.img-style{heigth:450px;width:450px;}Overview:EasilytrackexosomeswithaCD81-GFPfusionMonitorexosomedynamicswiththepCT-CD81-GFP(pCMV)ExosomeCyto-Tracer(alsoavailableasaCD81-RFPfusion,pCT-CD81-RFP).Providedaseitherlentivectororpre-packagedvirus,thisCyto-TracerfusesGFPtothetetraspaninCD81,whichthengetspackagedintoexosomesproducedbythetransfectedcells.GreatforcreatingstablecelllinesthatproduceGFP-labeledexosomesaswellaslabelingexosomesfromprimarycells,tumorcells,stemcells,andmore.Stablelentivector-basedsystemIdealforco-localizationstudiesMonitorexosomedynamicsandfunctionalstudiesinrealtimeLabelexosomesfromprimarycells,tumorcells,stemcells,andmoreChoosetheexosomequantitationmethodthat’sbestforyourstudiesPromoter(Cat.#)Cyto-TracerTargetLocationPeptideTagCMV(CYTO100-PA-1/CYTO100-VA-1)MSCV(CYTO100-PB-1/CYTO100-VB-1)pCT-Mem-GFPPlasmaMembraneNeuromodulinCMV(CYTO101-PA-1/CYTO101-VA-1)MSCV(CYTO101-PB-1/CYTO101-VB-1)pCT-InnerMem-GFPInnerLeafletofMembraneFarnesylationsignalCMV(CYTO102-PA-1/CYTO102-VA-1)MSCV(CYTO102-PB-1/CYTO102-VB-1)pCT-Mito-GFPMitochondriaCytochromeCoxidaseVIIICMV(CYTO103-PA-1/CYTO103-VA-1)pCT-ER-GFPEndoplasmicReticulumERtargetingconsensusCMV(CYTO104-PA-1/CYTO104-VA-1)pCT-Golgi-GFPGolgiApparatusGalactosyltrasferaseCMV(CYTO105-PA-1/CYTO105-VA-1)pCT-Secretory-GFPER-Golgi VesiclesSecretoryconsensuspeptideCMV(CYTO106-PA-1/CYTO106-VA-1)pCT-Lyso-GFPLysosomeCathepsinBCMV(CYTO107-PA-1/CYTO107-VA-1)pCT-Endo-GFPEndosomeRhoBCMV(CYTO107R-PA-1/CYTO107R-VA-1)pCT-Endo-RFPEndosomeRhoBCMV(CYTO108-PA-1/CYTO108-VA-1)MSCV(CYTO108-PB-1/CYTO108-VB-1)pCT-Pero-GFPPeroxisomePeroxisomalconsensussignalCMV(CYTO120-PA-1/CYTO120-VA-1)pCT-Autophago-GFPAutophagosomeLC3CMV(CYTO120R-PA-1/CYTO120R-VA-1)pCT-Autophago-RFPAutophagosomeLC3CMV(CYTO109-PA-1/CYTO109-VA-1)pCT-CD63-GFPExosome/SecretoryCD63TetraspaninCMV(CYTO109R-PA-1/CYTO109R-VA-1)pCT-CD63-RFPExosome/SecretoryCD63TetraspaninCMV(CYTO122-PA-1/CYTO122-VA-1)pCT-CD9-GFPExosome/SecretoryCD9TetraspaninCMV(CYTO123-PA-1/CYTO123-VA-1)pCT-CD9-RFPExosome/SecretoryCD9TetraspaninCMV(CYTO124-PA-1/CYTO124-VA-1)pCT-CD81-GFPExosome/SecretoryCD81TetraspaninCMV(CYTO125-PA-1/CYTO125-VA-1)pCT-CD81-RFPExosome/SecretoryCD81TetraspaninCMV(CYTO110-PA-1/CYTO110-VA-1)MSCV(CYTO110-PB-1/CYTO110-VB-1)pCT-Actin-GFPCytoskeletonβ-actinCMV(CYTO111-PA-1/CYTO111-VA-1)MSCV(CYTO111-PB-1/CYTO111-VB-1)pCT-Tublin-GFPCytoskeletonα-tublinCMV(CYTO111R-PA-1/CYTO111R-VA-1)pCT-Tublin-RFPCytoskeletonα-tublinCMV(CYTO112-PA-1/CYTO112-VA-1)pCT-MAP4-GFPMicrotubuleMicrotubuleassociatedprotein4(MAP4)CMV(CYTO113-PA-1/CYTO113-VA-1)MSCV(CYTO113-PB-1/CYTO113-VB-1)pCT-H2B-GFPNucleusH2BCMV(CYTO114-PA-1/CYTO114-VA-1)pCT-Apoptosis-Luc