Antigen InformationGene Symbols:CCL2 | CCL5 | CSF1 | CSF2 | CXCL1 | IFNG | IL10 | IL12A | IL13 | IL17A| IL1A | IL1B | IL2 | IL3 | IL4 | IL5 | IL6 | IL9 | TNF | VEGFA View more HideAssay FormatPathway:MAPK SignalingNumber of Targets Detected:20Species Detected:MouseCompatible Sample Types:Cell Culture SupernatantsCell LysatesOther Body FluidsPlasmaSerumTissue LysatesDesign Principle:Sandwich-basedMethod of Detection:Fluorescence Laser ScannerQuantitative/Semi-Quantitative:QuantitativeSolid Support:Glass SlideProduct SpecificationsSize:1, 2, or 4 Slide Kit (16 sub-arrays per slide)Product FeaturesMore cost-effective than traditional ELISAHigh specificity and system reproducibilitySuitable for diverse sample typesLow sample volume requirement: 50 µL or lessGet results same day (6-hour processing time)Well-suited for high throughput assaysQ Analyzer software provides one-step computationTarget NamesScroll over each target protein for more informationGM-CSFGene Symbol: CSF2Accession #: P26955ELISA Kit: RayBio Mouse GM-CSF ELISAIFN-gammaGene Symbol: IFNGAccession #: P01580ELISA Kit: RayBio Mouse IFN-gamma ELISAIL-1 alpha(IL-1 F1)Gene Symbol: IL1AAccession #: P01582ELISA Kit: RayBio Mouse IL-1 alpha ELISAIL-1 beta(IL-1 F2)Gene Symbol: IL1BAccession #: P10749ELISA Kit: RayBio Mouse IL-1 beta ELISAIL-10Gene Symbol: IL10Accession #: Q0VBJ1ELISA Kit: RayBio Mouse IL-10 ELISAIL-12 p70Gene Symbol: IL12AAccession #: P43431IL-13Gene Symbol: IL13Accession #: P20109ELISA Kit: RayBio Mouse IL-13 ELISAIL-17AGene Symbol: IL17AAccession #: Q60971ELISA Kit: RayBio Mouse IL-17A ELISAIL-2Gene Symbol: IL2Accession #: Q791T3ELISA Kit: RayBio Mouse IL-2 ELISAIL-3Gene Symbol: IL3Accession #: P01586ELISA Kit: RayBio Mouse IL-3 ELISAIL-4Gene Symbol: IL4Accession #: P07750ELISA Kit: RayBio Mouse IL-4 ELISAIL-5Gene Symbol: IL5Accession #: P04401ELISA Kit: RayBio Mouse IL-5 ELISAIL-6Gene Symbol: IL6Accession #: P08505ELISA Kit: RayBio Mouse IL-6 ELISAIL-9Gene Symbol: IL9Accession #: P15247ELISA Kit: RayBio Mouse IL-9 ELISAKC(CXCL1)Gene Symbol: CXCL1Accession #: P12850ELISA Kit: RayBio Mouse KC ELISAMCP-1(CCL2)Gene Symbol: CCL2Accession #: Q9QYD7ELISA Kit: RayBio Mouse MCP-1 ELISAM-CSFGene Symbol: CSF1Accession #: P07141ELISA Kit: RayBio Mouse M-CSF ELISARANTES(CCL5)Gene Symbol: CCL5Accession #: P30882ELISA Kit: RayBio Mouse RANTES ELISATNF alphaGene Symbol: TNFAccession #: Q62326ELISA Kit: RayBio Mouse TNF-alpha ELISAVEGF-AGene Symbol: VEGFAAccession #: Q00731ELISA Kit: RayBio Mouse VEGF-A ELISAApplication NotesSuggested ApplicationMultiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal RangeKit ComponentsMouse Cytokine Array Q1 Slide(s)Blocking BufferWash Buffer 1Wash Buffer 2Lyophilized Standard MixBiotinylated Detection Antibody CocktailStreptavidin-Conjugated FluorSlide Washer/DryerAdhesive Plastic StripsManualOther Materials RequiredDistilled or deionized waterSmall plastic boxes or containersPipettors, pipette tips and other common lab consumablesOrbital shaker or oscillating rockerAluminum foilGene microarray scanner or similar laser fluorescence scannerView Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn MoreProtocol OutlineDry the glass slidePrepare StandardsBlock array surfaceIncubate with Samples and StandardsIncubate with Biotinylated Detection Antibody CocktailIncubate with