DescriptionProductAvailABIlity:InStock(deliverywithin2-3businessdays,noextrachargefordrop-shipping).Ordering:ToplaceanorderviacreditcardorPOuseAddtoCartbutton(creatinganaccountisnotrequired).PEG-LiposomeInVivoTransfectionKitsiRNA,DNA andprotein invivodeliveryreagentModesofadmiNISTration:Systemicintravenous(i.v.)injectionDirectintratumoral(i.t.)injectionAltogen’sPEG-Liposome InVivoTransfectionReagentPEGylatedliposomebasedreagentNodetectabletoxicityorinflammatoryresponseduetobiodegradablePEGmodificationComplexesarestableinserumforatleast16hEfficientdeliverytothespleen,kidney,pancreas,liver,andmultipletumortypesviasystemicadministrationEfficientdeliveryofnegativelychargedmoleculesincludingsiRNA(shRNA,microRNA),plasmidDNA,andproteinFunctionallytestedinmiceApplicableforplasmidDNA/siRNA(shRNA)co-injectionDownloadPEG-Liposomeinvivotransfectionprotocol:[PDF][Word]DownloadPowerPointpresentationforPEG-Liposome invivotransfectionkit:[PPT]DevelopedandmanufacturedbyAltogenBiosystemsDATAFigure1.Systemicadministration(i.v.)ofLiposome-PEGbasedInVivoreagentconjugatedwithsiRNAtargetingLaminA/CmRNAornon-silencingcontrolsiRNAfollowingtherecommendedprotocol.TissueswerecollectedandRNAisolated48hoursafterfirstinjection.SampleswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.RibosomalRNAlevelswereusedtonormalizetheLaminA/Cdata.Dataaremeans±SD(n=6).Figure2.Intratumoraladministration(i.t.)ofLiposome-PEGbasedInVivoreagentconjugatedwithsiRNAtargetingLaminA/CmRNAornon-silencingcontrolsiRNAfollowingtherecommendedprotocol.TissueswerecollectedandRNAisolated48hoursafterfirstinjection.SampleswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.RibosomalRNAlevelswereusedtonormalizetheLaminA/Cdata.Dataaremeans±SD(n=6).Figure3. Systemicadministration(i.v.)ofPEG-LiposomeInVivoTransfectionReagentconjugatedwith80ugofchemicallymodifiedsiRNAtargetingLaminA/CmRNAornon-silencingcontrolsiRNAfollowingtherecommendedprotocol.Tissues(Liver,Brain,Muscle,Kidney,Tumor,Heart,Spleen,Lung)werecollectedandtotalproteinfractionisolated72hoursafterfirstinjection.SampleswereanalyzedbyWesternBlotAnalysisforLaminA/Cgeneexpressionlevels.Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]Diabetologia.201255(7):2069-79.Thep47phox-andNADPHoxidaseorganiser1…Younetal [PDF]BritishJournalofCancer.2012 107(3):516-26.TIGARinducesp53-mediatedcell-cycle…Madanetal[PDF]Hypertension.201463(2):353-61.Tissuetransglutaminasecontributesto…Liuetal[PDF]Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]JounalofBIOLOGicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]Gastroenterology.2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]PLoSPathog.201410(10)ExosomesfromhepatitisCinfectedpatientstransmitHCV…Bukongetal[PDF]RequestaQuoteforPEG-LiposomeInVivoTransfectionReagentAltogenResearchServices:AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).VolumeOptions:0.5ml–10injections(Catalog#5040)1.5ml–30injections(Catalog#5041)8.0ml–160injections(Catalog#5042)
Altogen Biosystems全国代理部分产品列表:
货号
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产品
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规格
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价格(美元)
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5030
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|
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1703
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A204 Transfection Reagent (Rhabdomyosarcoma)
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0.5ml
|
170
|
1704
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A204 Transfection Reagent (Rhabdomyosarcoma)
|
1.5ml
|
348
窗体顶端
窗体底端
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2101
|
A204 Transfection Reagent (Rhabdomyosarcoma)
|
1.5ml CRISPR
|
548
窗体顶端
窗体底端
|
7018
|
A204 Transfection Reagent (Rhabdomyosarcoma)
|
8.0ml
|
1044
窗体顶端
窗体底端
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1167
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A375 Cells Transfection Reagent (CRL-1619)
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0.5ml
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195
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1168
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A375 Cells Transfection Reagent (CRL-1620)
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1.5ml
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427
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2102
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A375 Cells Transfection Reagent (CRL-1621)
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1.5ml CRISPR
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627
|
1169
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A375 Cells Transfection Reagent (CRL-1622)
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8.0ml
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1281
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5030
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Nanoparticle In Vivo Transfection Reagent
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0.5 ml - 10 injections
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445
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5010
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LIPID In Vivo Transfection Reagent
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0.5 ml - 10 injections
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405
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5040
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PEG-Liposome In Vivo Transfection Reagent
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0.5 ml - 10 injections
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465
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5020
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POLYMER In Vivo Transfection Reagent
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0.5 ml - 10 injections
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425
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5050
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Pancreas In Vivo Transfection Kit
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0.5 ml - 10 injections
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495
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5060
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Liver In Vivo Transfection Kit
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0.5 ml - 10 injections
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495
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5070
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Kidney In Vivo Transfection Kit
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0.5 ml - 10 injections
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495
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1721
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BMS2 Transfection Reagent (Bone Marrow Cells)
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0.5 ml
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231
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6345
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C6 Transfection Reagent (Glioma Cells, CCL-107)
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0.5 ml
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199
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6803
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DLD-1 Transfection Reagent (Colon Cancer, CCL-221)
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0.5 ml
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201
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1775
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HUH-7 Transfection Reagent (Liver Cancer Cells)
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0.5 ml
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179
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