Antigen InformationGene Symbols:AGRP | ALCAM | ANG | ANGPT1 | AREG | AXL | BDNF | BMP4 | BMP5 | BMP7| BTC | CCL1 | CCL11 | CCL13 | CCL14 | CCL15 | CCL16 | CCL17 | CCL18 | CCL19 | CCL2 | CCL20 | CCL21 | CCL22 | CCL23 | CCL24 | CCL25 | CCL26 | CCL27 | CCL28 | CCL3 | CCL4 | CCL5 | CCL7 | CCL8 | CD14 | CD40 | CD40LG | CD80 | CDH1 | CEACAM1 | CNTN2 | CSF1 | CSF1R | CSF2 | CSF3 | CTSS | CXCL1 | CXCL10 | CXCL11 | CXCL12 | CXCL13 | CXCL16 | CXCL5 | CXCL6 | CXCL8 | CXCL9 | DKK1 | EDA2R | EGF | EGFR | ENG | EPCAM | ERBB3 | FAS | FASLG | FCGR2B | FGF2 | FGF4 | FGF7 | FIGF | FLT1 | FLT3LG | FLT4 | FST | GDF15 | GDNF | GH1 | HAVCR1 | HBEGF | HGF | ICAM1 | ICAM2 | ICAM3 | IFNG | IFNL1 | IFNL2 | IGF1 | IGFBP1 | IGFBP2 | IGFBP3 | IGFBP4 | IGFBP6 | IL10 | IL10RB | IL11 | IL12A | IL12B | IL13 | IL13RA1 | IL13RA2 | IL15 | IL16 | IL17A | IL17B | IL17F | IL17RA | IL18BP | IL1A | IL1B | IL1R1 | IL1RL1 | IL1RN | IL2 | IL21R | IL23A | IL2RA | IL2RB | IL2RG | IL31 | IL4 | IL5 | IL6 | IL6R | IL6ST | IL7 | IL9 | INHBA | INS | KDR | KIT | KITLG | LCN2 | LGALS7 | LIF | LTA | LYVE1 | MICA | MICB | MIF | MOK | MST1 | NGF | NGFR | NRCAM | NRG1 | NTF3 | NTF4 | PARN | PDGFA | PDGFB | PDGFRB | PECAM1 | PF4 | PGF | PI3 | PLAUR | PLG | PPBP | PROK1 | RETN | SCARB2 | SELE | SELL | SERPINE1 | SHH | SIGLEC5 | SPP1 | TDGF1 | TEK | TGFA | TGFB1 | TGFB2 | TGFB3 | THPO | TIMP1 | TIMP2 | TNF | TNFRSF10C | TNFRSF10D | TNFRSF11B | TNFRSF14 | TNFRSF17 | TNFRSF18 | TNFRSF1A | TNFRSF1B | TNFRSF21 | TNFRSF8 | TNFRSF9 | TNFSF14 | TREM1 | TSLP | TYRO3 | VCAM1 | VEGFA | VEGFC | XCL1 View more Hide Assay FormatNumber of Targets Detected:200Species Detected:HumanCompatible Sample Types:Cell Culture SupernatantsCell LysatesOther Body FluidsPlasmaSerumTissue LysatesDesign Principle:Sandwich-basedMethod of Detection:Fluorescence Laser ScannerQuantitative/Semi-Quantitative:QuantitativeSolid Support:Glass SlideProduct SpecificationsSize:1, 2, or 4 Slide Kit (16 sub-arrays per slide)Product FeaturesMore cost-effective than traditional ELISAHigh specificity and system reproducibilitySuitable for diverse sample typesLow sample volume requirement: 250 µL or lessGet results same day (6-hour processing time)Well-suited for high throughput assaysQ Analyzer software provides one-step computationTarget Names4-1BB (TNFRSF9/CD137), 6Ckine (CCL21), Activin A, AgRP, ALCAM (CD166), Amphiregulin, Angiogenin, Angiopoietin-1, Angiostatin, AXL, BCMA, BDNF, Betacellulin (BTC), beta-NGF, bFGF, BLC (CXCL13), BMP-4, BMP-5, BMP-7, Cathepsin S, CCL28 (MEC), CD14, CD30 (TNFRSF8), CD40 (TNFRSF5), CD40 Ligand (TNFSF5), CD80 (B7-1), CEACAM-1, Contactin-2 (TAG1), Cripto-1, CTACK (CCL27), CXCL16, DAN, DKK-1, DR6 (TNFRSF21), Dtk, E-Cadherin, EGF, EGFR, EG-VEGF (PK1), ENA-78 (CXCL5), Endoglin (CD105), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), ErbB3, E-Selectin, Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), Fc gamma RIIB/C (CD32B/C), FGF-4, FGF-7 (KGF), Flt-3 Ligand, Follistatin, Galectin-7, GCP-2 (CXCL6), GCSF, GDF-15, GDNF, GITR (TNFRSF18), GM-CSF, gp130 (sgp130), GRO alpha/beta/gamma, Growth Hormone, HB-EGF, HCC-1 (CCL14), HCC-4 (CCL16), HGF, HVEM (TNFRSF14), I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), IFN-gamma, IGF-1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R4 (ST2), IL-1 Ra (IL-1 F3), IL-10, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 1 (IL-13 R1), IL-13 R alpha 2, IL-15, IL-16, IL-17 RA, IL-17A, IL-17B, IL-17F, IL-18 BP alpha, IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-21 R, IL-23, IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-31, IL-4, IL-5, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-9, Insulin, IP-10 (CXCL10), I-TAC (CXCL11), LAP/TGF beta 1 (TGF beta 1), LIF, LIGHT (TNFSF14), LIMPII, Lipocalin-2 (NGAL), L-Selectin (CD62L), Lymphotactin (XCL1), LYVE-1, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MICA, MICB, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MPIF-1 (CCL23), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NGFR (TNFRSF16), NrCAM, NRG1-beta 1 (HRG1-beta 1), NT-3, NT-4, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PAI-1, PARC (CCL18), PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PECAM-1, Platelet Factor 4 (CXCL4), PLGF, RAGE, RANTES (CCL5), Resistin, SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), Siglec-5 (CD170), Sonic Hedgehog N-Terminal (Shh-N), TARC (CCL17), TECK (CCL25), TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, Thrombopoietin (TPO), Tie-2, TIM-1 (KIM-1), TIMP-1, TIMP-2, TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), Trappin-2, TREM-1, TROP1 (EpCAM), TSLP, uPAR, VCAM-1 (CD106), VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, VEGFR3, XEDARApplication NotesSuggested ApplicationMultiplexed Protein DetectionBiomarker ScreeningIdentifying Key FactorsConfirming Biological ProcessBiomarker ValidationValidation of Antibody Array ResultsQuantitative Protein DetectionEstablishing Normal RangeKit ComponentsHuman Cytokine Array Q4000 Slide(s)Blocking BufferWash Buffer 1Wash Buffer 2Lyophilized Standard MixBiotinylated Detection Antibody CocktailStreptavidin-Conjugated FluorSlide Washer/DryerAdhesive Plastic StripsManualOther Materials RequiredDistilled or deionized waterSmall plastic boxes or containersPipettors, pipette tips and other common lab consumablesOrbital shaker or oscillating rockerAluminum foilGene microarray scanner or similar laser fluorescence scannerView Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn MoreProtocol OutlineDry the glass slidePrepare StandardsBlock array surfaceIncubate with Samples and StandardsIncubate with Biotinylated Detection Antibody CocktailIncubate with Streptavidin-Conjugated FluorDisassemble the glass slideScan with a gene microarray laser scannerPerform densitometry and analysisStorage/StabilityFor best results, ore the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months."Dillman R., Cornforth A., Nistor G., et al. Randomized phase II trial of autologous dendritic cell vaccines versus autologous tumor cell vaccines in metastatic melanoma: 5-year follow up and additional analyses. J Immunother Cancer. 2018 Mar 6;6(1):19. doi: 10.1186/s40425-018-0330-1.Species: HumanSample type: SerumVelez G., Bassuk A., Colgan D., et al. Therapeutic drug repositioning using personalized proteomics of liquid biopsies. JCI Insight. 2017 Dec 21;2(24). pii: 97818. doi: 10.1172/jci.insight.97818.Species: HumanSample type: Vitreous FluidVelez G., Roybal C., Colgan D., et al. Precision Medicine: Personalized Proteomics for the Diagnosis and Treatment of Idiopathic Inflammatory Disease. JAMA Ophthalmol. 2016 Apr;134(4):444-8. doi: 10.1001/jamaophthalmol.2015.5934.Species: HumanSample type: Vitreous FluidMartinaud C., Desterke C., Konopacki J., et al. Osteogenic Potential of Mesenchymal Stromal Cells Contributes to Primary Myelofibrosis. Cancer Res. 2015 Nov 15;75(22):4753-65. doi: 10.1158/0008-5472.CAN-14-3696Species: HumanSample type: Conditioned MediaWe had RayBiotech run this panel on a collection of human brain samples. The results are terrific and working with Raybiotech has been great.Katy Murphy01/25/2018, 01:50 PMWrite A ReviewManualSDSAnalysis Tool (QAH-INF-3)Analysis Tool (QAH-GF-1)Analysis Tool (QAH-CHE-1)Analysis Tool (QAH-REC-1)Analysis Tool (QAH-CYT-4)