DescriptionProduct Availability: In Stock (delivery within 2-3 business days, no extra charge for drop-shipping).Ordering: To place an order via credit card or PO use Add to Cart button (creating an account is not required).Transfection Reagent for PANC-1 Cells (Non-endocrine Pancreatic Cancer)Proprietary cationic lipids formulationHigh transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNAA proven reagent for establishing stable cell linesOptimized transfection protocols are adapted for use with both standard & reverse transfection methodsDownload in vitro PANC-1 transfection protocol: [PDF]Download PANC-1 CRISPR/Cas9 transfection protocol: [PDF]Download PowerPoint presentation for PANC-1 cells transfection kit: [PPT]Developed and manufactured by Altogen BiosystemsTransfection Efficiency:Reagent exhibits at least 85% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.Transfection Protocol and MSDS:Download Altogen Biosystems PANC-1 Transfection Protocol: [PDF]Download MSDS: [PDF]PANC-1 Cell Line:According to Nature, pancreatic cancer has a 5-year survival rate of 5%, making the quest for a reliable cure urgent. Pancreatic ductal adenocarcinoma is the fourth primary cause of cancer-related fatalities, comprising nearly 90% of all pancreatic malignancies. PANC-1 is a well characterized and extensively studied cell line that is frequently employed as in vitro model for pancreatic ductal adenocarcinoma research. The PANC-1 cell line plays an essential role in pancreatic cancer therapeutic assays. The PANC-1 cell line was established from pancreatic duct tissue of a 56-year-old Caucasian male with epithelioid carcinoma. PANC is short for pancreas, referring to the Human pancreas, where cancer can be found. Also, the PANC-1 cell line serves as a suitable transfection host. The growth of these cells is inhibited by L-asparaginase.PANC-1 cells are used as an in vitro model of non-endocrine pancreatic cancer for tumorigenicity studies. These cells possess the type B phenotype for glucose-6-phosphate dehydrogenase G6PD and overexpress heregulin/human epidermal growth factor receptor 2 (HER2/neu) oncogene, which is present in 60 to 70% of human pancreatic carcinomas but are estrogen receptor (ER) negative. PANC cells have been shown to be negative for MUC4 and positive for Smad4, a TGF beta signaling cascade protein inactivated in human gastrointestinal cancers. PANC can be induced to differentiate into hormone-producing islet-like clusters following stimulation by the FGF2 growth factor. The PANC-1 cell line has an estimated doubling time of 52 hours with a modal chromosome number of 63. Also, these cells have Glucose-6-phosphate dehydrogenase activity of the slow mobility of B type. Altogen Biosystems provides proprietary cationic liposome formulation that demonstrate high efficacy transfection of PANC-1 cells. Transfection kit is preoptimized for the intracellular delivery of plasmid DNA, siRNA, and mRNA molecules into PANC-1 non-endocrine pancreatic cancer cell line.Data:Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the PANC-1 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=3).Figure 2. Protein expression of Cyclophilin B in PANC-1 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into PANC-1 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.Request a Quote for PANC1 Transfection KitAltogen Biosystems: Altogen Biosystems provides preoptimized transfection products for life science research applications. Transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery to liver, pancreas, and kidney tissues), and broad range in vivo delivery reagents (PEG-Liposome, Nanoparticle-based, Lipid-based, and Polymer-based kits). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, RNA, and any other negatively charged molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.Altogen Labs Research Services:Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com). Volume Options:0.5 ml (Catalog #3225)1.5 ml (Catalog #3226)1.5 ml CRISPR (Catalog #2187)8.0 ml (Catalog #7076)

Altogen Biosystems全国代理部分产品列表：

货号	产品	规格	价格（美元）
5030
1703	A204 Transfection Reagent (Rhabdomyosarcoma)	0.5ml	170
1704	A204 Transfection Reagent (Rhabdomyosarcoma)	1.5ml	348 窗体顶端 窗体底端
2101	A204 Transfection Reagent (Rhabdomyosarcoma)	1.5ml CRISPR	548 窗体顶端 窗体底端
7018	A204 Transfection Reagent (Rhabdomyosarcoma)	8.0ml	1044 窗体顶端 窗体底端
1167	A375 Cells Transfection Reagent (CRL-1619)	0.5ml	195
1168	A375 Cells Transfection Reagent (CRL-1620)	1.5ml	427
2102	A375 Cells Transfection Reagent (CRL-1621)	1.5ml CRISPR	627
1169	A375 Cells Transfection Reagent (CRL-1622)	8.0ml	1281
5030	Nanoparticle In Vivo Transfection Reagent	0.5 ml - 10 injections	445
5010	LIPID In Vivo Transfection Reagent	0.5 ml - 10 injections	405
5040	PEG-Liposome In Vivo Transfection Reagent	0.5 ml - 10 injections	465
5020	POLYMER In Vivo Transfection Reagent	0.5 ml - 10 injections	425
5050	Pancreas In Vivo Transfection Kit	0.5 ml - 10 injections	495
5060	Liver In Vivo Transfection Kit	0.5 ml - 10 injections	495
5070	Kidney In Vivo Transfection Kit	0.5 ml - 10 injections	495
1721	BMS2 Transfection Reagent (Bone Marrow Cells)	0.5 ml	231
6345	C6 Transfection Reagent (Glioma Cells, CCL-107)	0.5 ml	199
6803	DLD-1 Transfection Reagent (Colon Cancer, CCL-221)	0.5 ml	201
1775	HUH-7 Transfection Reagent (Liver Cancer Cells)	0.5 ml	179