Antigen InformationGene Symbols:CCL20 | CSF2 | IFNG | IFNL2 | IL10 | IL12A | IL13 | IL17A | IL17F | IL1B| IL2 | IL21 | IL22 | IL23A | IL4 | IL5 | IL6 | LTA | TGFB1 | TNF View more HideAssay FormatNumber of Targets Detected:20Species Detected:HumanCompatible Sample Types:Cell Culture SupernatantsCell LysatesOther Body FluidsPlasmaSerumTissue LysatesDesign Principle:Sandwich-basedMethod of Detection:Fluorescence Laser ScannerQuantitative/Semi-Quantitative:QuantitativeSolid Support:Glass SlideProduct SpecificationsSize:1, 2, or 4 Slide Kit (16 sub-arrays per slide)Product FeaturesMore cost-effective than traditional ELISAHigh specificity and system reproducibilitySuitable for diverse sample typesLow sample volume requirement: 50 µL or lessGet results same day (6-hour processing time)Well-suited for high throughput assaysQ Analyzer software provides one-step computationTarget NamesScroll over each target protein for more informationGM-CSFGene Symbol: CSF2Gene ID: 1437Accession #: P04141ELISA Kit: RayBio Human GM-CSF ELISAIFN-gammaGene Symbol: IFNGGene ID: 3458Accession #: P01579ELISA Kit: RayBio Human IFN-gamma ELISAIL-1 beta(IL-1 F2)Gene Symbol: IL1BGene ID: 3553Accession #: P01584ELISA Kit: RayBio Human IL-1 beta ELISAIL-10Gene Symbol: IL10Gene ID: 3586Accession #: P22301ELISA Kit: RayBio Human IL-10 ELISAIL-12 p70Gene Symbol: IL12AGene ID: 3592Accession #: P29459ELISA Kit: RayBio Human IL-12 p70 ELISAIL-13Gene Symbol: IL13Gene ID: 3596Accession #: P35225ELISA Kit: RayBio Human IL-13 ELISAIL-17AGene Symbol: IL17AGene ID: 3605Accession #: Q16552ELISA Kit: RayBio Human IL-17A ELISAIL-17FGene Symbol: IL17FGene ID: 112744Accession #: Q96PD4ELISA Kit: RayBio Human IL-17F ELISAIL-2Gene Symbol: IL2Gene ID: 3558Accession #: P60568ELISA Kit: RayBio Human IL-2 ELISAIL-21Gene Symbol: IL21Gene ID: 59067Accession #: Q9HBE4ELISA Kit: RayBio Human IL-21 ELISAIL-22Gene Symbol: IL22Gene ID: 50616Accession #: Q9GZX6ELISA Kit: RayBio Human IL-22 ELISAIL-23Gene Symbol: IL23AGene ID: 51561Accession #: Q9NPF7ELISA Kit: RayBio Human IL-23 ELISAIL-28A(IFN-lambda 2)Gene Symbol: IFNL2Gene ID: 282616Accession #: Q8IZJ0ELISA Kit: RayBio Human IL-28A ELISAIL-4Gene Symbol: IL4Gene ID: 3565Accession #: P05112ELISA Kit: RayBio Human IL-4 ELISAIL-5Gene Symbol: IL5Gene ID: 3567Accession #: P05113ELISA Kit: RayBio Human IL-5 ELISAIL-6Gene Symbol: IL6Gene ID: 3569Accession #: P05231ELISA Kit: RayBio Human IL-6 ELISAMIP-3 alpha(CCL20)Gene Symbol: CCL20Gene ID: 6364Accession #: P78556ELISA Kit: RayBio Human MIP-3 alpha ELISATGF beta 1Gene Symbol: TGFB1Gene ID: 7040Accession #: P01137ELISA Kit: RayBio Human TGF-beta 1 ELISATNF alphaGene Symbol: TNFGene ID: 7124Accession #: P01375ELISA Kit: RayBio Human TNF-alpha ELISATNF beta(TNFSF1B)Gene Symbol: LTAGene ID: 4049Accession #: P01374ELISA Kit: RayBio Human TNF-beta ELISAApplication NotesSuggested ApplicationMultiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal RangeKit ComponentsHuman Th1/Th2/Th17 Array Q1 Slide(s)Blocking BufferWash Buffer 1Wash Buffer 2Lyophilized Standard MixBiotinylated Detection Antibody CocktailStreptavidin-Conjugated FluorSlide Washer/DryerAdhesive Plastic StripsManualOther