Collect,ShipandStoreTissues&CellsatAmbientTemperatureProtectDNAintissues,cellsandbiopsymaterialfromdegradationatroomtemperatureCompatIBLewithmostdownstreamapplications,e.g.,PCR,qPCRandsequencing.ReducesstorageandshippingcostsandimproveslabspacemanagementOverviewDNAgard™isdesignedforimmediatestABIlizationofDNAfromintactcellsandtissueswiththeconvenienceofroomtemperatureshipping,processingandstorage.TheliquidstoragereagentrapidlypermeatescellmembranestostabilizeandprotectgenomicDNA.DNAgardWorkflowOverviewForResearchUseOnly.Notforuseindiagnosticprocedures.SaveSaveProductDataDNAprotectioninratkidneytissuesatroomtemperatureFigure1:GelimageshowinggenomicDNAisolatedfromratkidney(25mg)storedfor195daysinDNAgard,water(non-protected:NP)orfrozenat-80°Cwasanalyzedon0.8%agarosegel.(M=1kbladder).DNAgardTissueprotectsDNAinavarietyoftissuesFigure2:GelimagesshowinggenomicDNAisolatedfromavarietyofrattissues(25mg)storedfor70daysinDNAgardTissue,water(non-protected:NP)orfrozenat-80°Cwasanalyzedona0.8%agarosegel.(M=1kbladder).DNAgardprotectsDNAinmammaliancellsatroomtemperatureFigure3:(A)106293Tcellswerepelletedandthesupernatant(media)wasremoved.CellpelletswereresUSPendedin500µlofDNAgardorwater(non-protected).Forcomparison,106cellswerespottedonWhatmanFTApaperanddried.Asapositivecontrol,106cellswerestoredat–80°Cin10%DMSO.DNAgard,Whatmanandnon-protectedsampleswerestoredatroomtemperature.After84daysofincubationsampleswereprocessedforDNAisolationusingacommerciallyavailablecolumnpurificationtechnology.(TheDNAwaselutedfromtheWhatmanFTAsamplesusingWhatman’spublishedprotocol).TheDNAyieldandintegrityfrom5x104293Tcellswascompareddirectlyona0.8%agarosegel.(T’0′isDNArecoveredatthetimecellswereharvested;M=1kbladder).(B)RepresentativeDNAyieldsfromDNAgardsamplescomparedtofrozencontrols.1DNAwasisolatedfromtissuefragmentsor106mammalian293TcellsstoredinliquidDNAgardfor2months.DNAyieldwasquantifiedusingtheQuant-iTPicoGreenfluorescentassay(Invitrogen).YieldispresentedasngofDNApermgtissuefortheanimaltissuesamplesortotalDNAforthe293Tcellsamples.BroadrangeofapplicationsSuccessfulreal-timePCRandlong-rangePCRafter2monthsstorageinDNAgardandat-80°CFigure4:Ratkidneyfragmentswereweighed(approx.25mgeach),immediatelysubmergedinDNAgardsolutionorwater(NP),andstoredatroomtemperature.Controlsampleswerefrozenandstoredat-80°C.After71days,totalDNAwasrecoveredfromthestoredsamplesusingacommerciallyavailablecolumnpurificationtechnology.DNAwasanalyzedbyrealtimePCRamplificationofthebeta-actingene.SaveSaveSpecificationsProductformats:100mLbottle,CustomvolumesApplicationvolume:Atleast100µLper10mgtissue;100µLper106cellsDNAstorage:6monthsDNAstabilizationtestingtemperature:20°CUsage:ForResearchUseOnly.Notforuseindiagnosticprocedures.DownloadsClicktoexpandeachcategory&subcategory.DNAgardTissueLoADIng....accordion-groups>.panel-group>.panel:not(.panel-default)>.panel-heading>a{font-size:11pt;line-height:20px;padding:5px!important}.accordion-groups .panel:not(.panel-default)>.panel-heading>a{color:#fff;font-family:Montserrat,"segoe ui",serif}.accordion-groups .btn-lg{padding:17px 25px}img.acc-thumb{max-width:48px;height:auto!important;margin-right:10px;border-radius:3px!important}ul.list-group li{margin-left:0!important}ul.list-group{margin:0!important;padding:0!important}#accordion-groups-1 .list-group-item:empty{display:none}#accordion-groups-1 blockquote{margin:0!important}.accordion-groups .panel-default small{font-size:11px}#accordion-groups-1 .wpdm-hideme,#accordion-groups-1 .panel .panel-dnagard-tissue{display:none}