Antigen InformationGene Symbols:CCL1 | CCL11 | CCL15 | CCL2 | CCL24 | CCL3 | CCL4 | CCL5 | CSF1 | CSF2| CSF3 | CXCL13 | CXCL8 | CXCL9 | ICAM1 | IFNG | IL10 | IL11 | IL12A | IL12B | IL13 | IL15 | IL16 | IL17A | IL1A | IL1B | IL1RN | IL2 | IL4 | IL5 | IL6 | IL6R | IL7 | LTA | PDGFB | TIMP1 | TIMP2 | TNF | TNFRSF1A | TNFRSF1B View more HideAssay FormatNumber of Targets Detected:40Species Detected:HumanCompatible Sample Types:Cell Culture SupernatantsCell LysatesOther Body FluidsPlasmaSerumTissue LysatesDesign Principle:Sandwich-basedMethod of Detection:Fluorescence Laser ScannerQuantitative/Semi-Quantitative:QuantitativeSolid Support:Glass SlideProduct SpecificationsSize:1, 2, or 4 Slide Kit (16 sub-arrays per slide)Product FeaturesMore cost-effective than traditional ELISAHigh specificity and system reproducibilitySuitable for diverse sample typesLow sample volume requirement: 50 µL or lessGet results same day (6-hour processing time)Well-suited for high throughput assaysQ Analyzer software provides one-step computationTarget NamesScroll over each target protein for more informationBLC(CXCL13)Gene Symbol: CXCL13Gene ID: 10563Accession #: O43927ELISA Kit: RayBio Human BLC ELISAEotaxin-1(CCL11)Gene Symbol: CCL11Gene ID: 6356Accession #: P51671ELISA Kit: RayBio Human Eotaxin-1 ELISAEotaxin-2(MPIF-2/CCL24)Gene Symbol: Ccl24Gene ID: 6369Accession #: O00175ELISA Kit: RayBio Human Eotaxin-2 ELISAGCSFGene Symbol: CSF3Gene ID: 1440Accession #: P09919ELISA Kit: RayBio Human G-CSF ELISAGM-CSFGene Symbol: CSF2Gene ID: 1437Accession #: P04141ELISA Kit: RayBio Human GM-CSF ELISAI-309(TCA-3/CCL1)Gene Symbol: CCL1Gene ID: 6346Accession #: P22362ELISA Kit: RayBio Human I-309 ELISAICAM-1(CD54)Gene Symbol: ICAM1Gene ID: 3383Accession #: P05362ELISA Kit: RayBio Human sICAM-1 ELISAIFN-gammaGene Symbol: IFNGGene ID: 3458Accession #: P01579ELISA Kit: RayBio Human IFN-gamma ELISAIL-1 alpha(IL-1 F1)Gene Symbol: IL1AGene ID: 3552Accession #: P01583ELISA Kit: RayBio Human IL-1 alpha ELISAIL-1 beta(IL-1 F2)Gene Symbol: IL1BGene ID: 3553Accession #: P01584ELISA Kit: RayBio Human IL-1 beta ELISAIL-1 ra(IL-1 F3)Gene Symbol: IL1RNGene ID: 3557Accession #: P18510ELISA Kit: RayBio Human IL-1 RA ELISAIL-2Gene Symbol: IL2Gene ID: 3558Accession #: P60568ELISA Kit: RayBio Human IL-2 ELISAIL-4Gene Symbol: IL4Gene ID: 3565Accession #: P05112ELISA Kit: RayBio Human IL-4 ELISAIL-5Gene Symbol: IL5Gene ID: 3567Accession #: P05113ELISA Kit: RayBio Human IL-5 ELISAIL-6Gene Symbol: IL6Gene ID: 3569Accession #: P05231ELISA Kit: RayBio Human IL-6 ELISAIL-6 RGene Symbol: IL6RGene ID: 3570Accession #: P08887ELISA Kit: RayBio Human IL-6 sR ELISAIL-7Gene Symbol: IL7Gene ID: 3574Accession #: P13232ELISA Kit: RayBio Human IL-7 ELISAIL-8(CXCL8)Gene Symbol: IL8Gene ID: 3576Accession #: P10145ELISA Kit: RayBio Human IL-8 ELISAIL-10Gene Symbol: IL10Gene ID: 3586Accession #: P22301ELISA Kit: RayBio Human IL-10 ELISAIL-11Gene Symbol: