSR10054PA/VA-1MouseOct-4pRedTKSR10030PA/VA-1HumanNanogpGreenZeoSR10042PA/VA-1HumanNanogpRedZeoSR10055PA/VA-1HumanNanogpRedTKSR10031PA/VA-1MouseNanogpGreenZeoSR10044PA/VA-1MouseNanogpRedZeoSR10056PA/VA-1MouseNanogpRedTK

美国SBI代理

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

美国SBI代理

ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1