Assay FormatNumber of Targets Detected:48Species Detected:HumanCompatible Sample Types:SerumMethod of Detection:Fluorescence Laser ScannerQuantitative/Semi-Quantitative:Semi-QuantitativeSolid Support:Glass SlideProduct SpecificationsSize:16 Sample KitProduct FeaturesSimultaneous detection of human IgE and IgG associated with 48 allergen proteinsHigh sensitivityLarge dynamic rangeLow sample volume required (10 - 100 µl per array)Suitable for high-throughput assaysHigh efficiency and accuracyAffordable, quick and simple to useCustomizable: Tell us the allergens you"re interested inTest 16 samples on each slideTarget NamesNo.Allergen NameSpecies - Scientific Name1Act c 1Kiwi - Actinidia chinensis2Act c 2Kiwi - Actinidia chinensis3Alt a 1Alternaria rot fungus - Alternaria alternata4Ana c 2Pineapple - Ananas comosus5Ara h 1Peanut - Arachis hypogaea6Ara h 2Peanut - Arachis hypogaea7Ara h 3Peanut - Arachis hypogaea8Ara h 6Peanut - Arachis hypogaea9Art v 3Mugwort - Artemisia vulgaris10Asp f 1Aspergillus fumigatus11Bla g 5German cockroach - Blattella germanica12Bla g 6German cockroach - Blattella germanica13Bos d 6Cow (bovine) - Bos taurus14Can f 1Dog (canine) - Canis familiaris15Cas s 8Sweet Chestnut - Castanea sativa16Che a 2Lamb"s-quarters - Chenopodium album17Che a 3Lamb"s-quarters - Chenopodium album18Cla h 5Mycosphaerella tassiana - Davidiella tassiana (Cladosporium herbarum)19Cla h 8Mycosphaerella tassiana - Davidiella tassiana (Cladosporium herbarum)20Cor a 14European hazelnut - Corylus avellana21Cor a 9European hazelnut - Corylus avellana22Cte f 1Cat flea - Ctenocephalides felis23Cuc m 2Muskmelon - Cucumis melo24Der f 2American house dust mite - Dermatophagoides farinae25Der p 1European house dust mite - Dermatophagoides pteronyssinus26Der p 2European house dust mite - Dermatophagoides pteronyssinus27Fel d 1Cat (feline) - Felis catus28Fus c 1Fusarium culmorum29Gly d 2House itch mite - Glycyphagus domesticus30Jug r 1English walnut - Juglans regia31Jug r 2English walnut - Juglans regia32Jug r 3English walnut - Juglans regia33Jug r 4English walnut - Juglans regia34Lep d 2Fodder mite - Lepidoglyphus destructor35Mal d 2Apple - Malus domestica36Mal d 3Apple - Malus domestica37Mus a 4Banana - Musa acuminata38Mus m 1Mouse - Mus musculus39Pen c 3Penicillium citrinum40Pen n 13Penicillium notatum - Penicillium chrysogenum41Per a 1American cockroach - Periplaneta americana42Phl p 1Common timothy - Phleum pratense43Phl p 12Common timothy - Phleum pratense44Pho d 2Date palm - Phoenix dactylifera45Pru p 2.2Prunus dulcis x Prunus persica46Pru p 3Peach - Prunus persica47Rat n 1Rat - Rattus norvegicus48Tri a 14Wheat - Triticum aestivumApplication NotesSuggested ApplicationCommon allergen detectionKit ComponentsRayBio® Human Allergen Protein Array Glass SlideRayBio® G series antibody array accessory (including slide incubation chamber assembly, Gasket, Protective cover, Snap-on sides and adhesive film)Blocking BufferWash Buffer 1Wash Buffer 2Biotin-conjugated anti-human IgEBiotin-conjugated anti-human IgGCy3-Conjugated StreptavidinOther Materials RequiredOrbital shaker or oscillating rockerPipettors, pipette tips and other common lab consumablesSmall plastic boxes or containersDistilled waterAluminum foilLaser scanner for fluorescence detection (compatible laser scanners) If you do not have access to a compatible laser scanner RayBiotech can provide free scanning service.Protocol OutlineSample incubation (1-2 hours)Biotin-conjugated antibody incubation (1-2 hours)Cy3-Streptavidin incubation (1 hour)Scan and data analysis Use the Analysis Tool for Human Allergen Protein Array G1 to quickly perform data analysis (background subtraction, normalization, etc.).Storage/StabilityUpon receipt, all components of the RayBio® Human Allergen Protein Array kit should be stored at -20 °C to -80 °C until just before the experiment. At -20 °C to -80 °C the kit will retain complete activity for up to 6 months. Please use within six months of purchase. Once thawed, the array glass chip and Blocking Buffer should be kept at –20 °C and all other component should be stored at 4 °C Use within three months after reagents have been thawed.No posts foundWrite A ReviewManual SDSAnalysis Tool