IL11Gene ID: 3589Accession #: P20809ELISA Kit: RayBio Human IL-11 ELISAIL-12 p40Gene Symbol: IL12BGene ID: 3593Accession #: P29460ELISA Kit: RayBio Human IL-12 p40 ELISAIL-12 p70Gene Symbol: IL12AGene ID: 3592Accession #: P29459ELISA Kit: RayBio Human IL-12 p70 ELISAIL-13Gene Symbol: IL13Gene ID: 3596Accession #: P35225ELISA Kit: RayBio Human IL-13 ELISAIL-15Gene Symbol: IL15Gene ID: 3600Accession #: P40933ELISA Kit: RayBio Human IL-15 ELISAIL-16Gene Symbol: IL16Gene ID: 3603Accession #: Q14005ELISA Kit: RayBio Human IL-16 ELISAIL-17AGene Symbol: IL17AGene ID: 3605Accession #: Q16552MCP-1(CCL2)Gene Symbol: CCL2Gene ID: 6347Accession #: P13500ELISA Kit: RayBio Human MCP-1 ELISAM-CSFGene Symbol: CSF1Gene ID: 1435Accession #: P09603ELISA Kit: RayBio Human M-CSF ELISAMIG(CXCL9)Gene Symbol: CXCL9Gene ID: 4283Accession #: Q07325ELISA Kit: RayBio Human MIG ELISAMIP-1 alpha(CCL3)Gene Symbol: CCL3Gene ID: 6348Accession #: P10147ELISA Kit: RayBio Human MIP-1 alpha ELISAMIP-1 beta(CCL4)Gene Symbol: CCL4Gene ID: 6351Accession #: P13236ELISA Kit: RayBio Human MIP-1 beta ELISAMIP-1 delta(CCL15)Gene Symbol: CCL15Gene ID: 6359Accession #: Q16663ELISA Kit: RayBio Human MIP-1 delta ELISAPDGF-BBGene Symbol: PDGFBGene ID: 5155Accession #: P01127ELISA Kit: RayBio Human PDGF-BB ELISARANTES(CCL5)Gene Symbol: CCL5Gene ID: 6352Accession #: P13501ELISA Kit: RayBio Human RANTES ELISATIMP-1Gene Symbol: TIMP1Gene ID: 7076Accession #: P01033ELISA Kit: RayBio Human TIMP-1 ELISATIMP-2Gene Symbol: TIMP2Gene ID: 7077Accession #: P16035ELISA Kit: RayBio Human TIMP-2 ELISATNF alphaGene Symbol: TNFGene ID: 7124Accession #: P01375ELISA Kit: RayBio Human TNF-alpha ELISATNF beta(TNFSF1B)Gene Symbol: LTAGene ID: 4049Accession #: P01374ELISA Kit: RayBio Human TNF-beta ELISATNF RI(TNFRSF1A)Gene Symbol: TNFRSF1AGene ID: 7132Accession #: P19438ELISA Kit: RayBio Human sTNFRI ELISATNF RII(TNFRSF1B)Gene Symbol: TNFRSF1BGene ID: 7133Accession #: P20333ELISA Kit: RayBio Human sTNFRII ELISAApplication NotesSuggested ApplicationMultiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal RangeKit ComponentsHuman Inflammation Array Q3 Slide(s)Blocking BufferWash Buffer 1Wash Buffer 2Lyophilized Standard MixBiotinylated Detection Antibody CocktailStreptavidin-Conjugated FluorSlide Washer/DryerAdhesive Plastic StripsManualOther Materials RequiredDistilled or deionized waterSmall plastic boxes or containersPipettors, pipette tips and other common lab consumablesOrbital shaker or oscillating rockerAluminum foilGene microarray scanner or similar laser fluorescence scannerView Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn MoreProtocol OutlineDry the glass slidePrepare StandardsBlock array surfaceIncubate with Samples and StandardsIncubate with Biotinylated Detection Antibody CocktailIncubate with Streptavidin-Conjugated FluorDisassemble the glass slideScan with a gene microarray laser scannerPerform densitometry and