Caspase3/7activationCyclicinactiveluciferaseCMV(CYTO115-PA-1/CYTO115-VA-1)pCT-GFP-BAXCytosoltoMitochondriaBAXCMV(CYTO116-PA-1/CYTO116-VA-1)pCT-Catenin-GFPCytosoltoNucleus/CellMembraneβ-CateninactivationCMV(CYTO117-PA-1/CYTO117-VA-1)pCT-CMV-PSD95-GFPDendriteMembranesPSD-95CMV(CYTO118-PA-1/CYTO118-VA-1)MSCV(CYTO118-PB-1/CYTO118-VB-1)pCT-Cyto-GFPCytosolicCompartmentGFP-UntaggedCMV(CYTO119-PA-1/CYTO119-VA-1)pCT-Cyto-RFPCytosolicCompartmentRFP-UntaggedMSCV(CYTO121-PB-1/CYTO121-VB-1)pCT-Cyto-GFpLucGFPandLuciferaseControlGFPandLuciferase(Untagged)SupportingData:SeesomeofourexosomeCyto-TracersinactionThefollowingfigureandvideosarefrom:GarciaNA,etal.GlucoseStarvationinCardiomyocytesEnhancesExosomeSecretionandPromotesAngiogenesisinEndothelialCells.PLoSONE.2015.10(9).PMCID:PMC4578916.Figure4fromGarcia,etal.ExosometransferfromCMstoECs.(A)H9c2transfectedwithpCT-CD63-GFP.FACSanalysisof90%GFPpositivecells.(B)Representativeimagesfromconfocaltime-lapsemicroscopyofmouseECs(ACTB-DsRed)(EC;red)co-culturedwithH9C2-CD63-GFPcells(green),previouslyculturedfor24hin+/-Stmedium.ExosometransferfromH9C2CMstoECcanbeobserved(S1andS2Movies,below).WhitearrowsshowCD63-GFPstructuresinsideECs(C)RepresentativeimmunostainingofH9C2-CD63-GFPandHUVECco-cultures;anti-GFP(green)andanti-CD31(red).TheimagesillustrateGFPfluorescencefromCD63-GFPexosomesinCD31-positivecells(red)after24hincubationin+/-Stmedium.WhitearrowsshowCD63-GFPstructuresinsideECs.Labeledexosomes.(Toppanels)CD63-GFPCyto-Tracerstransfectedintoahumanfibrosarcomacellline.(Bottompanels)CD9-GFPandCD9-RFPCyto-Tracersco-transfectedintoHEK293cells.Citations:Braganza,A,etal.(2017)UBE3BIsaCalmodulin-regulated,Mitochondrion-associatedE3UbiquitinLigase.J.Biol.Chem..2017Feb10;292(6):2470-2484.PMID:28003368Gonzalez-King,H,etal.(2017)HypoxiaInducIBLeFactor-1αPotentiatesJagged1-MediatedAngiogenesisbyMesenchymalStemCell-DerivedExosomes.StemCells.2017Jul1;35(7):1747-1759.PMID:28376567Jawhari,S,etal.(2017)AutophagyandTrkC/NT-3signalingjoinedforcesboostthehypoxicglioblastomacellsurvival.Carcinogenesis.2017Jun1;38(6):592-603.PMID:28402394Soares,VYR.(2017)OpapeldasvesículasextracelularesnafisiopatologiadaperdaauditivaocasionadapeloSchwannomavestibular.Thesis.;.Link:ThesisYoon,C,etal.(2016)DeliveryofmiR-155toretinalpigmentepithelialcellsmediatedbyBurkitt’slymphomaexosomes.TumourBiol..2016Jan1;37(1):313-21.PMID:26211004Jawhari,S.(2015)Etudedessignalisationsautophagiqueetneurotrophiquedansdeslignéesdeglioblastomehumainactivéeslorsdel’hypoxie.Thesis.;.Link:ThesisLoCicero,A,etal.(2015)Exosomesreleasedbykeratinocytesmodulatemelanocytepigmentation.NatCommun.2015Jun24;6:7506.PMID:26103923VARLET,P,PU-PH,P&DJAVAHERI-MERGNY,M.(2015)SOHAJAWHARI.Thesis.;.Link:ThesisLiang,Y.(2015)Exosome/MicrovesicleCargoContentsRecruitmentinaGlycosylation-dependentPathway.Thesis.;.Link:ThesisSato,Y,etal.(2014)MTCL1crosslinksandstABIlizesnon-centrosomalmicrotubulesontheGolgimembrane.NatCommun.2014Nov4;5:5266.PMID:25366663Liang,Y,etal.(2014)ComplexN-linkedglycansserveasadeterminantforexosome/microvesiclecargorecruitment.J.Biol.Chem..2014Nov21;289(47):32526-37.PMID:25261472Fang,Q,etal.(2014)HSP90regulatesDNArepairviatheinteractionbetweenXRCC1andDNApolymeraseβ.NatCommun.2014Nov26;5:5513.PMID:25423885

美国SBI代理

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

美国SBI代理

ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1