Streptavidin-Conjugated FluorDisassemble the glass slideScan with a gene microarray laser scannerPerform densitometry and analysisStorage/StabilityFor best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.Fink DM, Connor AL, Kelley PM, Steele MM, Hollingsworth MA, Tempero RM. Nerve Growth Factor Regulates Neurolymphatic Remodeling during Corneal Inflammation and Resolution. Ng LG, ed. PLoS ONE 2014;9(11):e112737. doi:10.1371/journal.pone.0112737.Species: MouseSample type: Tissue LysateHuang X, Ren L, Ye P, Cheng C, Wu J, et al. (2014) Peroxisome Proliferator Activated Receptor c Deficiency in T Cells Accelerates Chronic Rejection by Influencing the Differentiation of CD4+ T Cells and Alternatively Activated Macrophages. PLoS ONE 9(11): e112953. doi:10.1371/journal.pone.0112953Species: MouseSample type: Conditioned MediaKarra L., Haworth O., Priluck R., et al. Lipoxin B4 promotes the resolution of allergic inflammation in the upper and lower airways of mice. Mucosal Immunol. 2014 Dec 3. doi: 10.1038/mi.2014.116.Species: MouseSample type: SerumGuo H., Yun C., Hou G., Du J., Huang X., et al. Mangiferin Attenuates Th1/Th2 Cytokine Imbalance in an Ovalbumin-Induced Asthmatic Mouse Model. PLOS One. Published: June 23, 2014. DOI: 10.1371/journal.pone.0100394Species: MouseSample type: SerumZingariello, Maria, et al. "Characterization of the TGF-?1 signaling abnormalities in the Gata1 low mouse model of myelofibrosis."?Blood?121.17 (2013): 3345-3363.Species: MouseSample type: Conditioned MediaArevalo-Martin A., Molina-Holgado E., Guaza C. A CB?/CB? receptor agonist, WIN 55,212-2, exerts its therapeutic effect in a viral autoimmune model of multiple sclerosis by restoring self-tolerance to myelin. Neuropharmacology. 2012 Sep;63(3):385-93. doi: 10.1016/j.neuropharm.2012.04.012. Species: MouseSample type: Tissue LysateRobbins D., Li W., Humphrey K., Zhao Y. Redox Factor-1 Mediates Inflammatory Response during Tumor Promotion in Skin Epidermal JB6 Cells. Open Journal of Apoptosis, 2012, 1, 19-24. http://dx.doi.org/10.4236/ojapo.2012.13003Species: MouseSample type: Cell LysateVila` L, Roglans N, Baena M, Barroso E, Alegret M, et al. (2012) Metabolic Alterations and Increased Liver mTOR Expression Precede the Development of Autoimmune Disease in a Murine Model of Lupus Erythematosus. PLoS ONE 7(12): e51118. doi:10.1371/journal.pone.0051118Species: MouseSample type: Conditioned MediaKelley P., et al. Regressed lymphatic vessels develop during corneal repair. Lab Invest. 2011 Nov;91(11):1643-51. doi: 10.1038/labinvest.2011.121.Species: MouseSample type: Tissue LysateOjeda, Gloria, et al. "Complement regulatory protein Crry/p65 costimulation expands natural Treg cells with enhanced suppressive properties in proteoglycan?induced arthritis."?Arthritis & Rheumatism?63.6 (2011): 1562-1572.Species: MouseSample type: Conditioned MediaPrev12NextVery easy! I recommend their scanning service!!!Ondrej UherNIH08/30/2019, 04:07 PMI used quite a a lot of cytokine and chemokine array kit from Raybiotech.These product is very simple and reliable to measure the production of cytokine/chemokine from cell culture media.They also provide a free Scanning service, so no need to worry about slide scanner. Highly recommended.Sang LeeNational Institute of health09/04/2018, 01:28 PMWrite A ReviewManualSDS GALAnalysis Tool