Antigen InformationGene Symbols:AGRP | ANG | ANGPT1 | ANGPT2 | ANGPTL4 | CCL1 | CCL13 | CCL2 | CCL5 | CCL7| CCL8 | CSF2 | CSF3 | CXCL1 | CXCL10 | CXCL11 | CXCL16 | CXCL2 | CXCL3 | CXCL5 | CXCL8 | EGF | FGF2 | FGF4 | FIGF | FLT4 | FST | HBEGF | HGF | IFNG | IGF1 | IL10 | IL12A | IL12B | IL17A | IL1A | IL1B | IL2 | IL4 | IL6 | INHBA | KDR | LEP | LIF | LTA | MMP1 | MMP9 | PDGFB | PECAM1 | PGF | PLAUR | PLG | TEK | TGFA | TGFB1 | TGFB3 | THPO | TIE1 | TIMP1 | TIMP2 | TNF | TPO | VEGFA View more HideAssay FormatNumber of Targets Detected:60Species Detected:HumanCompatible Sample Types:Cell Culture SupernatantsCell LysatesOther Body FluidsPlasmaSerumTissue LysatesDesign Principle:Sandwich-basedMethod of Detection:Fluorescence Laser ScannerQuantitative/Semi-Quantitative:QuantitativeSolid Support:Glass SlideProduct SpecificationsSize:1, 2, or 4 Slide Kit (16 sub-arrays per slide)Product FeaturesMore cost-effective than traditional ELISAHigh specificity and system reproducibilitySuitable for diverse sample typesLow sample volume requirement: 100 µL or lessGet results same day (6-hour processing time)Well-suited for high throughput assaysQ Analyzer software provides one-step computationTarget NamesScroll over each target protein for more informationActivin AGene Symbol: INHBAGene ID: 3624Accession #: P08476ELISA Kit: RayBio Human Activin A ELISAAgRPGene Symbol: AgRPGene ID: 181Accession #: O00253ELISA Kit: RayBio Human AgRP ELISAAngiopoietin-1Gene Symbol: ANGPT1Gene ID: 284Accession #: Q15389ELISA Kit: RayBio Human ANGPT1 ELISAAngiopoietin-2Gene Symbol: ANGPT2Gene ID: 285Accession #: O15123ELISA Kit: RayBio Human ANGPT2 ELISAAngiogeninGene Symbol: ANGGene ID: 283Accession #: P03950ELISA Kit: RayBio Human Angiogenin ELISAAngiostatinGene Symbol: PLGGene ID: 5340Accession #: P00747ELISA Kit: RayBio Human Angiostatin ELISAANGPTL4Gene Symbol: ANGPTL4Gene ID: 51129Accession #: Q9BY76ELISA Kit: RayBio Human ANGPTL4 ELISAbFGFGene Symbol: FGF2Gene ID: 2247Accession #: P09038ELISA Kit: RayBio Human bFGF ELISACXCL16Gene Symbol: CXCL16Gene ID: 58191Accession #: Q9H2A7ELISA Kit: RayBio Human CXCL16 ELISAEGFGene Symbol: EGFGene ID: 1950Accession #: P01133ELISA Kit: RayBio Human EGF ELISAENA-78(CXCL5)Gene Symbol: CXCL5Gene ID: 6374Accession #: P42830ELISA Kit: RayBio Human ENA-78 ELISAFGF-4Gene Symbol: FGF4Gene ID: 2249Accession #: P08620ELISA Kit: RayBio Human FGF-4 ELISAFollistatinGene Symbol: FSTGene ID: 10468Accession #: P19883ELISA Kit: RayBio Human Follistatin ELISAGCSFGene Symbol: CSF3Gene ID: 1440Accession #: P09919ELISA Kit: RayBio Human G-CSF ELISAGM-CSFGene Symbol: CSF2Gene ID: 1437Accession #: P04141ELISA Kit: RayBio Human GM-CSF ELISAGRO alpha/beta/gammaGene Symbol: CXCL1/CXCL2/CXCL3Gene ID: 2919/2920/2921Accession #: P09341/P19875/P19876HB-EGFGene Symbol: HBEGFGene ID: 1839Accession #: Q99075ELISA Kit: RayBio Human HB-EGF ELISAHGFGene Symbol: HGFGene ID: 3082Accession #: P14210ELISA Kit: RayBio Human HGF ELISAI-309(TCA-3/CCL1)Gene Symbol: CCL1Gene ID: 6346Accession #: P22362ELISA Kit: RayBio Human I-309 ELISAIFN-gammaGene Symbol: IFNGGene ID: 3458Accession #: P01579ELISA Kit: RayBio Human IFN-gamma ELISAIGF-1Gene Symbol: IGF1Gene ID: 3479Accession #: P05019ELISA Kit: RayBio Human IGF-1 ELISAIL-10Gene Symbol: IL10Gene ID: 