Materials RequiredDistilled or deionized waterSmall plastic boxes or containersPipettors, pipette tips and other common lab consumablesOrbital shaker or oscillating rockerAluminum foilGene microarray scanner or similar laser fluorescence scannerView Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn MoreProtocol OutlineDry the glass slidePrepare StandardsBlock array surfaceIncubate with Samples and StandardsIncubate with Biotinylated Detection Antibody CocktailIncubate with Streptavidin-Conjugated FluorDisassemble the glass slideScan with a gene microarray laser scannerPerform densitometry and analysisStorage/StabilityFor best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.Du X., Shi R., Wang Y., et al. Isoforskolin and forskolin attenuate lipopolysaccharide-induced inflammation through TLR4/MyD88/NF-?B cascades in human mononuclear leukocytes. Phytother Res. 2019 Jan 14. doi: 10.1002/ptr.6248.Species: HumanSample type: Conditioned MediaCanete J., Celis R., Yeremenko N., et al. Ectopic lymphoid neogenesis is strongly associated with activation of the IL-23 pathway in rheumatoid synovitis. Arthritis Res Ther. 2015 Jul 9;17:173. doi: 10.1186/s13075-015-0688-0.Species: HumanSample type: Synovial FluidGomez-Puerta J., et al. Differences in synovial fluid cytokine levels but not in synovial tissue cell infiltrate between anti-citrullinated peptide/protein antibody-positive and -negative rheumatoid arthritis patients. GÃ?mez-Puerta et al. Arthritis Research & Therapy 2013, 15:R182Species: HumanSample type: Synovial FluidKotlarz D, Zi?tara N, Uzel G, et al. Loss-of-function mutations in the IL-21 receptor gene cause a primary immunodeficiency syndrome. J. Exp. Med. 2013 Mar 11;210(3):433-443.Species: HumanSample type: Conditioned MediaYang P., Foster CS. Interleukin 21, interleukin 23, and transforming growth factor ?1 in HLA-A29-associated birdshot retinochoroidopathy. Am J Ophthalmol. 2013 Aug;156(2):400-406.e2. doi: 10.1016/j.ajo.2013.03.004.Species: HumanSample type: SerumGómez-Puerta, José A., et al. "Differences in synovial fluid cytokine levels but not in synovial tissue cell infiltrate between anti-citrullinated peptide/protein antibody-positive and?negative rheumatoid arthritis patients."?Arthritis research & therapy?15.6 (2013): R182.Species: HumanSample type: Synovial fluidLiu R, Wu Q, Su D, et al. A regulatory effect of IL-21 on T follicular helper-like cell and B cell in rheumatoid arthritis. Arthritis Research & Therapy. 2012;14(6):R255. doi:10.1186/ar4100.Species: HumanSample type: SerumCelis R., et al. Synovial cytokine expression in psoriatic arthritis and associations with lymphoid neogenesis and clinical features. Celis et al. Arthritis Research & Therapy 2012, 14:R93Species: HumanSample type: Synovial FluidGomez-Puerta J., et al. Differences in synovial fluid cytokine levels but not in synovial tissue cell infiltrate between anti-citrullinated peptide/protein antibody-positive and -negative rheumatoid arthritis patients. Gómez-Puerta et al. Arthritis Research & Therapy 2013, 15:R182Species: HumanSample type: Synovial FluidNo posts foundWrite A ReviewManualSDSGALAnalysis Tool