DescriptionProductAvailABIlity:InStock(deliverywithin2-3businessdays,noextrachargefordrop-shipping).Ordering:ToplaceanorderviacreditcardorPOuseAddtoCartbutton(creatinganaccountisnotrequired).TransfectionReagentfor 3LLCells(LewisLungCarcinoma Cells)Ananoparticle-basedliposomeformulationTransfectionprotocolsprovidedfortransfectionofproteins,DNA,mRNA,siRNA,shRNAandmicroRNATransfectionEnhancerreagentprovidedwiththekitProducehigherlevelofrecombinantproteinexpressionwithminimaldisruptionofnormalcellfunctionGeneratephysiologicallyrelevantdatayoucantrustEffectiveforplasmidDNA/siRNAco-transfectionLowcytotoxicityDownloadinvitro3LLtransfectionprotocol:[PDF]Download 3LLCRISPR/Cas9transfectionprotocol:[PDF]DownloadPowerPointpresentationfor3LLcellstransfectionkit:[PPT]DevelopedandmanufacturedbyAltogenBiosystemsTransfectionEfficiency:Reagentexhibitsatleast73%transfectionefficiencyofsiRNAdelivery.Transfectionefficiencywasdeterminedbyreal-timeRT-PCR.TransfectionProtocolandMSDS:DownloadAltogenBiosystems3LLTransfectionProtocol:[PDF]DownloadMSDS:[PDF]3LLCellLine:Lungcancerisconsideredthemostpreventablemalignancyglobally.However,itisthedeadliestformofcancerwithveryhighmortalityratesbecauseoflackofadequatetherapiesandaggressivetumorcellgrowth.Rodentcelllinemodelsplayanessentialroleinpreclinicalresearchtostudynoveltherapeuticagentsandtreatinghumancancer.TheLLCcelllineisawell-studiedmousecancermodelthatisextensivelyemployedasatransplantablemalignancymodel.LewisLungCarcinomacells(alsoknownas3LLcellsorLLCcells)gottheirnameafterDr.MargaretR.LewisoftheWistarInstitute,whodiscoveredcarcinomain1951.Thiscelllinehasbeenknowntoshowreducedmetastaticabilitywhenexposedtoultravioletlight.Ithasalsobeenobservedthatthepresenceofcannabinoidssuppressescellgrowth.Thesecellscouldbeusefulformedicalresearchfortreatmentstocertaintypesoflungcancer,andmanyothermolecularorcellBIOLOGystudies.Accordingtotherecentdata,theproliferativeadvantageof3LLmetastaticcellsisachievedbythegeneexpressionwhichpreventstumorcellsfromescapinghostilemicroenvironmentalconditions.AltogenBiosystemsprovidesnanoparticle-basedtransfectionkitsforthiscellline.Data:Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRinthecellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection, 3LLcellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDHmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=3).Figure2.ProteinexpressionofGAPDHin3LLcells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedinto3LLcellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]Diabetologia.201255(7):2069-79.Thep47phox-andNADPHoxidaseorganiser1…Younetal [PDF]Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]JounalofBiologicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]RequestaQuotefor3LLTransfectionReagentAltogenBiosystems:AltogenBiosystemsmanufacturers celltypespecificandpreoptimizedtransfectionproducts, elecroporationkits,andinvivodeliveryreagentsforlifescienceresearch.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.AltogenResearchServices:AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).VolumeOptions:0.5ml(Catalog#1701)1.5ml(Catalog#1702)1.5mlCRISPR(Catalog#2206)8.0ml(Catalog#7015)
Altogen Biosystems全国代理部分产品列表：
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货号
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产品
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规格
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价格（美元）
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5030
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1703
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A204 Transfection Reagent (Rhabdomyosarcoma)
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0.5ml
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170
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1704
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A204 Transfection Reagent (Rhabdomyosarcoma)
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1.5ml
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348
窗体顶端
窗体底端
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2101
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A204 Transfection Reagent (Rhabdomyosarcoma)
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1.5ml CRISPR
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548
窗体顶端
窗体底端
|
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7018
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A204 Transfection Reagent (Rhabdomyosarcoma)
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8.0ml
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1044
窗体顶端
窗体底端
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1167
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A375 Cells Transfection Reagent (CRL-1619)
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0.5ml
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195
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1168
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A375 Cells Transfection Reagent (CRL-1620)
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1.5ml
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427
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2102
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A375 Cells Transfection Reagent (CRL-1621)
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1.5ml CRISPR
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627
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1169
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A375 Cells Transfection Reagent (CRL-1622)
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8.0ml
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1281
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5030
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Nanoparticle In Vivo Transfection Reagent
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0.5 ml - 10 injections
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445
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5010
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LIPID In Vivo Transfection Reagent
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0.5 ml - 10 injections
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405
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5040
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PEG-Liposome In Vivo Transfection Reagent
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0.5 ml - 10 injections
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465
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5020
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POLYMER In Vivo Transfection Reagent
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0.5 ml - 10 injections
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425
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5050
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Pancreas In Vivo Transfection Kit
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0.5 ml - 10 injections
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495
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5060
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Liver In Vivo Transfection Kit
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0.5 ml - 10 injections
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495
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5070
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Kidney In Vivo Transfection Kit
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0.5 ml - 10 injections
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495
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1721
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BMS2 Transfection Reagent (Bone Marrow Cells)
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0.5 ml
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231
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6345
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C6 Transfection Reagent (Glioma Cells, CCL-107)
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0.5 ml
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199
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6803
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DLD-1 Transfection Reagent (Colon Cancer, CCL-221)
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0.5 ml
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201
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1775
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HUH-7 Transfection Reagent (Liver Cancer Cells)
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0.5 ml
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179
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