Antigen InformationGene Symbols:CCL2 | CCL3 | CCL4 | CCL5 | CSF2 | CXCL1 | CXCL2 | CXCL3 | CXCL8 | IFNG| IL10 | IL12A | IL13 | IL1A | IL1B | IL2 | IL4 | IL5 | IL6 | MMP9 | TNF | VEGFA View more HideAssay FormatNumber of Targets Detected:20Species Detected:Human Compatible Sample Types:Cell Culture SupernatantsCell LysatesOther Body FluidsPlasmaSerumTissue LysatesDesign Principle:Sandwich-basedMethod of Detection:Fluorescence Laser ScannerQuantitative/Semi-Quantitative:QuantitativeSolid Support:Glass SlideProduct SpecificationsSize:1, 2, or 4 Slide Kit (16 sub-arrays per slide)Product FeaturesMore cost-effective than traditional ELISAHigh specificity and system reproducibilitySuitable for diverse sample typesLow sample volume requirement: 50 µL or lessGet results same day (6-hour processing time)Well-suited for high throughput assaysQ Analyzer software provides one-step computationTarget NamesScroll over each target protein for more informationGM-CSFGene Symbol: CSF2Gene ID: 1437Accession #: P04141ELISA Kit: RayBio Human GM-CSF ELISAGRO alpha/beta/gammaGene Symbol: CXCL1/CXCL2/CXCL3Gene ID: 2919/2920/2921Accession #: P09341/P19875/P19876IFN-gammaGene Symbol: IFNGGene ID: 3458Accession #: P01579ELISA Kit: RayBio Human IFN-gamma ELISAIL-1 alpha(IL-1 F1)Gene Symbol: IL1AGene ID: 3552Accession #: P01583ELISA Kit: RayBio Human IL-1 alpha ELISAIL-1 beta(IL-1 F2)Gene Symbol: IL1BGene ID: 3553Accession #: P01584ELISA Kit: RayBio Human IL-1 beta ELISAIL-10Gene Symbol: IL10Gene ID: 3586Accession #: P22301ELISA Kit: RayBio Human IL-10 ELISAIL-12 p70Gene Symbol: IL12AGene ID: 3592Accession #: P29459ELISA Kit: RayBio Human IL-12 p70 ELISAIL-13Gene Symbol: IL13Gene ID: 3596Accession #: P35225ELISA Kit: RayBio Human IL-13 ELISAIL-2Gene Symbol: IL2Gene ID: 3558Accession #: P60568ELISA Kit: RayBio Human IL-2 ELISAIL-4Gene Symbol: IL4Gene ID: 3565Accession #: P05112ELISA Kit: RayBio Human IL-4 ELISAIL-5Gene Symbol: IL5Gene ID: 3567Accession #: P05113ELISA Kit: RayBio Human IL-5 ELISAIL-6Gene Symbol: IL6Gene ID: 3569Accession #: P05231ELISA Kit: RayBio Human IL-6 ELISAIL-8(CXCL8)Gene Symbol: IL8Gene ID: 3576Accession #: P10145ELISA Kit: RayBio Human IL-8 ELISAMCP-1(CCL2)Gene Symbol: CCL2Gene ID: 6347Accession #: P13500ELISA Kit: RayBio Human MCP-1 ELISAMIP-1 alpha(CCL3)Gene Symbol: CCL3Gene ID: 6348Accession #: P10147ELISA Kit: RayBio Human MIP-1 alpha ELISAMIP-1 beta(CCL4)Gene Symbol: CCL4Gene ID: 6351Accession #: P13236ELISA Kit: RayBio Human MIP-1 beta ELISAMMP-9Gene Symbol: MMP9Gene ID: 4318Accession #: P14780ELISA Kit: RayBio Human MMP-9 ELISARANTES(CCL5)Gene Symbol: CCL5Gene ID: 6352Accession #: P13501ELISA Kit: RayBio Human RANTES ELISATNF alphaGene Symbol: TNFGene ID: 7124Accession #: P01375ELISA Kit: RayBio Human TNF-alpha ELISAVEGF-AGene Symbol: VEGFAGene ID: 7422Accession #: P15692ELISA Kit: RayBio Human VEGF-A ELISAApplication NotesSuggested ApplicationMultiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal RangeKit ComponentsHuman Cytokine Array Q1 Slide(s)Blocking BufferWash Buffer 1Wash Buffer 2Lyophilized Standard MixBiotinylated Detection Antibody CocktailStreptavidin-Conjugated FluorSlide Washer/DryerAdhesive Plastic StripsManualOther Materials RequiredDistilled or deionized waterSmall plastic boxes or containersPipettors, pipette tips and other common lab consumablesOrbital shaker or oscillating rockerAluminum foilGene microarray scanner or similar laser fluorescence scannerView Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn MoreProtocol OutlineDry the glass slidePrepare StandardsBlock array surfaceIncubate with Samples and StandardsIncubate with Biotinylated Detection Antibody CocktailIncubate with Streptavidin-Conjugated FluorDisassemble the glass slideScan with a gene microarray laser scannerPerform densitometry and analysisStorage/StabilityFor best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.Chen T., Li H., Lin J., et al. Profiling the human immune response to Mycobacterium tuberculosis by human cytokine array. Tuberculosis (Edinb). 