DescriptionProductAvailABIlity:InStock(deliverywithin2-3businessdays,noextrachargefordrop-shipping).Ordering:ToplaceanorderviacreditcardorPOuseAddtoCartbutton(creatinganaccountisnotrequired).TransfectionReagentfor 3T3L1 Cells(EmbryonicFibroblast Cells,CL-173)ProprietarycationiclipidsformulationEffectiveandrobustintracellulardeliveryKitincludesTransfectionEnhancerreagentProducesconsistentresults,lot-to-lot,plate-to-plate,andwell-to-wellAprovenreagentforestablishingstablecelllinesOptimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethodsDownloadinvitro3T3-L1transfectionprotocol:[PDF]Download 3T3-L1CRISPR/Cas9transfectionprotocol:[PDF]DownloadPowerPointpresentationfor3T3-L1cellstransfectionkit:[PPT]DevelopedandmanufacturedbyAltogenBiosystemsTransfectionEfficiency:Reagentexhibitsatleast80%transfectionefficiencyofsiRNAdelivery.Transfectionefficiencywasdeterminedbyreal-timeRT-PCR.TransfectionProtocolandMSDS:DownloadAltogenBiosystems3T3 TransfectionProtocol:[PDF]DownloadMSDS:[PDF]3T3CellLine:The3T3-L1celllinewasestablishedin1974fromaSwissalbinomouse(Musmusculus)embryo.Thiscelllineexhibitsfibroblastmorphologyandisverysimilartothe3T3cellline,fromwhichitisderived.Thesecellsdonotcarryectromeliavirus(mousepox).3T3-L1couldbeusefulforstudyingfibroblastcells,ormammalianembryonictissue.Thiscelllinehasbeenknowntodoublenearlyevery14hours.3T3-L1cellsaresensitivetolipolyticandlipogenichormonesanddrugssuchasepinephrine,isoproterenol,andinsulin.Progressingfromarapidlydividingtoaconfluentandcontactinhibitedstate,3T3-L1celllineundergoesapre-ADIposetoadipose-likeconversion.3T3-L1isanembryonicmousefibroblastcelllinewidelyusedinthestudyofadiposetissueasitscellscandifferentiateintoadipocytesunderproperconditions.AltogenBiosystemsprovideslipid-basedtransfectionkitsforthe3T3-L1cellline.Data:Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedinto3T3L1cellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).Figure2.ProteinexpressionofLaminA/Cin3T3-L1cells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/Cweretransfectedinto3T3-L1cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]Diabetologia.201255(7):2069-79.Thep47phox-andNADPHoxidaseorganiser1…Younetal [PDF]BritishJournalofCancer.2012 107(3):516-26.TIGARinducesp53-mediatedcell-cycle…Madanetal[PDF]Hypertension.201463(2):353-61.Tissuetransglutaminasecontributesto…Liuetal[PDF]Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]JounalofBIOLOGicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]RequestaQuotefor3T3-L1TransfectionReagentAltogenBiosystems:AltogenBiosystemsisalifesciencescompany thatoffersover100celltype-specificandpre-optimizedtransfectionproducts, elecroporationkits,andinvivodeliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.AltogenResearchServices:AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).VolumeOptions:0.5ml(Catalog#1161)1.5ml(Catalog#1162)1.5mlCRISPR(Catalog#2207)8.0ml(Catalog#7016)
Altogen Biosystems全国代理部分产品列表：
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货号
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产品
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规格
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价格（美元）
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5030
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1703
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A204 Transfection Reagent (Rhabdomyosarcoma)
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0.5ml
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170
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1704
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A204 Transfection Reagent (Rhabdomyosarcoma)
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1.5ml
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348
窗体顶端
窗体底端
|
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2101
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A204 Transfection Reagent (Rhabdomyosarcoma)
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1.5ml CRISPR
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548
窗体顶端
窗体底端
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|
7018
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A204 Transfection Reagent (Rhabdomyosarcoma)
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8.0ml
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1044
窗体顶端
窗体底端
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1167
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A375 Cells Transfection Reagent (CRL-1619)
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0.5ml
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195
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1168
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A375 Cells Transfection Reagent (CRL-1620)
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1.5ml
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427
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2102
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A375 Cells Transfection Reagent (CRL-1621)
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1.5ml CRISPR
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627
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1169
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A375 Cells Transfection Reagent (CRL-1622)
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8.0ml
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1281
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5030
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Nanoparticle In Vivo Transfection Reagent
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0.5 ml - 10 injections
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445
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5010
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LIPID In Vivo Transfection Reagent
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0.5 ml - 10 injections
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405
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5040
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PEG-Liposome In Vivo Transfection Reagent
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0.5 ml - 10 injections
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465
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5020
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POLYMER In Vivo Transfection Reagent
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0.5 ml - 10 injections
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425
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5050
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Pancreas In Vivo Transfection Kit
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0.5 ml - 10 injections
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495
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5060
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Liver In Vivo Transfection Kit
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0.5 ml - 10 injections
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495
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5070
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Kidney In Vivo Transfection Kit
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0.5 ml - 10 injections
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495
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1721
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BMS2 Transfection Reagent (Bone Marrow Cells)
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0.5 ml
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231
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6345
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C6 Transfection Reagent (Glioma Cells, CCL-107)
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0.5 ml
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199
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6803
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DLD-1 Transfection Reagent (Colon Cancer, CCL-221)
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0.5 ml
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201
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1775
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HUH-7 Transfection Reagent (Liver Cancer Cells)
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0.5 ml
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179
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