DescriptionProductAvailABIlity:InStock(deliverywithin2-3businessdays,noextrachargefordrop-shipping).Ordering:ToplaceanorderviacreditcardorPOuseAddtoCartbutton(creatinganaccountisnotrequired).TransfectionReagentfor293T/17Cells(EmbryonicKidneyCells,CRL-11268)ProprietarycationiclipidsformulationHightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNAEffectiveandrobustintracellulardeliveryKitincludesTransfectionEnhancerreagentProducesconsistentresults,lot-to-lot,plate-to-plate,andwell-to-wellWork inthepresenceofserumAprovenreagentforestablishingstablecelllinesOptimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethodsDownloadinvitro293T/17transfectionprotocol:[PDF]Download 293T/17CRISPR/Cas9transfectionprotocol:[PDF]DownloadPowerPointpresentationfor293T cellstransfectionkit:[PPT]DevelopedandmanufacturedbyAltogenBiosystemsTransfectionEfficiency:Reagentexhibitsatleast90%transfectionefficiencyofsiRNAdelivery.Transfectionefficiencywasdeterminedbyreal-timeRT-PCR.TransfectionProtocolandMSDS:DownloadAltogenBiosystems293TTransfectionProtocol:[PDF]DownloadMSDS:[PDF]293T/17CellLine:KidneycanceristhetwelfthleADIngcauseofdeathinoncologypatients,withmorethan338,000newcasesdiagnosedannually.ItisreportedlyresponsIBLeforover13,000fatalitieseveryyearintheUnitedStatesalone.Thelifetimeriskofdevelopingrenalcancerishigherinblackmales,asstatedbytheAmericanCancerSociety(ACS).Preclinicalcelllinemodelscouldhelpscientistsfindnewtherapiesforpatientswithkidneycancer.The293T/17epithelialcelllineisderivedfromthe293/293Tcellline,originatingfromembryonickidneycells.The293T/17celllineissimilartothe293/293Tcellline.TheyallcarrytheSV40T-antigengene,whichcausesthemtoproducethesimianvirus40(SV40),allowingtheepisomalreplicationoftransfectedplasmids.Thenumberdesignatingthisas17indicatesthelinewhichwasselectedspecificallyforitshighabilitytobetransfected.Inadditiontobeinganadequatehostforkidneycancerresearch,the293T/17celllineishelpfulforstudyingkidney-relatedinfections.293T/17couldbeusefulinmanytypesofmolecularandcellBIOLOGyexperimentalresearch.AltogenBiosystemsoffershigh-efficiencytransfectionkitsforthiscellline.Data:Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRinthe293TcellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=6).Figure2.ProteinexpressionofCyclophilinBin293T/17cells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedinto293T/17cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]RequestaQuotefor293TCellsTransfectionReagentAltogenBiosystems:AltogenBiosystemsisalifesciencescompany thatoffers celltype-specificandpre-optimizedtransfection products, elecroporationkits,and invivo deliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintra-cellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.AltogenLabsResearchServices:AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).VolumeOptions:0.5ml(Catalog#1153)1.5ml(Catalog#1154)1.5mlCRISPR(Catalog#2205)8.0ml(Catalog#1155)
Altogen Biosystems全国代理部分产品列表：
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货号
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产品
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规格
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价格（美元）
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5030
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1703
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A204 Transfection Reagent (Rhabdomyosarcoma)
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0.5ml
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170
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|
1704
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A204 Transfection Reagent (Rhabdomyosarcoma)
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1.5ml
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348
窗体顶端
窗体底端
|
|
2101
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A204 Transfection Reagent (Rhabdomyosarcoma)
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1.5ml CRISPR
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548
窗体顶端
窗体底端
|
|
7018
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A204 Transfection Reagent (Rhabdomyosarcoma)
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8.0ml
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1044
窗体顶端
窗体底端
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1167
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A375 Cells Transfection Reagent (CRL-1619)
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0.5ml
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195
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1168
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A375 Cells Transfection Reagent (CRL-1620)
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1.5ml
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427
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2102
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A375 Cells Transfection Reagent (CRL-1621)
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1.5ml CRISPR
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627
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1169
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A375 Cells Transfection Reagent (CRL-1622)
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8.0ml
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1281
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5030
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Nanoparticle In Vivo Transfection Reagent
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0.5 ml - 10 injections
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445
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5010
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LIPID In Vivo Transfection Reagent
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0.5 ml - 10 injections
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405
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5040
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PEG-Liposome In Vivo Transfection Reagent
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0.5 ml - 10 injections
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465
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5020
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POLYMER In Vivo Transfection Reagent
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0.5 ml - 10 injections
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425
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5050
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Pancreas In Vivo Transfection Kit
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0.5 ml - 10 injections
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495
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5060
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Liver In Vivo Transfection Kit
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0.5 ml - 10 injections
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495
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5070
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Kidney In Vivo Transfection Kit
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0.5 ml - 10 injections
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495
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1721
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BMS2 Transfection Reagent (Bone Marrow Cells)
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0.5 ml
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231
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6345
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C6 Transfection Reagent (Glioma Cells, CCL-107)
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0.5 ml
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199
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6803
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DLD-1 Transfection Reagent (Colon Cancer, CCL-221)
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0.5 ml
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201
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1775
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HUH-7 Transfection Reagent (Liver Cancer Cells)
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0.5 ml
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179
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