.img-style{heigth:450px;width:450px;}Overview:Abetterwaytoisolateexosomes“WethereforepursuedtheExoQuick®methodforfurtherstudy,asthesesamplesrequiredmuchlesssampleinput,akeybenefitwhenworkingwithclinicalsamplesandmousemodels1.” Needexosomes?SBI’sExoQuick-TCenableshigh-throughput,quantitativeisolationofexosomesfromlowvolumes(aslittleas1ml)ofmostbiofluids(forserum,plasma,orascitesfluid,usetheoriginalExoQuickformulation(EXOQ5A-1orEXOQ20A-1)).CompatIBLewithawidevarietyofdownstreamapplications,ExoQuick-TCisaneffectiveandprovenalternativetoultracentrifugation1-3.ExoQuick-TC’sfast,ultracentrifugation-freemethod:SavestimeandlaborIseasilyscalableConservesprecioussampleDelivershighyieldsoffunctional,highqualityexosomesCanbeusedtoisolateexosomesforawiderangeofdownstreamapplications,includingBioMarkerstudiesExosomalmiRNAprofilingExosomalproteomicsExosomallipidomics/metabolomicsFunctionalstudies,suchasincell-to-cellsignalingBasicBIOLOGy,suchasroleintumOrigenesisExoQuick-TCisaproprietarypolymerthatgentlyprecipitatesexosomes.First,pre-clearyoursamplesofcellsandcellulardebris,andthensimplyaddtheappropriateamountofExoQuick-TCtoyourclearedbiofluid,refrigerate,andcentrifuge(seetheproductmanualforprotocoldetails).Yourexosomeswillbeinthepellet,readyforresUSPensioninanappropriatesolution.BiofluidSamplevolumeExoQuick-TCVolumeTissueculturemedia,urine,cerebrospinalfluid(CSF),etc.5mL1mLInelectronmicroscopystudies,exosomesisolatedwithExoQuick-TCappearsimilartoexosomesisolatedusingultracentrifugation1-2,andtheseexosomesarealsoactiveinnumerousfunctionalassays1-3.ExosomesisolatedwithExoQuick-TCcanbeusedforalltypesofproteinprofilingandproteincharacterizationstudies,suchasmassspectrometry,Westernblotting,ELISA,andmore.HigherproteinyieldsareachievedbyExoQuickpurificationthanbychromatography,DynaBeads,orultracentrifugation.ExosomesisolatedwithExoQuick-TCalsoprovideexcellentsamplesforstudyingexosome-associatednucleicacidssuchasmicroRNAs,siRNAs,andevenmRNA.QuantitativeanalyticaltechniquessuchasqPCR,microarraystudies,andnext-generationsequencingareallcompatiblewithnucleicacidsisolatedfromExoQuick-TC-purifiedexosomes.Backedbyagrowingnumberofpublications,ExoQuick-TCisoftenthebestoptionforresearchersworkingwithlowsamplevolumes,suchasclinicalresearchsamplesorsmallanimalmodels.ExoQuick-TCexosomeisolationmethodsarepatentedtechnologies4.ChoosetherightExoQuickforyourbiofluid:Catalog#ProductBiofluidEXOQ5A-1,EXOQ20A-1TheOriginalExoQuickForserum,plasma,andascitesfluidEXOTC10A-1,EXOTC50A-1ExoQuick-TC®ForallotherbiofluidsEXOLP5A-1ExoQuick-LPForremovingcontaminatinglipoproteinparticlesfromplasmaorserumbeforeExoQuickprecipitationEXOQ5TM-1ExoQuickPlasmaPrepwithThrombinForde-fibrinatingplasmabeforeexosomeisolationforefficientrecoveryandhighyieldsEXOCG50A-1ExoQuick-CGForpreparingexosomesusedinpre-clinicalinvivoapplicationsEQPL10A-1,EQPL10TCExoQuickPLUSandExoQuick-TCPLUSForsensitiveapplicationssuchasmassspectrometry,exosomelabeling,andinvivo/exvivoexosomedeliveryChoosebetweenExoQuick/ExoQuick-TCandExoQuickPLUS/ExoQuick-TCPLUSbasedonyourapplicationExoQuickExoQuick-TCExoQuickPLUSExoQuick-TCPLUSProteinDetectionWesternblottingforgeneralexosomemarkers(e.g.CD9,CD63,CD81,TSG101,Alix)••••••••••High-sensitivityWesternblotting(e.g.lowabundancebiomarkers)••••••••qPCRAnalysisqPCRofcodingandnon-codingRNAs(e.g.mRNA,miRNA,andlncRNA)••••••••••High-throughputBiomarkerDiscoveryRNA-seqofexosomalRNAs••••••••••Massspectrometryofexosomalproteins••••••••Lipidomics/metabolomicsofexosomalcargo••••••••ExosomeLabeling••••••••Invivo/exvivoExosomeDelivery•••••••••••••Highlyrecommended,•NotrecommendedREFERENCESChughPE,etal.SystemicallyCirculatingViralandTumor-DerivedMicroRNAsinKSHV-AssociatedMalignancies.PLoSPathog.2013.9(7):e1003484.PMCID:PMC3715412.UmezuT,etal.LeukemiacelltoendothelialcellcommunicationviaexosomalmiRNAs.Oncogene.2013May30.32(22):2747-55.PMID:22797057.SohelMM,etal.ExosomalandNon-ExosomalTransportofExtra-CellularmicroRNAsinFollicularFluid:ImplicationsforBovineOocyteDevelopmentalCompetence.PLoSOne.2013Nov4.8(11):e78505.PMCID:PMC3817212.AntesT,etal.MethodsforMicrovesicleIsolationandSelectiveRemoval.PatentNo.:US9,005,888B2.HowItWorks:High-throughput,quantitativeexosomerecoveryExoQuick-TCcanbeusedtopurifyexosomesfromawidevarietyofbiofluids,includingsaliva1,urine2,follicularfluid3,tissueculturemedia4,andbreastmilk5.Withasimpleworkflowinvolvingminimalhands-ontimeandlowinputsamplevolumerequirements,ExoQuick-TCisanexcellentoptionforresearcherswhoneedtopurifymultipleexosomesamples.Toisolateexosomesfromanyclearedbiofluid,simply:AddanappropriatevolumeofExoQuick-TCIncubateforatleastonehourat4°CIsolateexosomeswitha30-minutelow-speedspin(1500g).Isolatedexosomescanbefoundinthepelletandresuspendedinanappropriatesolution.Youcanverifythepresenceofexosomeswithanumberofdifferentmethods,includingWesternblottingforgeneralexosomemarkers(CD63,CD9,CD81,andHSP70),NanoSightanalysis,orEM(learnaboutdifferentwaystodetectexosomesandmoreinourExosomeBasicsGuide).TheBottomLineWithExoQuick-TC,youcanobtainhigh-qualityexosomesfrommostbiofluidsusingaprotocolthatcaneasilybeperformedonmultiplesamplesandrequiresverylowvolumesofinputsample.REFERENCESYangJ,etal.DetectionofTumorCell-SpecificmRNAandProteininExosome-LikeMicrovesiclesfromBloodandSaliva.PLoSONE.2014.9(11):e110641.PMCID:PMC4232306.AlvarezML.IsolationofurinaryexosomesforRNAbiomarkerdiscoveryusingasimple,fast,andhighlyscalablemethod.MethodsMolBiol.2014.1182:145-70.PMID:25055908.SohelMM,etal.ExosomalandNon-ExosomalTransportofExtra-CellularmicroRNAsinFollicularFluid:ImplicationsforBovineOocyteDevelopmentalCompetence.PLoSOne.2013Nov4.8(11):e78505.PMCID:PMC3817212.ZhuL,etal.Novelmethodforextractingexosomesofhepatocellularcarcinomacells.WorldJGastroenterol.2014June7.20(21):6651-6657.PMCID:PMC4047354.GuY,etal.Lactation-RelatedMicroRNAExpressionProfilesofPorcineBreastMilkExosomes.PLoSONE.2012.7(8):e43691.PMCID:PMC3427246.Citations:Jiang,S,etal.(2017)RoleofmicroRNA-130binplacentalPGC-1α/TFAMmitochondrialbiogenesispathway.Biochem.Biophys.Res.Commun..2017Jun3;487(3):607-612.PMID:28433632Galazka,G,etal.(2017)Multiplesclerosis:Serum-derivedexosomesexpressmyelinproteins.Mult.Scler..2017Feb1;:1352458517696597.PMID:28273783Gholizadeh,S,etal.