3586Accession #: P22301ELISA Kit: RayBio Human IL-10 ELISAIL-12 p40Gene Symbol: IL12BGene ID: 3593Accession #: P29460ELISA Kit: RayBio Human IL-12 p40 ELISAIL-12 p70Gene Symbol: IL12AGene ID: 3592Accession #: P29459ELISA Kit: RayBio Human IL-12 p70 ELISAIL-17AGene Symbol: IL17AGene ID: 3605Accession #: Q16552ELISA Kit: RayBio Human IL-17A ELISAIL-1 alpha(IL-1 F1)Gene Symbol: IL1AGene ID: 3552Accession #: P01583ELISA Kit: RayBio Human IL-1 alpha ELISAIL-1 beta(IL-1 F2)Gene Symbol: IL1BGene ID: 3553Accession #: P01584ELISA Kit: RayBio Human IL-1 beta ELISAIL-2Gene Symbol: IL2Gene ID: 3558Accession #: P60568ELISA Kit: RayBio Human IL-2 ELISAIL-4Gene Symbol: IL4Gene ID: 3565Accession #: P05112ELISA Kit: RayBio Human IL-4 ELISAIL-6Gene Symbol: IL6Gene ID: 3569Accession #: P05231ELISA Kit: RayBio Human IL-6 ELISAIL-8(CXCL8)Gene Symbol: IL8Gene ID: 3576Accession #: P10145ELISA Kit: RayBio Human IL-8 ELISAIP-10(CXCL10)Gene Symbol: CXCL10Gene ID: 3627Accession #: P02778ELISA Kit: RayBio Human IP-10 ELISAI-TAC(CXCL11)Gene Symbol: CXCL11Gene ID: 6373Accession #: O14625ELISA Kit: RayBio Human I-TAC ELISALeptinGene Symbol: LEPGene ID: 3952Accession #: P41159ELISA Kit: RayBio Human Leptin ELISALIFGene Symbol: LIFGene ID: 3976Accession #: P15018ELISA Kit: RayBio Human LIF ELISAMCP-1(CCL2)Gene Symbol: CCL2Gene ID: 6347Accession #: P13500ELISA Kit: RayBio Human MCP-1 ELISAMCP-2(CCL8)Gene Symbol: CCL8Gene ID: 6355Accession #: P80075ELISA Kit: RayBio Human MCP-2 ELISAMCP-3(MARC/CCL7)Gene Symbol: CCL7Gene ID: 6354Accession #: P80098ELISA Kit: RayBio Human MCP-3 ELISAMCP-4(CCL13)Gene Symbol: CCL13Gene ID: 6357Accession #: Q99616ELISA Kit: RayBio Human MCP-4 ELISAMMP-1Gene Symbol: MMP1Gene ID: 4312Accession #: P03956ELISA Kit: RayBio Human MMP-1 ELISAMMP-9Gene Symbol: MMP9Gene ID: 4318Accession #: P14780ELISA Kit: RayBio Human MMP-9 ELISAPDGF-BBGene Symbol: PDGFBGene ID: 5155Accession #: P01127ELISA Kit: RayBio Human PDGF-BB ELISAPECAM-1(CD31)Gene Symbol: PECAM1Gene ID: 5175Accession #: P16284ELISA Kit: RayBio Human PECAM-1 ELISAPLGFGene Symbol: PGFGene ID: 5228Accession #: P49763ELISA Kit: RayBio Human PLGF ELISARANTES(CCL5)Gene Symbol: CCL5Gene ID: 6352Accession #: P13501ELISA Kit: RayBio Human RANTES ELISATGF alphaGene Symbol: TGFAGene ID: 7039Accession #: P01135ELISA Kit: RayBio Human TGF-alpha ELISATGF beta 1Gene Symbol: TGFB1Gene ID: 7040Accession #: P01137ELISA Kit: RayBio Human TGF-beta 1 ELISATGF beta 3Gene Symbol: TGFB3Gene ID: 7043Accession #: P10600Tie-1Gene Symbol: TIE1Gene ID: 7075Accession #: P35590ELISA Kit: RayBio Human Tie-1 ELISATie-2Gene Symbol: TEKGene ID: 7010Accession #: Q02763ELISA Kit: RayBio Human Tie-2 ELISATIMP-1Gene Symbol: TIMP1Gene ID: 7076Accession #: P01033ELISA Kit: RayBio Human TIMP-1 ELISATIMP-2Gene Symbol: TIMP2Gene ID: 7077Accession #: P16035ELISA Kit: RayBio Human TIMP-2 ELISATNF alphaGene Symbol: TNFGene ID: 7124Accession #: P01375ELISA Kit: RayBio Human TNF-alpha ELISATNF beta(TNFSF1B)Gene Symbol: LTAGene ID: 4049Accession #: P01374ELISA Kit: RayBio Human TNF-beta ELISAThrombopoietin(TPO)Gene Symbol: THPOGene ID: 7066Accession #: P40225ELISA Kit: RayBio Human TPO ELISAuPARGene Symbol: PLAURGene ID: 5329Accession #: Q03405ELISA Kit: RayBio Human uPAR ELISAVEGF-AGene Symbol: VEGFAGene ID: 7422Accession #: P15692ELISA Kit: RayBio Human VEGF-A ELISAVEGFR2Gene Symbol: KDRGene ID: 3791Accession #: P35968ELISA Kit: RayBio Human VEGF R2 ELISAVEGFR3Gene Symbol: FLT4Gene ID: 2324Accession #: P35916ELISA Kit: RayBio Human VEGF R3 ELISAVEGF-DGene Symbol: FIGFGene ID: 2277Accession #: O43915ELISA Kit: RayBio Human VEGF-D ELISASpecies DetectedHumanApplication NotesSuggested ApplicationMultiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal RangeKit ComponentsHuman Angiogenesis Array Q1000 Slide(s)Blocking BufferWash Buffer 1Wash Buffer 2Lyophilized Standard MixBiotinylated Detection Antibody CocktailStreptavidin-Conjugated FluorSlide Washer/DryerAdhesive Plastic StripsManualOther Materials RequiredDistilled or deionized waterSmall plastic boxes or containersPipettors, pipette tips and other common lab consumablesOrbital shaker or oscillating rockerAluminum foilGene microarray scanner or similar laser fluorescence scannerView Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn MoreProtocol OutlineDry the glass slidePrepare StandardsBlock array surfaceIncubate with Samples and StandardsIncubate with Biotinylated Detection Antibody CocktailIncubate with Streptavidin-Conjugated FluorDisassemble the glass slideScan with a gene microarray laser scannerPerform densitometry and analysisStorage/StabilityFor best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.Schuster C., Akslen L., Stokowy T., Straume O. Predictive value of angiogenic proteins in patients with metastatic melanoma treated with bevacizumab monotherapy. J Pathol Clin Res. 2019 Jan;5(1):53-62. doi: 10.1002/cjp2.116. Species: HumanSample type: SerumZbucka-Kretowska, Monika, et al. "Amniotic fluid angiogenic and inflammatory factor profiling in foetal down syndrome."?Fetal diagnosis and therapy?44 (2018): 44-50.Species: HumanSample type: Amniotic fluidChen J, Liu Z, Hong MM, Zhang H, Chen C, et al. (2014) Proangiogenic Compositions of Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells. PLoS ONE 9(12): e115316. doi:10.1371/journal.pone.0115316Species: HumanSample type: Cell LysateKoob T., Lim J., Massee M., Zabek N., Rennet R., Gurtner G., Li W. Angiogenic properties of dehydrated human amnion/chorion allografts. Vascular Cell 2014,6:10 doi:10.1186/2045-824X-6-10 [Epub Ahead of Print]therapeutic potential for soft tissue repair and regenerationSpecies: HumanSample type: Conditioned MediaStorti, P., et al. "HOXB7 expression by myeloma cells regulates their pro-angiogenic properties in multiple myeloma patients."?Leukemia?25.3 (2011): 527-537.Species: HumanSample type: Cell LysateGujar, Shashi A., et al. "Oncolytic virus-initiated protective immunity against prostate cancer."?Molecular Therapy?19.4 (2011): 797-804.Species: HumanSample type: Conditioned MediaIt was hard to use at first but once you get the gist of how to use it, it becomes easier, I've been using it since 2017 to detect proteases on our artificial environment and works great! Recommended.Ruston PerryNASA - Biology11/16/2019, 10:03 AMThis product is simple to use with step by step instructions. It also has multiple options for an overnight incubation, which can be beneficial on a busy day!JoehleenUC Davis12/19/2018, 03:57 PMWrite A ReviewManualSDSAnalysis Tool (QAH-ANG-2)Analysis Tool (QAH-ANG-3)