Antigen InformationGene Symbols:CCL2CXCL8IFNGIL10IL13IL1AIL1BIL4IL6TNFAssay FormatPathway:JAK/STAT SignalingMAPK SignalingNFkB SignalingNumber of Targets Detected:10Species Detected:HumanCompatible Sample Types:Cell Culture SupernatantsCell LysatesOther Body FluidsPlasmaSerumTissue LysatesDesign Principle:Sandwich-basedMethod of Detection:Fluorescence Laser ScannerQuantitative/Semi-Quantitative:QuantitativeSolid Support:Glass SlideProduct SpecificationsSize:1, 2, or 4 Slide Kit (16 sub-arrays per slide)Product FeaturesMore cost-effective than traditional ELISAHigh specificity and system reproducibilitySuitable for diverse sample typesLow sample volume requirement: 50 µL or lessGet results same day (6-hour processing time)Well-suited for high throughput assaysQ Analyzer software provides one-step computationTarget NamesScroll over each target protein for more informationIFN-gammaGene Symbol: IFNGGene ID: 3458Accession #: P01579ELISA Kit: RayBio Human IFN-gamma ELISAIL-1 alpha(IL-1 F1)Gene Symbol: IL1AGene ID: 3552Accession #: P01583ELISA Kit: RayBio Human IL-1 alpha ELISAIL-1 beta(IL-1 F2)Gene Symbol: IL1BGene ID: 3553Accession #: P01584ELISA Kit: RayBio Human IL-1 beta ELISAIL-10Gene Symbol: IL10Gene ID: 3586Accession #: P22301ELISA Kit: RayBio Human IL-10 ELISAIL-13Gene Symbol: IL13Gene ID: 3596Accession #: P35225ELISA Kit: RayBio Human IL-13 ELISAIL-4Gene Symbol: IL4Gene ID: 3565Accession #: P05112ELISA Kit: RayBio Human IL-4 ELISAIL-6Gene Symbol: IL6Gene ID: 3569Accession #: P05231ELISA Kit: RayBio Human IL-6 ELISAIL-8(CXCL8)Gene Symbol: IL8Gene ID: 3576Accession #: P10145ELISA Kit: RayBio Human IL-8 ELISAMCP-1(CCL2)Gene Symbol: CCL2Gene ID: 6347Accession #: P13500ELISA Kit: RayBio Human MCP-1 ELISATNF alphaGene Symbol: TNFGene ID: 7124Accession #: P01375ELISA Kit: RayBio Human TNF-alpha ELISAApplication NotesSuggested ApplicationMultiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal RangeKit ComponentsHuman Inflammation Array Q1 Slide(s)Blocking BufferWash Buffer 1Wash Buffer 2Lyophilized Standard MixBiotinylated Detection Antibody CocktailStreptavidin-Conjugated FluorSlide Washer/DryerAdhesive Plastic StripsManualOther Materials RequiredDistilled or deionized waterSmall plastic boxes or containersPipettors, pipette tips and other common lab consumablesOrbital shaker or oscillating rockerAluminum foilGene microarray scanner or similar laser fluorescence scannerView Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn MoreProtocol OutlineDry the glass slidePrepare StandardsBlock array surfaceIncubate with Samples and StandardsIncubate with Biotinylated Detection Antibody CocktailIncubate with Streptavidin-Conjugated FluorDisassemble the glass slideScan with a gene microarray laser scannerPerform densitometry and analysisStorage/StabilityFor best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.Zhang C, Ding H, He M, Liu L, Liu L, Li G, et al. (2016) Comparison of Early Changes in Ocular Surface and Inflammatory Mediators between Femtosecond Lenticule Extraction and Small-Incision Lenticule Extraction. PLoS ONE 11(3): e0149503. doi:10.1371/journal.pone.0149503Species: HumanSample type: TearsWu B., Wang W., Zhan Y., et al. CXCL13, CCL4, and sTNFR as circulating inflammatory cytokine markers in primary and SLE-related autoimmune hemolytic anemia. J Transl Med. 2015 Apr 8;13:112. doi: 10.1186/s12967-015-0474-4.Species: HumanSample type: Plasma Modulation of Cytokine Release and Gene Expression by the Immunosuppressive Domain of gp41 of HIV-1Species: HumanSample type: N/AProvençal, Nadine, et al. "Childhood chronic physical aggression associates with adult cytokine levels in plasma."?PLoS One?8.7 (2013): e69481.Species: HumanSample type: PlasmaProvençal, Nadine, et al. "Differential DNA methylation regions in cytokine and transcription factor genomic loci associate with childhood physical aggression."?PLoS One?8.8 (2013): e71691.Species: HumanSample type: PlasmaZhai Y, Zhong Z, Chen C-YA, Xia Z, Song L, Blackburn MR, Shyu A-B. Coordinated Changes in mRNA Turnover, Translation, and RNA Processing Bodies in Bronchial Epithelial Cells following Inflammatory Stimulation. Mol Cell Biol. 2008; 28(24):7414-7426.Species: HumanSample type: N/ANo posts foundWrite A ReviewManualSDSGALAnalysis Tool