A better way to isolate exosomes"We therefore pursued the ExoQuick® method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models1."Need exosomes? SBI"s ExoQuick-TC enables high-throughput, quantitative isolation of exosomes from low volumes (as little as 1 ml) of tissue culture media and certain biofluids (saliva, urine, follicular fluid, and breast milk). Note, to isolate exosomes from serum, plasma, or ascites fluid, use the original ExoQuick formulation (EXOQ5A-1 or EXOQ20A-1). Compatible with a wide variety of downstream applications, ExoQuick-TC is an effective and proven alternative to ultracentrifugation1-3.ExoQuick-TC’s fast, ultracentrifugation-free method:Saves time and laborIs easily scalableConserves precious sampleDelivers high yields of functional, high quality exosomesCan be used to isolate exosomes for a wide range of downstream applications, includingBiomarker studiesExosomal miRNA profilingExosomal proteomicsExosomal lipidomics/metabolomicsFunctional studies, such as in cell-to-cell signalingBasic biology, such as role in tumorigenesisExoQuick-TC is a proprietary polymer that gently precipitates exosomes. First, pre-clear your samples of cells and cellular debris, and then simply add the appropriate amount of ExoQuick-TC to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.BiofluidSample volumeExoQuick-TC VolumeTissue culture media, urine, cerebrospinal fluid (CSF), etc.5 mL1 mLIn electron microscopy studies, exosomes isolated with ExoQuick-TC appear similar to exosomes isolated using ultracentrifugation1-2, and these exosomes are also active in numerous functional assays1-3.Exosomes isolated with ExoQuick-TC can be used for all types of protein profiling and protein characterization studies, such as mass spectrometry, Western blotting, ELISA, and more. Higher protein yields are achieved by ExoQuick purification than by chromatography, DynaBeads, or ultracentrifugation.Exosomes isolated with ExoQuick-TC also provide excellent samples for studying exosome-associated nucleic acids such as microRNAs, siRNAs, and even mRNA. Quantitative analytical techniques such as qPCR, microarray studies, and next-generation sequencing are all compatible with nucleic acids isolated from ExoQuick-TC-purified exosomes.Backed by a growing number of publications, ExoQuick-TC is often the best option for researchers working with low sample volumes, such as clinical research samples or small animal models.ExoQuick-TC exosome isolation methods are patented technologies4.Choose the right ExoQuick for your application:ApplicationProductCatalog #Purest EV isolationExoQuick ULTRA andExoQuick-TC ULTRAEQULTRA-20A-1 EQULTRA-20TC-1 General purpose EV isolationExoQuick andExoQuick-TCEXOQ20A-1 EXOTC50A-1 EV isolation for pre-clinical/in vivo studiesExoQuick-CGEXOCG50A-1EV isolation that removes contaminating lipoprotein particles from plasma or serumExoQuick-LPEXOLP5A-1EV isolation that includes a de-fibrinating plasma step prior to isolationExoQuick Plasma Prep with ThrombinEXOQ5TM-1REFERENCESChugh PE, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog. 2013. 9(7): e1003484. PMCID: PMC3715412.Umezu T, et al. Leukemia cell to endothelial cell communication via exosomal miRNAs. Oncogene. 2013 May 30. 32(22):2747-55. PMID: 22797057.Sohel MM, et al. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence. PLoS One. 2013 Nov 4. 8(11):e78505. PMCID: PMC3817212.Antes T, et al. Methods for Microvesicle Isolation and Selective Removal. Patent No.: US 9,005,888 B2.

美国SBI代理

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

美国SBI代理

ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1