.img-style{heigth:450px;width:450px;}Overview:AvoidinadvertentlystudyingbovineexosomeswithheatinactivatedExo-FBSFetalbovineserum,orFBS,isanimportantcomponentofmanytypesofcellculturemedia,andsomeresearchersrequireheatinactivatedFBS.Butforresearchersinterestedinisolatingexosomesfromculturedcells,standardheatinactivatedFBScanintroduceunwantedcomplications—bovineexosomes,whichcancausesignificantbackgroundissuesorinterferewithfunctionalstudies.WhichiswhySBIdevelopedheatinactivatedExo-FBS,ourpatentedexosome-depletedFBS.Heatinactivatedusingahighlyquality-controlledprocessExosome-sizedvesiclesremovedVerylowlevelsofCD63-positivecowexosomesUndetectablelevelsofcowmiRNAsComparablegrowthratesasstandardFBSInterchangeablewithstandardFBS(add10%inDMEMorRPMI)SupportingData:HighqualityandgreatperformanceExo-FBShasgreatlyreducedlevelsofbovineexosomes(Figures1and2),bovinemiRNAs(Figure3),andisevencleanerthanultracentrifugedFBS(Figure4).CellgrowthinmediasupplementedwithExo-FBSissimilartocellgrowthinmediasupplementedwithstandardFBS(Figure5).Exo-FBShasgreatlyreducedlevelsofbovineexosomesFigure1.NanoSightparticleanalysisshowslowlevelsofexosomesinExo-FBS.WhilestandardFBScontainsexosome-sizedparticles(toppanels),Exo-FBSshowsalmostnoparticles(bottompanels).StandardFBSandExo-FBSsampleswerediluted1:1000andthenanalyzedforparticlesizeandabundanceusingaNanoSightLM10instrument.Figure2.Bovineα-CD63ELISAshowslowlevelsofexosomesinExo-FBSCD63isanexosome-specificMarker.Anα-CD63ELISAofstandardFBSandExo-FBSshowsverylowlevelsofCD63inExo-FBS,supportingtheNanoSightparticleanalysiswhichshowedlownumbersofexosome-sizedparticlesinExo-FBS(Figure1).Equalvolumes(50µl)ofeitherstandardFBSorExo-FBSdepletedmediasupplementwereusedandthegraphedresultsnormalizedtothesignallevelofstandardFBS.Figure3.qPCRassaysshowundetectablelevelsofbovineexosomalmiRNAsinExo-FBS.WhilestandardFBScontainsamplifiablemiRNAs(12ofthe72individualmiRNAstested,leftpanels),Exo-FBSshowsnoamplifiablemiRNAs(rightpanels).StandardFBSandExo-FBSmediasupplements(4ml)weretreatedwithTrizolextractionmethodstorecoverexosomalRNAs.RNAwasconvertedtoCDNAand72individualbovinemicroRNAsweremeasuredbyqPCRusingSBI’sQuantiMirsystem.Figure4.NanoSightanalysisshowsExo-FBSiscleanerthanultracentrifugedFBS.QualityControldataisgeneratedoneverybatchofExo-FBSproducedatSBIbycomparingNanoSightparticlecountanalysestothesourceFBS,FBSultracentrifugedfor18hours,andExo-FBS.Allsampleswerediluted1:100anddatacollectedintriplicate.Figure5.Cellsgrownin10%Exo-FBSshowcomparablegrowthratesto10%standardFBS.HT1080fibrosarcomacells,PC-3prostatecancercells,MCF-7breastcancercells,andHEK293cellswereseededateither10,000or20,000cellsandthenculturedunderstandardconditionsat37°Cwith5%CO2for5days.Citations:Rody,WJ,etal.(2017)Theuseofcellcultureplatformstoidentifynovelmarkersofboneanddentinresorption.OrthodCraniofacRes.2017Jun1;:89-94.PMID:28643914Beltrami,C,etal.(2017)HumanPericardialFluidContainsExosomesEnrichedwithCardiovascular-ExpressedMicroRNAsandPromotesTherapeuticAngiogenesis.Mol.Ther..2017Mar1;25(3):679-693.PMID:28159509Sun,L,etal.(2017)Exosomesderivedfromhumanumbilicalcordmesenchymalstemcellsprotectagainstcisplatin-inducedovariangranulosacellstressandapoptosisinvitro.SciRep.2017May31;7(1):2552.PMID:28566720Zhou,X,etal.