(2017)Microfluidicapproachesforisolation,detection,andcharacterizationofextracellularvesicles:Currentstatusandfuturedirections.BiosensBioelectron.2017May15;91:588-605.PMID:28088752Szatmári,T,etal.(2017)ExtracellularVesiclesMediateRADIation-InducedSystemicBystanderSignalsintheBoneMarrowandSpleen.FrontImmunol.2017Apr11;8:347.PMID:28396668Lim,JH,etal.(2017)ComparativeanalysisofmicroRNAandmRNAexpressionprofilesincellsandexosomesundertolueneexposure.ToxicolInVitro.2017Jun1;41:92-101.PMID:28245982Slowey,PD.(2017)SalivaryDiagnosticsUsingPurifiedNucleicAcids.MethodsMol.Biol..2017Dec7;1537:3-15.PMID:27924585Fry,CS,etal.(2017)MyogenicProgenitorCellsControlExtracellularMatrixProductionbyFibroblastsduringSkeletalMuscleHypertrophy.CellStemCell.2017Jan5;20(1):56-69.PMID:27840022Chen,L,etal.(2017)Exosomesderivedfromhumanmenstrualblood-derivedstemcellsalleviatefulminanthepaticfailure.StemCellResTher.2017Jan23;8(1):9.PMID:28115012Qu,Y,etal.(2017)ExosomesderivedfrommiR-181-5p-modifiedadipose-derivedmesenchymalstemcellspreventliverfibrosisviaautophagyactivation.J.Cell.Mol.Med..2017Apr6;.PMID:28382720Maeda,Y,etal.(2017)Synovium-DerivedMicroRNAsRegulateBonePathwaysinRheumatoidArthritis.J.BoneMiner.Res..2017Mar1;32(3):461-472.PMID:27676131Umezu,T,etal.(2017)ReplenishingexosomesfromolderbonemarrowstromalcellswithmiR-340inhibitsmyeloma-relatedangiogenesis.BloodAdvances.2017May16;1(13):812-823.Link:BloodAdvancesSaha,B,etal.(2017)CirculatingandExosome-PackagedHepatitisCSingle-StrandedRNAInduceMonocyteDifferentiationviaTLR7/8toPolarizedMacrophagesandFibrocytes.J.Immunol..2017Mar1;198(5):1974-1984.PMID:28122964Hoptay,CE,Ferrante,S&Hackett,TL.(2017)AsthmaticBronchialAirwayEpithelialExosomesDoNotPromoteMesenchymalFibrosisDespiteExosomalMicroRNAPredictedTargets.Conference.;.Link:ConferenceHuang,Z,etal.(2017)UrinaryExosomalmiR-193aCanBeaPotentialBiomarkerfortheDiagnosisofPrimaryFocalSegmentalGlomerulosclerosisinChildren.BiomedResInt.2017Mar1;2017:7298160.PMID:28246603Vanni,I,etal.(2017)Exosomes:anewhorizoninlungcancer.DrugDiscov.Today.2017Jun1;22(6):927-936.PMID:28288782Bomfim,MM.(2017)EfeitosdoestresseoxidativoduranteaproduçãoinvitrodeembriõesbovinossobreomiR-199aegenesalvoERBB2eERBB3.Thesis.;.Link:ThesisVal,S,etal.(2017)PurificationandcharacterizationofmicroRNAswithinmiddleearfluidexosomes:implicationinotitismediapathophysiology.Pediatr.Res..2017Apr5;.PMID:28157838Kim,MS.(2017)Exosome-EncapsulatedWater-InsolubleSmallMoleculeChemotherapeuticsfortheTreatmentofPulmonaryMetastases.Poster.;.Link:PosterNuzhat,Z,etal.(2017)Tumour-derivedexosomesasasignatureofpancreaticcancer–liquidbiopsiesasindicatorsoftumourprogression.Oncotarget.2017Mar7;8(10):17279-17291.PMID:27999198Osawa,S,etal.(2017)Fibronectinonextracellularvesiclesfrommicrovascularendothelialcellsisinvolvedinthevesicleuptakeintooligodendrocyteprecursorcells.Biochem.Biophys.Res.Commun..2017Jun17;488(1):232-238.PMID:28499870

美国SBI代理

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

美国SBI代理

ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1