analysisStorage/StabilityFor best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.Platonov M., Borovjagin AV., Kaverina N., et al. KISS1 tumor suppressor restricts angiogenesis of breast cancer brain metastases and sensitizes them to oncolytic virotherapy in vitro. Cancer Lett. 2018 Mar 28;417:75-88. doi: 10.1016/j.canlet.2017.12.024. Species: HumanSample type: Conditioned MediaDillman R., Cornforth A., Nistor G., et al. Randomized phase II trial of autologous dendritic cell vaccines versus autologous tumor cell vaccines in metastatic melanoma: 5-year follow up and additional analyses. J Immunother Cancer. 2018 Mar 6;6(1):19. doi: 10.1186/s40425-018-0330-1.Species: HumanSample type: SerumLiao Y., Ivanova L., Zhu H., et al. Cord Blood-Derived Stem Cells Suppress Fibrosis and May Prevent Malignant Progression in Recessive Dystrophic Epidermolysis Bullosa. Stem Cells. 2018 Sep 24. doi: 10.1002/stem.2907. Species: HumanSample type: Conditioned MediaLeung GP., Feng T., Sigoillot FD., et al. Hyperactivation of MAPK Signaling Is Deleterious to RAS/RAF-mutant Melanoma. Mol Cancer Res. 2018 Sep 10. doi: 10.1158/1541-7786.MCR-18-0327Species: HumanSample type: Conditioned MediaIzrael M., Slutsky SG., Admoni T., et al. Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1G93A and NSG animal models. Stem Cell Res Ther. 2018 Jun 6;9(1):152. doi: 10.1186/s13287-018-0890-5.Species: HumanSample type: Conditioned MediaReategui E., Fatemeh J., Khankhel A., et al. Microscale arrays for the profiling of start and stop signals coordinating human-neutrophil swarming. Nature Biomedical Engineering 1, Article number: 0094 (2017) doi:10.1038/s41551-017-0094Species: HumanSample type: Conditioned MediaWang S., Lu Y., Hong Q., et al. Protein Array-Based Detection of Proteins in Kidney Tissues from Patients with Membranous Nephropathy. Biomed Res Int. 2017; 2017: 7843584. doi: 10.1155/2017/7843584Species: HumanSample type: Tissue LysateVelez G., Bassuk A., Colgan D., et al. Therapeutic drug repositioning using personalized proteomics of liquid biopsies. JCI Insight. 2017 Dec 21;2(24). pii: 97818. doi: 10.1172/jci.insight.97818.Species: HumanSample type: Vitreous FluidYang B., Deng Q., Zhang W., et al. Exposure to Polycyclic Aromatic Hydrocarbons, Plasma Cytokines, and Heart Rate Variability. Sci Rep. 2016 Jan 13;6:19272. doi: 10.1038/srep19272.Species: HumanSample type: PlasmaGu R., Zhou M., Jiang C., et al. Elevated concentration of cytokines in aqueous in post-vitrectomy eyes. Clin Exp Ophthalmol. 2016 Mar;44(2):128-34. doi: 10.1111/ceo.12638Species: HumanSample type: Aqueous HumorPrev123NextMy lab members have used this array several times before, they recommend it to me, both mouse's and human's are very reliable!Xue HaoThe Wistar Institute11/21/2019, 07:38 PMIt's good and easy to use, but need a laser scanner.Xingmin SunUniversity of South Florida09/19/2018, 10:03 AMWrite A ReviewManualSDSGALAnalysis Tool