(2017)ExosomeproductionanditsregulationofEGFRduringwoundhealinginrenaltubularcells.Am.J.Physiol.RenalPhysiol..2017Mar29;:ajprenal.00078.2017.PMID:28356285Josson,S,Gururajan,M&WKChung,L.(2017)miRNACharacterizationfromtheExtracellularVesicles.bio-protocol.;7(4).Link:bio-protocolMcNicholas,K&Michael,MZ.(2017)Immuno-characterizationofExosomesUsingNanoparticleTrackingAnalysis.MethodsMol.Biol..2017Dec12;1545:35-42.PMID:27943205Nakamura,K,etal.(2017)ExosomesPromoteOvarianCancerCellInvasionthroughTransferofCD44toPeritonealMesothelialCells.Mol.CancerRes..2017Jan1;15(1):78-92.PMID:27758876Banton,S.(2017)Humanperipheralreticulocyteisolationandexosomereleaseinvitro.Thesis.;.Link:ThesisOh,HJ,etal.(2017)Convectiveexosome-tracingmicrofluidicsforanalysisofcell-non-autonomousneurogenesis.Biomaterials.2017Jan1;112:82-94.PMID:27750100Devhare,PB,etal.(2017)Exosome-MediatedIntercellularCommunicationbetweenHepatitisCVirus-InfectedHepatocytesandHepaticStellateCells..J.Virol..2017Mar15;91(6).PMID:28077652Huang,JH,etal.(2017)SystemicAdmiNISTrationofExosomesReleasedfromMesenchymalStromalCellsAttenuatesApoptosis,InflammationandPromotesAngiogenesisafterContusionSpinalCordInjuryinRats.J.Neurotrauma.2017Jun30;.PMID:28665182Harischandra,DS,etal.(2017)Environmentalneurotoxicantmanganeseregulatesexosome-mediatedextracellularmiRNAsincellculturemodelofParkinson’sdisease:Relevancetoα-synucleinmisfoldinginmetalneurotoxicity.Neurotoxicology.2017Apr24;.PMID:28450057Crookenden,MA,etal.(2017)EffectofcirculatingexosomesfromtransitioncowsonMADIn-Darbybovinekidneycellfunction.J.DairySci..2017Jul1;100(7):5687-5700.PMID:28456398Philley,JV,etal.(2017)Exosomesecretomeandmediatedsignalinginbreastcancerpatientswithnontuberculousmycobacterialdisease.Oncotarget.2017Mar14;8(11):18070-18081.PMID:28160560Rashed,MH,etal.(2017)ExosomalmiR-940maintainsSRC-mediatedoncogenicactivityincancercells:apossIBLeroleforexosomaldisposaloftumorsuppressormiRNAs.Oncotarget.2017Mar21;8(12):20145-20164.PMID:28423620Huang,MB,etal.(2017)Secretionmodificationregion-derivedpeptideblocksexosomereleaseandmediatescellcyclearrestinbreastcancercells.Oncotarget.2017Feb14;8(7):11302-11315.PMID:28076321Lambrecht,J,etal.(2017)CirculatingECV-AssociatedmiRNAsasPotentialClinicalBiomarkersinEarlyStageHBVandHCVInducedLiverFibrosis.FrontPharmacol.2017Feb24;8:56.PMID:28232800Xin,H,etal.(2017)SecondaryReleaseofExosomesFromAstrocytesContributestotheIncreaseinNeuralPlasticityandImprovementofFunctionalRecoveryAfterStrokeinRatsTreatedWithExosomesHarvestedFromMicroRNA133b-OverexpressingMultipotentMesenchymalStromalCells.CellTransplant.2017Feb16;26(2):243-257.PMID:27677799Ye,W,etal.(2017)Plasma-derivedexosomescontributetoinflammationviatheTLR9-NF-κBpathwayinchronicheartfailurepatients.Mol.Immunol..2017Jul1;87:114-121.PMID:28433888Hosseinkhani,B,etal.(2017)Directdetectionofnano-scaleextracellularvesiclesderivedfrominflammation-triggeredendothelialcellsusingsurfaceplasmonresonance.Nanomedicine.2017Jul1;13(5):1663-1671.PMID:28366819

美国SBI代理

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

美国SBI代理

ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1