.img-style{heigth:450px;width:450px;}Overview:LabelexosomeswithGFPusingXPackTMEasilycreatestablecelllinesthatproduceGFP-labeledexosomeswiththeXPackCMV-XP-GFP-EF1α-PuroExpressionLentivector.TakeadvantageofSBI’swell-provenlentivectortechnologytoexpressGFPfromthestrongCMVpromoter(alsoavailablewithanMSCVpromoterforexpressioninhematopoieticandstemcells)andtheoptimizedXPackexosometargetingtagtopackageGFPintoexosomes.TheXPackSystemataglance:Powerful—enablesawealthofapplications,includingExosomelabelingExosomecargotrackingExosomesascargodeliveryvehiclesExosomesasbiotherapeuticsExosome-generatingstablecelllineswithproteincargo-of-interestOptimized—SBIdevelopedtheXPackpeptidetagforrobustexosometargetingReliable—basedonSBI’shighly-cited,well-provenlentivectortechnologyVersatileTwopromoteroptionsforexpressioninawiderangeofcellsComesaspre-builtreporters(GFP,RFP,luciferase)orready-to-customizecloninglentivectorsChoosetheXPackLentivectorthat’srightforyou CMVPromoterFormostcelllinesMSCVPromoterForhematopoieticandstemcelllines  Customizewithourcloningvector XPAK510PA-1XPackCMV-XP-MCS-EF1α-PuroXPAK710PA-1XPackMSCV-XP-MCS-EF1α-PuroLabelexosomeswithpre-builtreportersGFPXPAK530CL-1XPAK530PA-1XPAK530VA-1XPackCMV-XP-GFP-EF1α-PuroXPAK730PA-1XPAK730VA-1XPackMSCV-XP-GFP-EF1α-PuroRFPXPAK531PA-1XPackCMV-XP-RFP-EF1α-PuroXPAK731PA-1XPackMSCV-XP-RFP-EF1α-PuroLuciferaseXPAK532CL-1XPAK532PA-1XPAK532VA-1XPackCMV-XP-Luciferase-EF1α-PuroXPAK732PA-1XPAK732VA-1XPackMSCV-XP-Luciferase-EF1α-PuroSupportingData:XPackefficientlypackagesGFPintoexosomeswhichcanbeusedtodeliverGFPtotargetcellsPart1:PackagingGFPintoexosomeswithXPackTheXPack-GFPfusionproteinisefficientlypackagedasexosomecargoanddeliveredtotargetcells.HEK-293cellsweretransfectedwithaplasmidencodinganN-terminalXPack-GFPfusionprotein(Cat.#XPAK530PA-1).Twenty-fourhourslater,GFPlocalizationpatternswerevisualizedonaLeicaDMI300Bfluorescentmicroscope(Figure1,upperpanel).GFPexpressionpatternswithintransfectedcellsdemonstratealocalizationpatterncharacteristicofexosomalloADIng,withpunctatespotsalongthecellperipheryindicatingbuddingvesiclesandabrightlyfluorescentspotinthecellinteriorcorrespondingtothemultivesicularbody(MVB),thesiteofexosomeassemblyandmaturationpriortosecretion.After48hours,exosomeswereisolatedusingExoQuick-TC®andanalyzedforGFPviaWesternblot(Figure1,lowerpanel).Equalamountsofexosomallysates(15µg)wereseparatedongradientSDSPAGEgelsanddetectedforGFPproteinexpression.Thecontrolforexosomeloadingwasananti-CETPantibody.XPack-GFPexosomeshadveryhighamountsofGFPproteinloadedascargo.Figure1.XPackefficientlypackagesGFPintoexosomes.(Upperpanel)XPack-GFPlocalizationduringpackaging.(Lowerpanel)WesternblotshowingrobustloadingofGFPintotheisolatedexosomes.Part2:XPack-GFPExosomeDeliverytoTargetCellsTheXPack-GFPexosomesisolatedasdescribedabovewerethenaddedtorecipientHEK-293cellstotestforGFPdelivery.CellsexposedtoXPack-GFPexosomeswereimagedbyfluorescentmicroscopy,whichshowedsubcellularGFPlocalizationconsistentwithendocytosisoftheXPack-GFPexosomesanddeliveryoftheXPack-GFPproteincargointothecell(Figure2,leftpanels).Subsequently,theserecipientcellswerewashedtwicewithPBSandcellularlysateswerepreparedforWesternblotanalysisusinganti-GFPantibodies.Anti-GAPDHwasusedasacellularproteinloadingcontrol.TheWesternblotdatafurtherverifiedthesuccessfuldeliveryofGFPproteinstorecipientcellsviatheXPackexosomeengineeringtechnology(Figure2,rightpanel).Figure2.XPack-GFPexosomesdeliverGFPtotargetcells.(Leftpanel)XPack-GFPlocalizationduringpackaging.(Rightpanel)WesternblotshowingrobustGFPdeliverytotargetcells.

美国SBI代理

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

美国SBI代理

ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1