2016 Mar;97:108-17. doi: 10.1016/j.tube.2015.12.007. Species: HumanSample type: SerumYang J., Fang P., Yu D., et al. Chronic Kidney Disease Induces Inflammatory CD40+ Monocyte Differentiation via Homocysteine Elevation and DNA Hypomethylation. Circ Res. 2016 Nov 11;119(11):1226-1241.Species: HumanSample type: SerumChen S., Zha X., Shi L., et al. Upregulated TCR? improves cytokine secretion in T cells from patients with AML. J Hematol Oncol. 2015; 8: 72. Published online 2015 Jun 18. doi: 10.1186/s13045-015-0170-0Species: HumanSample type: Conditioned MediaZhan Y., Zou S., Hua F., Li F., Ji L., Wang W., Ye Y., Sun L., Chen H., Cheng Y. High-dose dexamethasone modulates serum cytokine profile in patients with primary immune thrombocytopenia. Immunology Letters Volume 160, Issue 1, July 2014, Pages 33-38Species: HumanSample type: SerumWu H., et al. Upregulation of innate immune responses in a T cell/histiocyte-rich large B cell lymphoma patient with significant autoimmune disorders mimicking systemic lupus erythematosus. Annals of Hematology (2014) 93:353-354Species: HumanSample type: PlasmaJiang, Weidong, Ruo-Pan Huang, and Ruochun Huang. "Protein Expression Profiling by Antibody Array Analysis with Use of Dried Blood Spot Samples on Filter Paper." Journal of Immunological Methods. ScienceDirect, 25 Nov. 2013. Web.Species: HumanSample type: SerumKrÃ?nkel, Nicolle, et al. "Novel insights into the critical role of bradykinin and the kinin B2 receptor for vascular recruitment of circulating endothelial repair-promoting mononuclear cell subsets: alterations in patients with coronary disease."?Circulation?(2012): CIRCULATIONAHA-112.Species: HumanSample type: Conditioned MediaLÃ?nne, M., Lavrentieva, A., Walter, J. G., & Kasper, C. (2013). Analysis of oxygen-dependent cytokine expression in human mesenchymal stem cells derived from umbilical cord.?Cell and tissue research,?353(1), 117-122.Species: HumanSample type: Cell LysateLogan, Patrick, Julia Burnier, and Miguel N. Burnier Jr. "Vascular endothelial growth factor expression and inhibition in uveal melanoma cell lines."?ecancermedicalscience?7 (2013).Species: HumanSample type: Conditioned MediaVarnum S., Springer D., Chaffee M., et al. The effects of low-dose irradiation on inflammatory response proteins in a 3D reconstituted human skin tissue model. Radiat Res. 2012 Dec;178(6):591-9. doi: 10.1667/RR2976.1.Species: HumanSample type: Tissue LysatePrev12NextIt's user friendly and worked really well for me. KrisUniversity of Colorado Anschutz Medical School02/12/2020, 01:32 PMThis kit has been used to quantify the cytokines in the serum from patients in the following paper: Circ Res. 2016 Nov 11;119(11):1226-1241. This assay is very sensitive.Ji Yeon YangTemple University08/13/2019, 10:13 AMDirections easy to follow and they provide free scanning services. I also recommend the software.James BardillUniversity of Colorado Anschutz Medical Campus06/04/2019, 07:26 PMDefinitely one of the better cytokine arrays I've worked with.Kinan AlhallakWashington University in St. Louis01/18/2019, 01:50 PMInstructions were very clear and straightforward; it was a relatively simple process for a first-time user. Plenty of buffers and diluents included, and free analyzing by Ray-biotech if you don't have a compatible scanner accessible. Amanda JeskeWashington University in St. Louis09/10/2018, 04:32 PMWrite A ReviewManualSDSGALAnalysis Tool