.img-style{heigth:450px;width:450px;}Overview:Label exosomes with luciferase using XPackTM Easily create stable cell lines that produce luciferase-labeled exosomes with the XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivector. Take advantage of SBI’s well-proven lentivector technology to express luciferase from the strong CMV promoter (also available with an MSCV promoter for expression in hematopoietic and stem cells) and the optimized XPack exosome targeting tag to package luciferase into exosomes. The XPack System at a glance: Powerful—enables a wealth of applications, including Exosome labeling Exosome cargo tracking Exosomes as cargo delivery vehicles Exosomes as biotherapeutics Exosome-generating stable cell lines with protein cargo-of-interest Optimized—SBI developed the XPack peptide tag for robust exosome targeting Reliable—based on SBI’s highly-cited, well-proven lentivector technology Versatile Two promoter options for expression in a wide range of cells Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors Choose the XPack Lentivector that’s right for you   CMV Promoter For most cell lines MSCV Promoter For hematopoietic and stem cell lines     Customize with our cloning vector   XPAK510PA-1 XPack CMV-XP-MCS-EF1α-Puro XPAK710PA-1 XPack MSCV-XP-MCS-EF1α-Puro Label exosomes with pre-built reporters GFP XPAK530CL-1 XPAK530PA-1 XPAK530VA-1 XPack CMV-XP-GFP-EF1α-Puro XPAK730PA-1 XPAK730VA-1 XPack MSCV-XP-GFP-EF1α-Puro RFP XPAK531PA-1 XPack CMV-XP-RFP-EF1α-Puro XPAK731PA-1 XPack MSCV-XP-RFP-EF1α-Puro Luciferase XPAK532CL-1 XPAK532PA-1 XPAK532VA-1 XPack CMV-XP-Luciferase-EF1α-Puro XPAK732PA-1 XPAK732VA-1 XPack MSCV-XP-Luciferase-EF1α-Puro Supporting Data: XPack efficiently packages luciferase into exosomes which can be used to deliver GFP to target cells Part 1: Packaging luciferase into exosomes with XPack The XPack-Luciferase fusion protein is efficiently packaged as exosome cargo and delivered to target cells. HEK-293 cells were transfected with a plasmid encoding an N-terminal XPack-Luciferase fusion protein (Cat.# XPAK532PA-1). Forty-eight hours later, exosomes were isolated using ExoQuick-TC® and luciferase levels quantified and compared to exosomes isolated from untransfected cells (Figure 1). Only XPack-Luciferase exosomes show robust luminescence indicating efficient packaging of luciferase into exosomes. Figure 1. XPack efficiently packages luciferase into exosomes. Part 2: XPack-Luciferase Exosome Delivery to Target Cells The XPack-Luciferase exosomes isolated as described above were then added to recipient HEK-293 cells to test for luciferase delivery. After 24-hours, robust luminescence from cells exposed to XPack-Luciferase exosomes shows delivery of the luciferase cargo to the target cells (Figure 2). In contrast, untreated cells or cells treated with exosomes from untransfected cells show no luminescence. Figure 2. XPack-Luciferase exosomes deliver luciferase to target cells.

美国SBI代理

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

美国SBI代理

ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1