.img-style{heigth:450px;width:450px;}Overview:GowiththeflowforeasyexosomeisolationusingFACSTheCD63Exo-FlowCaptureKithasallthereagentsyouneedtopurifyexosomesusingFACS—magneticstreptavidinbeads,CD63-biotincaptureantibody,washandelutionbuffers,andreversIBLeExo-FITCstain(Cat.#EXOFLOW800A-1)—yousupplytheFACSandtheoptionalmagneticstand(Cat.#EXOFLOW700A-1).Ourwell-validatedCD63-biotinantibodyandhigh-qualitykitcomponentsensurereliable,reproducibleexosomepurificationbasedonthepresenceofCD63ontheexosomesurface.Andwithourlarger-than-typicalbeadsize(9.1μmdiameter)exosomecaptureishighlyefficient,assistingwithrecoveryofrareexosomepopulations.NotethatwealsoofferreversibleExo-APC(Cat.#Exo-FLOW810A-1)asanalternativestaintoExo-FITC.Arangeofwell-validatedantibodiesforreliableandreproduciblepurificationLarge-sizedmagneticbeadsincreasetheefficiencyofexosomecaptureReversibleExo-FITCandExo-APCstainscanbecompletelyremovedafterFACSExosomeElutionBuffersimultaneouslyremovesExo-FITC(orExo-APC)andelutesexosomesfromthemagneticbeadsfordownstreamapplicationssuchasfunctionalstudiesMorethanjustCD63sTofacilitatethewidestrangeofstudies,SBIbuilttheExo-Flowsystemtobehighlymodular.WeofferarangeofbiotinylatedantibodiesaswellasaBasicExo-FlowKit(Cat.#CSFLOWBASICA-1)withnoantibodysothatyoucanuseyourownbiotinylatedantibodies.Cat.#KitEXOFLOW100A-1CD9Exo-FlowCaptureKitEXOFLOW150A-1TetraspaninExo-FlowComboCaptureKitEXOFLOW200A-1CD31Exo-FlowCaptureKitEXOFLOW210A-1CD44Exo-FlowCaptureKitEXOFLOW300A-1CD63Exo-FlowCaptureKitEXOFLOW400A-1CD81Exo-FlowCaptureKitEXOFLOW500A-1Rab5bExo-FlowCaptureKitEXOFLOW600A-1HLA-GExo-FlowCaptureKitEXOFLOW610A-1CD14Exo-FlowCaptureKitEXOFLOW620A-1CD68Exo-FlowCaptureKitEXOFLOW630A-1EpCAMExo-FlowCaptureKitEXOFLOW650A-1α2-Macroglobulin(A2M)Exo-FlowCaptureKitEXOFLOW660A-1CD73Exo-FlowCaptureKitHowItWorks:EasilypurifyexosomesusingFACSwiththeCD63Exo-FlowCaptureKitAtaglanceSimply(1)coupletheCD63-biotinantibodytothemagneticstreptavidinbeads,(2)usetheCD63-coupledmagneticbeadstocaptureexosomesthathavebeenisolatedusingeitherExoQuick®orultracentrifugation,(3)washawayunboundexosomes,andthen(4)stainwithreversibleExo-FITC(excitationandemissionwavelengthsof494nmand518nm,respectively).YoursampleisnowreadyforFACSanalysis.TousethepurifiedexosomesafterFACS,addtheincludedExosomeElutionBuffertosimultaneouslyremovetheExo-FITCstainandeluteintactexosomesfromthebeads.Citations:Protocol,IIEI.(2017)ListofComponents.Product.;.Link:ProductEtzerodt,A,etal.(2017)SolubleectodomainCD163andextracellularvesicle-associatedCD163aretwodifferentlyregulatedformsof‘solubleCD163’inplasma.SciRep.2017Jan13;7:40286.PMID:28084321Chen,C,etal.(2017)Mesenchymalstemcelltransplantationintight-skinmiceidentifiesmiR-151-5pasatherapeutictargetforsystemicsclerosis.CellRes..2017Apr1;27(4):559-577.PMID:28106077Meyer,C,etal.(2017)Pseudotypingexosomesforenhancedproteindeliveryinmammaliancells..IntJNanomedicine.2017May1;12:3153-3170.PMID:28458537Domenis,R,etal.(2017)CharacterizationoftheProinflammatoryProfileofSynovialFluid-DerivedExosomesofPatientswithOsteoarthritis.MediatorsInflamm..2017Jun21;2017:4814987.PMID:28634420Smith,JA&Daniel,R.(2016)HumanvaginalfluidcontainsexosomesthathaveaninhibitoryeffectonanearlystepoftheHIV-1lifecycle.AIDS.2016Nov13;30(17):2611-2616.PMID:27536982Wen,Z,etal.(2016)NADPHoxidasedeficiencyunderliesdysfunctionofagedCD8+Tregs.J.Clin.Invest..2016May2;126(5):1953-67.PMID:27088800Junker,K,etal.(2016)ExtracellularVesiclesandTheirRoleinUrologicMalignancies..Eur.Urol..2016Aug1;70(2):323-31.PMID:26924769Raimondo,F,etal.(2016)Urinaryproteomicsforthestudyofgenetickidneydiseases..ExpertRevProteomics.2016Mar2;13(3):309-24.PMID:26698090Mukherjee,K,etal.(2016)ReversibleHuR-microRNAbindingcontrolsextracellularexportofmiR-122andaugmentsstressresponse.EMBORep..2016Aug1;17(8):1184-203.PMID:27402548Rim,KT&Kim,SJ.(2016)QuantitativeAnalysisofExosomesFromMurineLungCancerCellsbyFlowCytometry.JCancerPrev.2016Sep1;21(3):194-200.PMID:27722146Nudel,K.(2016)NeisseriagonorrhoeaemodulatesepithelialcellresponsesviatheinductionandreleaseoftheinhibitorofapoptosisproteincIAP2inexosomes.Thesis.;.Link:ThesisPeterson,MF,etal.(2015)Integratedsystemsforexosomeinvestigation.Methods.2015Oct1;87:31-45.PMID:25916618Bhattarai,N,etal.(2015)ConservedMotifswithinHepatitisCVirusEnvelope(E2)RNAandProteinIndependentlyInhibitTCellActivation.PLoSPathog..2015Sep1;11(9):e1005183.PMID:26421924Nudel,K,Massari,P&Genco,CA.(2015)NeisseriagonorrhoeaeModulatesCellDeathinHumanEndocervicalEpithelialCellsthroughExportofExosome-AssociatedcIAP2.Infect.Immun..2015Sep1;83(9):3410-7.PMID:26077759Bednarczyk,M,Biryło,M&DAWIDOWICZ,A.(2015)ModernGeodeticTechniquesinSpatialMeasurement.Thesis.;.Link:ThesisDiNoto,G,etal.(2014)ImmunoglobulinFreeLightChainsandGAGsMediateMultipleMyelomaExtracellularVesiclesUptakeandSecondaryNfκBNuclearTranslocation.FrontImmunol.2014Nov11;5:517.PMID:25386176Jia,S,etal.(2014)Emergingtechnologiesinextracellularvesicle-basedmoleculardiagnostics.ExpertRev.Mol.Diagn..2014Apr1;14(3):307-21.PMID:24575799Santana,SM,e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美国SBI代理

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

美国SBI代理

ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1