.img-style{heigth:450px;width:450px;}Overview:QuickandefficientexosomeisolationforsensitiveapplicationsWhiletheExoQuick®familyofreagentsspeed-upandsimplifyexosomeisolationandarecompatIBLewithmostdownstreamuses,someapplicationsareespeciallysensitivetocarry-overofnon-exosomalproteins—applicationssuchasmassspectrometry,WesternblottingoflowabundanceMarkers,exosomelabeling,andinvivo/exvivoexosomedelivery.WhichiswhySBIoffersExoQuickPLUS.Lessismore—ExoQuickPLUSandExoQuick-TCPLUSforwhenyouneedanextralevelofconfidence:Better—deliversasmuchas150-foldhigheryieldsthanultracentrifugation-basedmethodsCleaner—dramaticallyreducestheamountofcarry-overproteincomparedtoothercolumn-orprecipitation-basedkits,andevenultracentrifugation-basedmethodsPowerful—detectsbiomarkerswithasmuchasa3-foldincreaseinsensitivityFast—takeslessthan10minutesofhands-on-timeafterinitialExoQuickisolationBuildingonourpopularExoQuickfamilyofproducts,ExoQuick-TCPLUSdeliversdramaticallyreducedalbumincontaminationinserum/plasmapreparationscomparedtoutracentrifugation-basedisolationmethods,andconsiderablylesscarryoverproteincomparedtootherexosomeisolationkits.Evenbetter,isolationwiththeExoQuickPLUSfamilyofproductsresultsinmuchhigheryieldsthanisolationwithultracentrifugation—asmuchas150-foldhigher.Withasimpletreatmentofourready-to-use,customizedExoQuickPLUSMicrospheres(kitscontainenoughExoQuick-TCandpre-packedMicrospheresfor10reactions*),youcanobtainhighlypure,intactexosomessuitableforavarietyofprotein-sensitiveapplications.Totalhands-ontimeafterExoQuick-TCisolationislessthan10minutes!*Forexosomeisolationfromserumorplasma,onereactionisdefinedas250µLofserumorplasma.Forexosomeisolationfromtissueculturemedia,onereactionisdefinedas5mLofmedia.LearnmoreaboutExoQuick—clickhere.ChoosetherightExoQuickforyourbiofluid:Catalog#ProductBiofluidEXOQ5A-1,EXOQ20A-1TheOriginalExoQuickForserum,plasma,andascitesfluidEXOTC10A-1,EXOTC50A-1ExoQuick-TC®ForallotherbiofluidsEXOLP5A-1ExoQuick-LPForremovingcontaminatinglipoproteinparticlesfromplasmaorserumbeforeExoQuickprecipitationEXOQ5TM-1ExoQuickPlasmaPrepwithThrombinForde-fibrinatingplasmabeforeexosomeisolationforefficientrecoveryandhighyieldsEXOCG50A-1ExoQuick-CGForpreparingexosomesusedinpre-clinicalinvivoapplicationsEQPL10A-1,EQPL10TCExoQuickPLUSandExoQuick-TCPLUSForsensitiveapplicationssuchasmassspectrometry,exosomelabeling,andinvivo/exvivoexosomedeliveryChoosebetweenExoQuick/ExoQuick-TCandExoQuickPLUS/ExoQuick-TCPLUSbasedonyourapplicationExoQuickExoQuick-TCExoQuickPLUSExoQuick-TCPLUSProteinDetectionWesternblottingforgeneralexosomemarkers(e.g.CD9,CD63,CD81,TSG101,Alix)••••••••••High-sensitivityWesternblotting(e.g.lowabundancebiomarkers)••••••••qPCRAnalysisqPCRofcodingandnon-codingRNAs(e.g.mRNA,miRNA,andlncRNA)••••••••••High-throughputBiomarkerDiscoveryRNA-seqofexosomalRNAs••••••••••Massspectrometryofexosomalproteins••••••••Lipidomics/metabolomicsofexosomalcargo••••••••ExosomeLabeling••••••••Invivo/exvivoExosomeDelivery•••••••••••••Highlyrecommended,•NotrecommendedHowItWorks:High-throughput,quantitativeexosomerecoveryExoQuick-TCPLUScanbeusedtopurifyexosomesfromawidevarietyofbiofluids,includingsaliva1,urine2,follicularfluid3,tissueculturemedia4,andbreastmilk5.Withasimpleworkflowinvolvingminimalhands-ontimeandlowinputsamplevolumerequirements,ExoQuick-TCPLUSisanexcellentoptionforresearcherswhoneedtopurifymultipleexosomesamplesand/orsamplesfromsmallanimalmodelsorclinicalresearchsamplesthatwillthenbeusedinsensitivedownstreamapplications.Toisolateexosomesfromclearedserum,plasma,orascitesfluid,simply:AddanappropriatevolumeofExoQuick-TCPLUStoaslittleas100µlsampleIncubateforatleastonehourat4°CPelletexosomeswitha30-minutelow-speedspin(1500g)ResUSPendpellet,addExoQuickPLUSMicrospheres,andincubatefor10minutesat4°CRemovemicrosophereswitha30-minutelow-speedspin(1500g)ExoQuick-TCPLUS-isolatedexosomescanbefoundinthesupernatant.Youcanverifythepresenceofexosomeswithanumberofdifferentmethods,includingWesternblottingforgeneralexosomemarkers(CD63,CD9,CD81,andHSP70),NanoSightanalysis,orEM(learnaboutdifferentwaystodetectexosomesandmoreinourExosomeBasicsGuide).REFERENCESYangJ,etal.DetectionofTumorCell-SpecificmRNAandProteininExosome-LikeMicrovesiclesfromBloodandSaliva.PLoSONE.2014.9(11):e110641.PMCID:PMC4232306.AlvarezML.IsolationofurinaryexosomesforRNAbiomarkerdiscoveryusingasimple,fast,andhighlyscalablemethod.MethodsMolBiol.2014.1182:145-70.PMID:25055908.SohelMM,etal.ExosomalandNon-ExosomalTransportofExtra-CellularmicroRNAsinFollicularFluid:ImplicationsforBovineOocyteDevelopmentalCompetence.PLoSOne.2013Nov4.8(11):e78505.PMCID:PMC3817212.ZhuL,etal.Novelmethodforextractingexosomesofhepatocellularcarcinomacells.WorldJGastroenterol.2014June7.20(21):6651-6657.PMCID:PMC4047354.GuY,etal.Lactation-RelatedMicroRNAExpressionProfilesofPorcineBreastMilkExosomes.PLoSONE.2012.7(8):e43691.PMCID:PMC3427246.

美国SBI代理

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

美国SBI代理

ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1