.img-style{heigth:450px;width:450px;}Overview:Confirm exosome isolation with confidence When you’re ready to confirm that you’ve isolated exosomes, stick with the exosome experts at SBI. Our polyclonal rabbit anti-FLOT1 antibodies recognize the human, mouse, and rat forms of the protein, and are a great choice for general exosome detection, especially when used in conjunction with antibodies for other exosome markers such as the tetraspanins CD9, CD63, and CD81, as well as the heat shock protein HSP70 (if you have any questions about the best antibodies to use for your exosome project, we’re ready to help! Just email us at tech@systembio.com). And, like all of our antibodies, our anti-FLOT1 antibody and the included goat anti-rabbit HRP secondary antibodies are rigorously validated for exosome detection via Western blotting. Rigorously validated Hand-picked selection Expert exosome support Choose the right antibody for your exosome detection needs Cat.# Antibody to Notes Link to ExoCarta entry for the human protein General exosome markers EXOAB-CD9A-1 CD9 Tetraspanin ExoCarta_928 EXOAB-CD63A-1 CD63 Tetraspanin ExoCarta_967 EXOAB-CD81A-1 CD81 Tetraspanin ExoCarta_93185 EXOAB-Hsp70A-1 HSP70 ExoCarta_3303, ExoCarta_3304, ExoCarta_3306, ExoCarta_3308, ExoCarta 3310, ExoCarta_3311 EXOAB-ALIX-1 ALIX Programmed cell death 6 interacting protein (PDCD6IP) ExoCarta_10015 EXOAB-ANXA5-1 ANXA5-1 Annexin A5 ExoCarta_308 EXOAB-TSG101-1 TSG101 Tumor susceptibility gene 101 ExoCarta_7251 EXOAB-FLOT1-1 FLOT-1 Flotillin-1 ExoCarta_10211 Tissue-specific exosome markers EXOAB-CXCR4-1 CXCR4 Specific for B-cells, T-cells, and platelets ExoCarta_7852 EXOAB-EPCAM1-1 EpCam Specific for cancer-derived exosomes ExoCarta_4072 EXOAB-VMTN-1 Vimentin Specific for mesenchymal stem cells ExoCarta_7431 Supporting Data: See isolation data using Exo-FLOW IP Kits For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay. Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4-20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers. Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.

美国SBI代理

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

美国SBI代理

ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1