Antigen InformationGene Symbols:CSF2CXCL8IFNGIL10IL12AIL1BIL4IL6TGFB1TNFAssay FormatPathway:JAK/STAT SignalingMAPK SignalingNFkB SignalingNumber of Targets Detected:10Species Detected:Porcine (pig)Compatible Sample Types:Cell Culture SupernatantsCell LysatesOther Body FluidsPlasmaSerumTissue LysatesDesign Principle:Sandwich-basedMethod of Detection:Fluorescence Laser ScannerQuantitative/Semi-Quantitative:QuantitativeSolid Support:Glass SlideProduct SpecificationsSize:1, 2, or 4 Slide Kit (16 sub-arrays per slide)Product FeaturesMore cost-effective than traditional ELISAHigh specificity and system reproducibilitySuitable for diverse sample typesLow sample volume requirement: 50 µL or lessGet results same day (6-hour processing time)Well-suited for high throughput assaysQ Analyzer software provides one-step computationTarget NamesScroll over each target protein for more informationGM-CSFGene Symbol: CSF2Accession #: Q29118ELISA Kit: RayBio Porcine GM-CSF ELISAIFN-gammaGene Symbol: IFNGAccession #: P17803ELISA Kit: RayBio Porcine IFN-gamma ELISAIL-1 beta(IL-1 F2)Gene Symbol: IL1BAccession #: P26889ELISA Kit: RayBio Porcine IL-1 beta ELISAIL-10Gene Symbol: IL10Accession #: Q29055ELISA Kit: RayBio Porcine IL-10 ELISAIL-12 p70Gene Symbol: IL12AAccession #: Q29053ELISA Kit: RayBio Porcine IL-12 ELISAIL-4Gene Symbol: IL4Accession #: Q04745ELISA Kit: RayBio Porcine IL-4 ELISAIL-6Gene Symbol: IL6Accession #: P26893ELISA Kit: RayBio Porcine IL-6 ELISAIL-8(CXCL8)Gene Symbol: IL8Accession #: P26894ELISA Kit: RayBio Porcine IL-8 ELISATGF beta 1Gene Symbol: TGFB1Accession #: P07200ELISA Kit: RayBio Porcine TGF-beta-1 ELISATNF alphaGene Symbol: TNFAccession #: P23563ELISA Kit: RayBio Porcine TNF-alpha ELISAApplication NotesSuggested ApplicationMultiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal RangeKit ComponentsPorcine Cytokine Array Q1 Slide(s)Blocking BufferWash Buffer 1Wash Buffer 2Lyophilized Standard MixBiotinylated Detection Antibody CocktailStreptavidin-Conjugated FluorSlide Washer/DryerAdhesive Plastic StripsManualOther Materials RequiredDistilled or deionized waterSmall plastic boxes or containersPipettors, pipette tips and other common lab consumablesOrbital shaker or oscillating rockerAluminum foilGene microarray scanner or similar laser fluorescence scanner View Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn MoreProtocol OutlineDry the glass slidePrepare StandardsBlock array surfaceIncubate with Samples and StandardsIncubate with Biotinylated Detection Antibody CocktailIncubate with Streptavidin-Conjugated FluorDisassemble the glass slideScan with a gene microarray laser scannerPerform densitometry and analysisStorage/StabilityFor best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.Sohn EH., Jiao C., Kaalberg E., et al. Allogenic iPSC-derived RPE cell transplants induce immune response in pigs: a pilot study. Sci Rep. 2015 Jul 3;5:11791. doi: 10.1038/srep11791.Species: PorcineSample type: Vitreous FluidGoetz J., Fredericks D., Petersen E., et al. A clinically realistic large animal model of intra-articular fracture that progresses to post-traumatic osteoarthritis. Osteoarthritis Cartilage. 2015 Oct;23(10):1797-805. doi: 10.1016/j.joca.2015.05.022Species: PorcineSample type: Synovial FluidBeltran-Beck B., de la Fuente J., Garrido J., Aranaz A., Sevilla I., et al. Oral Vaccination with Heat Inactivated Mycobacterium bovis Activates the Complement System to Protect against Tuberculosis. PLOS One, 2014 May 19, DOI: 10.1371/journal.pone.0098048Species: PorcineSample type: SerumBeltran-Beck B., Romero B., Boadella M., et al. Tonsils of the Soft Palate Do Not Mediate the Response of Pigs to Oral Vaccination with Heat-Inactivated Mycobacterium bovis. Clin Vaccine Immunol. 2014 Aug;21(8):1128-36. doi: 10.1128/CVI.00221-14Species: PorcineSample type: SerumButz D., et al. The expired breath carbon delta value is a marker for the onset of sepsis in a swine model. Journal of Analytical Atomic Spectrometry. 2014, 29, 606-613. DOI: 10.1039/C3JA50340BSpecies: PorcineSample type: PlasmaPazos, M. R., Mohammed, N., Lafuente, H., Santos, M., Martínez-Pinilla, E., Moreno, E., ... & Hillard, C. J. (2013). Mechanisms of cannabidiol neuroprotection in hypoxic?ischemic newborn pigs: Role of 5HT 1A and CB2 receptors.?Neuropharmacology,?71, 282-291.Species: PorcineSample type: Tissue LysateMulloy DP, Stone ML, Crosby IK, et al.?Ex Vivo?Rehabilitation of Non-Heart-Beating Donor Lungs in a Preclinical Porcine Model: Delayed Perfusion Results in Superior Lung Function.?The Journal of thoracic and cardiovascular surgery. 2012;144(5):1208-1216. doi:10.1016/j.jtcvs.2012.07.056.Species: PorcineSample type: BAL FluidSensitive and robust with good reproducibility. We took advantage of the free scanning services as well. Low price in comparison to others in the market.Mallesh KurakulaUniversity of Memphis11/15/2019, 05:25 PMWrite A ReviewManualSDSGALAnalysis Tool