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.img-style{heigth:450px;width:450px;}Overview:Efficientexosomeisolationfromplasma“WethereforepursuedtheExoQuick®methodforfurtherstudy,asthesesamplesrequiredmuchlesssampleinput,akeybenefitwhenworkingwithclinicalsamplesandmousemodels1.” Whenisolatingexosomesfromplasma,fibrinogenandfibrincanimpedeefficientrecovery.Bypre-treatingplasmawiththrombin,thefibrinogencanbeconvertedtofibrinandeasilypre-clearedbyasimplecentrifugationstep.Theresultingserum-likesolutioncannowbetreatedwithExoQuick,originalformulation,forquantitativeexosomeisolationthatiscompatIBLewithhigh-throughputmethods.TheExoQuickPlasmaPrepwithThrombinKitincludesbothExoQuickandthrombinforefficientexosomeisolationfromplasma.ExoQuick’sfast,ultracentrifugation-freemethod:SavestimeandlaborIseasilyscalableConservesprecioussampleDelivershighyieldsoffunctional,highqualityexosomesCanbeusedtoisolateexosomesforawiderangeofdownstreamapplications,includingBioMarkerstudiesExosomalmiRNAprofilingExosomalproteomicsExosomallipidomics/metabolomicsFunctionalstudies,suchasincell-to-cellsignalingBasicBIOLOGy,suchasroleintumOrigenesisExoQuickisaproprietarypolymerthatgentlyprecipitatesexosomes.Forexosomeisolationfromplasmayoucanfirstdefibrinateyoursamplewiththrombin,andthencontinuewiththestandardExoQuickprotocolofpre-clearingyoursampleofcellsandcellulardebris,addingtheappropriateamountofExoQuick,refrigerating,andthencentrifuging(seetheproductmanualforprotocoldetails).Yourexosomeswillbeinthepellet,readyforresUSPensioninanappropriatesolution.BiofluidSamplevolumeExoQuick-TCVolumeTissueculturemedia,urine,cerebrospinalfluid(CSF),etc.5mL1mLInelectronmicroscopystudies,exosomesisolatedwithExoQuickappearsimilartoexosomesisolatedusingultracentrifugation1-2,andtheseexosomesarealsoactiveinnumerousfunctionalassays1-3.ExosomesisolatedwithExoQuickcanbeusedforalltypesofproteinprofilingandproteincharacterizationstudies,suchasmassspectrometry,Westernblotting,ELISA,andmore.HigherproteinyieldsareachievedbyExoQuickpurificationthanbychromatography,DynaBeads,orultracentrifugation.ExosomesisolatedwithExoQuickalsoprovideexcellentsamplesforstudyingexosome-associatednucleicacidssuchasmicroRNAs,siRNAs,andevenmRNA.QuantitativeanalyticaltechniquessuchasqPCR,microarraystudies,andnext-generationsequencingareallcompatiblewithnucleicacidsisolatedfromExoQuick-purifiedexosomes.Backedbyagrowingnumberofpublications,ExoQuickisoftenthebestoptionforresearchersworkingwithlowsamplevolumes,suchasclinicalresearchsamplesorsmallanimalmodels.ExoQuickexosomeisolationmethodsarepatentedtechnologies4.ChoosetherightExoQuickforyourbiofluid:Catalog#ProductBiofluidEXOQ5A-1,EXOQ20A-1TheOriginalExoQuickForserum,plasma,andascitesfluidEXOTC10A-1,EXOTC50A-1ExoQuick-TC®ForallotherbiofluidsEXOLP5A-1ExoQuick-LPForremovingcontaminatinglipoproteinparticlesfromplasmaorserumbeforeExoQuickprecipitationEXOQ5TM-1ExoQuickPlasmaPrepwithThrombinForde-fibrinatingplasmabeforeexosomeisolationforefficientrecoveryandhighyieldsEXOCG50A-1ExoQuick-CGForpreparingexosomesusedinpre-clinicalinvivoapplicationsEQPL10A-1,EQPL10TCExoQuickPLUSandExoQuick-TCPLUSForsensitiveapplicationssuchasmassspectrometry,exosomelabeling,andinvivo/exvivoexosomedeliveryREFERENCESChughPE,etal.SystemicallyCirculatingViralandTumor-DerivedMicroRNAsinKSHV-AssociatedMalignancies.PLoSPathog.2013.9(7):e1003484.PMCID:PMC3715412.UmezuT,etal.LeukemiacelltoendothelialcellcommunicationviaexosomalmiRNAs.Oncogene.2013May30.32(22):