RNAandDNAFromOneSampleBestyieldandqualityforbothRNA&DNASuperiortranscriptomestABIlity&fidelityShipsampleswithoutcoldchainProveninhigh-throughputworkflowsProductPreserveRNAandDNAInstantlyTheRNAgardBloodSystemisdesignedforthecollection,preservationandpurificationofRNAandDNAfromwholebloodsamples.TheRNAgardBloodpreservativereagentinthetubesrapidlypermeatesandlysescellularstructuresandmembranestostabilizeandprotectRNAandDNAfromdegradation.Preservationiseffectivefor14daysatroomtemperatureor1monthat4°C.ProductComparisonProperties*RNAgardCompetitorPRNAYieldRNAQuality(RIN)TranscriptomeStability**RNA&DNAfromSameTube(High-Throughput)*Samplestestedafter2weeksat25°C,7Weeksat4°C**byIlluminaHumanHT-12v4arraywith>47KtranscriptsSaveSaveSaveSaveSaveSaveSaveSaveSaveSaveDataProtectRNAinstantlyatambienttemperaturesFigure1.GeneexpressionanalysisofbloodRNApreservationafter3and14daysinRNAgardBlood(RGB).Humanbloodfrom2healthydonorswascollectedinRNAgardtubesorcompetitor’sRNApreservationtubes.RNAwasisolatedoncollectiondayandafter3or14daysofroomtemperaturestorage.Top:Representativeanalysis,forDonor2,ofgeneexpressionprofileofmorethan47000transcripts,usingtheIluminaHumanHT12BeadArray,forRNAextractedfrombloodcollectedinRNAgardBloodorcompetitor’sTubesafter3days(A,C)or14days(B,D)ofroomtemperaturestorage,relativetoRNAextractedfromfreshlycollectedblood,usingRNAisolationmethodsrecommendedforeachproduct.Bottom:Tablesummarizingthemicroarrayresults.Displayedarethenumberofgeneswith>2-foldchangeinexpressionlevelsrelativetoexpressionlevelsonthedayofbloodsamplecollection.RNAyieldfromBioMaxiBloodRNAPurificationKitFigure2.RNAyieldanalyzedbyUVspectroscopy.RNAamountsareforwholetube(2.5mLblood)isolatingusingBioMaxiBloodRNAPurificationkitafter14daysofstorageatambienttemperature.14-dayRNAprotectionatambienttemperaturesFigure3.MultipledonorcomparisonofRNAgardBloodTubes(RGB)versusCompetitorP(P)after14daysofambienttemperaturestorage.200ngofRNAisvisualizedona1.2%agarosegel.BioMaxiBloodRNAPurificationkitwasusedforisolatingRNAfromRNAgardBloodTubes.RNAgardBloodSystem:ReproducIBLeandRepeatableRNAPurification:RNAPurity(A260/A280)for3Operators(A,B,C)Figure4.RNApurityobtainedusingtheRNAgardBloodSystemishighlyreproducible.Bloodwascollectedfrom8healthydonors.After3,7or15daysofsamplestorage,RNAwaspurifiedfromduplicatesamples.RNApurityofeachindividualsamplewasdeterminedbyUVspectrophotometry(A260/A280).OnDay3,duplicatesamplesforeachdonorwerepurifiedby3differentoperatorsshowingreproducibilityofoursystem.StabilityofspecificRNAtranscriptsisolatedwithRNAgardBloodSystemFigure5.RNAgardBloodTubemaintainsunalteredgeneexpressionfor14daysat18-25°C.BloodfromtwohealthydonorswascollectedinRNAgardtubesandpurifiedwithBioMaxiRNAaftertheindicatedstoragetime.GeneexpressionofC-Fos(A)andIL1-β(B),normalizedto18SrRNA,wasdeterminedbyqPCRattheindicatedstoragetime,relativetoday0samples.RNAStabilityafter7weeksat4°CFigure6.RNAyieldofwholebloodfrom5differentdonorswastestedusingtheRNAgardBloodSystemfollowingstoragefor7weeksat4°Cversuscontrol(Day=0).DatashowsthatthequalityandyieldofRNAfollowing7weeksofstorageinRNAgardBloodTubesat4°CwereequivalenttothatofRNApurifiedfromwholebloodonthedayofblooddraw.Impactoffreeze/thawconditionsontheintegrityofRNAgardBloodTubesTable1.RNAgardBloodTubesremainintactafterfreezerstoragefor7days.BloodwascollectedfromtwohealthydonorsintoRNAgardBloodTubes.Thetubeswerethenkeptatroomtemperatureforatleast2hoursbeforefreezingdown.Fourdifferentfreezingmethodsweretested,andthetubeswerestoredfrozenasindicatedfor7days.Thetubeswerethawedusingthreedifferentmethods.Notubedamages(cracks,splits,etc.)wereobserved.Stablegeneexpression>47,000transcriptsTestedonIlluminaHumanHT-12v4ArrayTestedonIlluminaHumanHT-12v4ArrayGeneTranscript:BCR-ABLFunctionalTest:RT-qPCRFunctionaltestingwithageneexpression-basedcancerdiagnostictest.RNAyield(70%higherwithRNAgard)RNAQualityRNAIntegritySaveSaveSave