DescriptionProductAvailABIlity:InStock(deliverywithin2-3businessdays,noextrachargefordrop-shipping).Ordering:ToplaceanorderviacreditcardorPOuseAddtoCartbutton(creatinganaccountisnotrequired).TransfectionReagentfor C6Cells(GliomaCells,CCL-107)TwocomponentformulationenhanceslipidmediatedtransfectionefficiencyOptimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNAKitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocolHightransfectionefficacyinthe presenceofserumExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmidReproducIBLetransfectionresultsWorkswellforstandardreversetransfectionandhigh-throughputapplicationsDownloadinvitroC6 transfectionprotocol:[PDF]Download C6CRISPR/Cas9transfectionprotocol:[PDF]DownloadPowerPointpresentationforC6cellstransfectionkit:[PPT]DevelopedandmanufacturedbyAltogenBiosystemsTransfectionEfficiency:Reagentexhibitsatleast94%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.TransfectionProtocolandMSDS:DownloadAltogenBiosystemsC6TransfectionProtocol:[PDF]DownloadMSDS:[PDF]C6 CellLine:Whileglioblastomaisnotthemostcommontypeofbraincancer,itisthemostaggressiveanddeadliest.Glialcellsareresponsibleforprovidingnourishmentandmechanicalsupporttonervecellsinthebrain.Gliomasaretumorsthatoriginatedinglialcellsandarecharacterizedbasedonlocation.AccordingtotheBrainTumorCenteratJohnsHopkins,malignantgliomascompriseabout70%ofallnewcasesofbraintumorsandtendtorecurdespiteaggressivecombinatorialtreatment.Newtherapiesaredesperatelyneededtoconqueranypotentialchallengesinthetreatmentofthemalignantbraintumorandimprovethesurvivalrateofpatients.Researchatthecellularlevelisahelpfultooltogatherinformationoncellsthatrapidlymetastasizesuchascancerousglialcells.TheC6gliomacelllinewasderivedfromthenervoustissueofarat(Rattusnorvegicus)glialtumorandhasproventobeareliabletransfectionhostforthestudyofbraintumors.ThisstrainofcellsisisolatedbyN-nitrosomethylureabyBendaetal.,exhibitingfibroblastmorphology.Inadditiontobeinganappropriatehostinmammalianbraintumorsstudies,theC6celllineisemployedforpreclinicalresearchworldwideaswellasincellularandmolecularBIOLOGyapplications.C6cellshavebeenknowntoshowglucocorticoidreceptors,andtheyalsomakeforagoodtransfectionresearchmodel.AltogenBiosystemsprovidestwo-componenthigh-efficiencytransfectionreagentkitsfortheC6gliomacellline.Data:Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoC6cellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).Figure2.ProteinexpressionofLaminA/CinC6cells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoC6cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.RequestaQuoteforC6TransfectionReagentAltogenBiosystems:AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifesciencesandcancerresearch.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykits(animaltransfection):1)Tissue-targetedreagents(deliveryofproteins,DNA,andRNAintoliver,pancreas,orkidneytissues),and2)Broadrangeinvivobiodistributionreagents(PEG-Liposomebasedreagent,Nanoparticle-basedinvivoreagent,Lipid-basedtransfectionkit,andPolymer-basedkit).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedbiomoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.AltogenLabsResearchServices:AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).VolumeOptions:0.5ml(Catalog#6345)1.5ml(Catalog#6346)1.5mlCRISPR(Catalog#2121)8.0ml(Catalog#7026)
Altogen Biosystems全国代理部分产品列表:
货号
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产品
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规格
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价格(美元)
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5030
|
|
|
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1703
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A204 Transfection Reagent (Rhabdomyosarcoma)
|
0.5ml
|
170
|
1704
|
A204 Transfection Reagent (Rhabdomyosarcoma)
|
1.5ml
|
348
窗体顶端
窗体底端
|
2101
|
A204 Transfection Reagent (Rhabdomyosarcoma)
|
1.5ml CRISPR
|
548
窗体顶端
窗体底端
|
7018
|
A204 Transfection Reagent (Rhabdomyosarcoma)
|
8.0ml
|
1044
窗体顶端
窗体底端
|
1167
|
A375 Cells Transfection Reagent (CRL-1619)
|
0.5ml
|
195
|
1168
|
A375 Cells Transfection Reagent (CRL-1620)
|
1.5ml
|
427
|
2102
|
A375 Cells Transfection Reagent (CRL-1621)
|
1.5ml CRISPR
|
627
|
1169
|
A375 Cells Transfection Reagent (CRL-1622)
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8.0ml
|
1281
|
5030
|
Nanoparticle In Vivo Transfection Reagent
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0.5 ml - 10 injections
|
445
|
5010
|
LIPID In Vivo Transfection Reagent
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0.5 ml - 10 injections
|
405
|
5040
|
PEG-Liposome In Vivo Transfection Reagent
|
0.5 ml - 10 injections
|
465
|
5020
|
POLYMER In Vivo Transfection Reagent
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0.5 ml - 10 injections
|
425
|
5050
|
Pancreas In Vivo Transfection Kit
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0.5 ml - 10 injections
|
495
|
5060
|
Liver In Vivo Transfection Kit
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0.5 ml - 10 injections
|
495
|
5070
|
Kidney In Vivo Transfection Kit
|
0.5 ml - 10 injections
|
495
|
1721
|
BMS2 Transfection Reagent (Bone Marrow Cells)
|
0.5 ml
|
231
|
6345
|
C6 Transfection Reagent (Glioma Cells, CCL-107)
|
0.5 ml
|
199
|
6803
|
DLD-1 Transfection Reagent (Colon Cancer, CCL-221)
|
0.5 ml
|
201
|
1775
|
HUH-7 Transfection Reagent (Liver Cancer Cells)
|
0.5 ml
|
179
|