2747-55.PMID:22797057.SohelMM,etal.ExosomalandNon-ExosomalTransportofExtra-CellularmicroRNAsinFollicularFluid:ImplicationsforBovineOocyteDevelopmentalCompetence.PLoSOne.2013Nov4.8(11):e78505.PMCID:PMC3817212.AntesT,etal.MethodsforMicrovesicleIsolationandSelectiveRemoval.PatentNo.:US9,005,888B2.HowItWorks:High-throughput,quantitativeexosomerecoveryfromplasmaExoQuickPlasmaPrepwithThrombincanbeusedtopurifyexosomesfromplasma1.Withasimpleworkflowinvolvingminimalhands-ontimeandlowinputsamplevolumerequirements,ExoQuickisanexcellentoptionforresearcherswhoneedtopurifymultipleexosomesamplesand/orsamplesfromsmallanimalmodelsorclinicalresearchsamples.Toisolateexosomesfromplasmasimply:IncubatewiththrombintoconvertfibrinogentofibrinClearfibrin,cells,andcelldebriswithaquicklow-speedspinAddanappropriatevolumeofExoQuicktoaslittleas100µlsampleIncubateforatleastonehourat4°CIsolateexosomeswitha30-minutelow-speedspin(1500g).Isolatedexosomescanbefoundinthepelletandresuspendedinanappropriatesolution.Youcanverifythepresenceofexosomeswithanumberofdifferentmethods,includingWesternblottingforgeneralexosomemarkers(CD63,CD9,CD81,andHSP70),NanoSightanalysis,orEM(learnaboutdifferentwaystodetectexosomesandmoreinourExosomeBasicsGuide).TheBottomLineWithExoQuick,youcanobtainhigh-qualityexosomesfrommostbiofluidsusingaprotocolthatcaneasilybeperformedonmultiplesamplesandrequiresverylowvolumesofinputsample.REFERENCESChughPE,etal.SystemicallyCirculatingViralandTumor-DerivedMicroRNAsinKSHV-AssociatedMalignancies.PLoSPathog.2013.9(7):e1003484.PMCID:PMC3715412.
美国SBI代理
System Biosciences,简称SBI,美国加州湾区新成立的生技公司,致力于独特、创新生物技术之开发,以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰(RNAi)研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术,为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司,提供基于慢病毒
美国SBI代理
ExoQuick and ExoQuick-TC Products
Product | Size | Catalog # |
ExoQuick serum exosome precipitation solution (5 ml) | 75 reactions | EXOQ5A-1 |
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product | 75 reactions | EXOQ5TM-1 |
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product | 100 reactions | TMEXO-1 |
ExoQuick serum exosome precipitation solution (20 ml) | 300 reactions | EXOQ20A-1 |
ExoQuick-TC for Tissue Culture Media and Urine (10ml) | 10 reactions | EXOTC10A-1 |
ExoQuick-TC for Tissue Culture Media and Urine (50ml) | 50 reactions | EXOTC50A-1 |
Exosome-depleted FBS Media Supplement - USA Certified | 50 ml | EXO-FBS-50A-1 |
Exosome-depleted FBS Media Supplement - USA Certified | 250 ml | EXO-FBS-250A-1 |
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit | 5 reactions | EXOLP5A-1 |
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文章排行榜
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Bpsbioscience/Boc-D-FMK/27611-1/5 mg
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Cisbio全国独家代理商
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Nbsbio/SAMPLE APPLICATION BASKET FOR 5.0 CM ID COLUMN/124268/1 Ea
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Nbsbio/SEMI-MICRO CELLS, SET OF 5/132342/1 Ea
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Nbsbio/Sodium hydroxide/SB0617.SIZE.500G/1 Ea
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Nbsbio/ORGANIC CHROMATOGRAPHY COLUMN, 15 CM ID X 75 CM LONG/125062/1 Ea
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Nbsbio/All-In-One DNA/RNA/Protein Miniprep Kit/BS88003.SIZE.50preps/1 Ea
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