.img-style{heigth:450px;width:450px;}Overview:Streamlineaffinity-basedexosomeimmunopurificationWithmagneticbeadsalreadypre-coupledtoanti-CD81antibodiesanddeliveredina32-wellformat,SBI’sExo-Flow32CD81IPKitsimplifieshigh-throughput,antibody-basedexosomeisolation.Ourmagneticbead-coupledanti-CD81antibodiesareextensivelyvalidated,andthehigh-qualityExo-FlowIPkitcomponentsensurereliable,reproducIBLeaffinity-basedexosomepurificationdirectlyfromserumorplasma.Exosomescanalsobepurifiedfromotherbiofluidssuchasmedia,urine,andCSF,butmustfirstbeconcentratedusingeitherExoQuick-TC®orultracentrifugation.Selectionofwell-validatedantibodiesforreliableandreproduciblepurificationLarge-sizedmagneticbeadsincreasetheefficiencyofexosomecaptureAvailablein32-and96-wellformatsforhigh-throughput,affinity-basedexosomeisolationEvenbetter,withourlarger-than-typicalbeadsize(9.1μmdiameter)exosomecaptureishighlyefficient,enablingcaptureofbillionsofexosomesfromonesample.EachIPkitcomeswithallthenecessaryreagentsforimmunopurification—captureantibodypre-coupledtomagneticbeadsin96-wellclearplates(as8-wellstrips)withcovers,washbuffer,ExosomeElutionBuffer,andclearingreagent(toremoveExosomeElutionBuffer).A96-wellplatemagnet,theExo-FlowMag96,canbepurchasedseparately(Cat.#EXOFLOWMAG-1).DifferentformatsfordifferentneedsTofacilitatearangeofstudies,SBIbuilttheExo-FlowIPsystemtobehighlymodular.Weofferarangeofmagneticbead-coupledantibodiesin32-and96-wellformats.Cat.#KitEXOFLOW32A-CD63Exo-Flow32CD63IPkitEXOFLOW32A-CD81Exo-Flow32CD81IPkitEXOFLOW32A-CD9Exo-Flow32CD9IPkitEXOFLOW32A-TetraExo-Flow32TetraIPkit(CD9,CD63,CD81)EXOFLOW96A-CD63Exo-Flow96CD63IPkitEXOFLOW96A-CD81Exo-Flow96CD81IPkitEXOFLOW96A-CD9Exo-Flow96CD9IPkitEXOFLOW96A-TetraExo-Flow96TetraIPkit(CD9,CD63,CD81)SupportingData:SeeisolationdatausingExo-FLOWIPKitsForthesestudies,eitherhumanserumorHEK293exosomesconcentratedfromcellculturemediausingExoQuick-TCwereaddedtotheantibody-coupledmagneticbeads.Afterwashing,exosomeswereelutedandrecoveryestimatedusingastandardBCAproteinassay.Figure1.CD63andCD9,twoexosomeMarkers,arereADIlydetectedinsamplespurifiedusingExo-FlowKits.Approximately1µgofproteinwasloadedperwellona4-20%gradientproteinPAGE.TheproteinswereseparatedandtransferredtonitrocellulosemembranesforWesternblotanalysis.Theblotswereprobedwitheitherwithanti-CD63oranti-CD9antibodiestodetecttheexosomeproteinmarkers.Figure2.NanoSightanalysisshowsExo-FlowIPKitsdelivergoodyieldsofparticleswhosesizesareconsistentwithexosomes.Citations:Chen,C,etal.(2017)Mesenchymalstemcelltransplantationintight-skinmiceidentifiesmiR-151-5pasatherapeutictargetforsystemicsclerosis.CellRes..2017Apr1;27(4):559-577.PMID:28106077Meyer,C,etal.(2017)Pseudotypingexosomesforenhancedproteindeliveryinmammaliancells..IntJNanomedicine.2017May1;12:3153-3170.PMID:28458537Domenis,R,etal.(2017)CharacterizationoftheProinflammatoryProfileofSynovialFluid-DerivedExosomesofPatientswithOsteoarthritis.MediatorsInflamm..2017Jun21;2017:4814987.PMID:28634420Protocol,IIEI.(2017)ListofComponents.Product.;.Link:ProductEtzerodt,A,etal.(2017)SolubleectodomainCD163andextracellularvesicle-associatedCD163aretwodifferentlyregulatedformsof‘solubleCD163’inplasma.SciRep.2017Jan13;7:40286.PMID:28084321Mukherjee,K,etal.(2016)ReversibleHuR-microRNAbindingcontrolsextracellularexportofmiR-122andaugmentsstressresponse.EMBORep..2016Aug1;17(8):1184-203.PMID:27402548Rim,KT&Kim,SJ.(2016)QuantitativeAnalysisofExosomesFromMurineLungCancerCellsbyFlowCytometry.JCancerPrev.2016Sep1;21(3):194-200.PMID:27722146Nudel,K.(2016)NeisseriagonorrhoeaemodulatesepithelialcellresponsesviatheinductionandreleaseoftheinhibitorofapoptosisproteincIAP2inexosomes.Thesis.;.Link:ThesisSmith,JA&Daniel,R.(2016)HumanvaginalfluidcontainsexosomesthathaveaninhibitoryeffectonanearlystepoftheHIV-1lifecycle.AIDS.2016Nov13;30(17):2611-2616.PMID:27536982Wen,Z,etal.(2016)NADPHoxidasedeficiencyunderliesdysfunctionofagedCD8+Tregs.J.Clin.Invest..2016May2;126(5):1953-67.PMID:27088800Junker,K,etal.(2016)ExtracellularVesiclesandTheirRoleinUrologicMalignancies..Eur.Urol..2016Aug1;70(2):323-31.PMID:26924769Raimondo,F,etal.(2016)Urinaryproteomicsforthestudyofgenetickidneydiseases..ExpertRevProteomics.2016Mar2;13(3):309-24.PMID:26698090Bhattarai,N,etal.(2015)ConservedMotifswithinHepatitisCVirusEnvelope(E2)RNAandProteinIndependentlyInhibitTCellActivation.PLoSPathog..2015Sep1;11(9):e1005183.PMID:26421924Nudel,K,Massari,P&Genco,CA.(2015)NeisseriagonorrhoeaeModulatesCellDeathinHumanEndocervicalEpithelialCellsthroughExportofExosome-AssociatedcIAP2.Infect.Immun..2015Sep1;83(9):3410-7.PMID:26077759Bednarczyk,M,Biryło,M&DAWIDOWICZ,A.(2015)ModernGeodeticTechniquesinSpatialMeasurement.Thesis.;.Link:ThesisPeterson,MF,etal.(2015)Integratedsystemsforexosomeinvestigation.Methods.2015Oct1;87:31-45.PMID:25916618Basu,S&Bhattacharyya,SN.(2014)Insulin-likegrowthfactor-1preventsmiR-122productioninneighbouringcellstocurtailitsintercellulartransfertoensureproliferationofhumanhepatomacells.NucleicAcidsRes..2014Jun1;42(11):7170-85.PMID:24813441DiNoto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美国SBI代理

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

美国SBI代理

ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1