Antigen InformationGene Symbols:CCL20 | IFNG | IFNL2 | IL10 | IL12A | IL13 | IL17A | IL17F | IL1B | IL2| IL21 | IL22 | IL23A | IL4 | IL5 | IL6 | TGFB1 | TNF View more HideAssay FormatNumber of Targets Detected:18Species Detected:MouseCompatible Sample Types:Cell Culture SupernatantsCell LysatesOther Body FluidsPlasmaSerumTissue LysatesDesign Principle:Sandwich-basedMethod of Detection:Fluorescence Laser ScannerQuantitative/Semi-Quantitative:QuantitativeSolid Support:Glass SlideProduct SpecificationsSize:1, 2, or 4 Slide Kit (16 sub-arrays per slide)Product FeaturesMore cost-effective than traditional ELISAHigh specificity and system reproducibilitySuitable for diverse sample typesLow sample volume requirement: 50 µL or lessGet results same day (6-hour processing time)Well-suited for high throughput assaysQ Analyzer software provides one-step computationTarget NamesScroll over each target protein for more informationIFN-gammaGene Symbol: IFNGAccession #: P01580ELISA Kit: RayBio Mouse IFN-gamma ELISAIL-1 beta(IL-1 F2)Gene Symbol: IL1BAccession #: P10749ELISA Kit: RayBio Mouse IL-1 beta ELISAIL-10Gene Symbol: IL10Accession #: Q0VBJ1ELISA Kit: RayBio Mouse IL-10 ELISAIL-12 p70Gene Symbol: IL12AAccession #: P43431IL-13Gene Symbol: IL13Accession #: P20109ELISA Kit: RayBio Mouse IL-13 ELISAIL-17AGene Symbol: IL17AAccession #: Q60971ELISA Kit: RayBio Mouse IL-17A ELISAIL-17FGene Symbol: IL17FAccession #: Q7TNI7ELISA Kit: RayBio Mouse IL-17F ELISAIL-2Gene Symbol: IL2Accession #: Q791T3ELISA Kit: RayBio Mouse IL-2 ELISAIL-21Gene Symbol: IL21Accession #: E9PX58ELISA Kit: RayBio Mouse IL-21 ELISAIL-22Gene Symbol: IL22Accession #: Q14BB3ELISA Kit: RayBio Mouse IL-22 ELISAIL-23 p19Gene Symbol: IL23AAccession #: Q9EQ14ELISA Kit: RayBio Mouse IL-23 alpha p19 ELISAIL-28A(IFN-lambda 2)Gene Symbol: IFNL2Accession #: Q4VK74ELISA Kit: RayBio Mouse IL-28 ELISAIL-4Gene Symbol: IL4Accession #: P07750ELISA Kit: RayBio Mouse IL-4 ELISAIL-5Gene Symbol: IL5Accession #: P04401ELISA Kit: RayBio Mouse IL-5 ELISAIL-6Gene Symbol: IL6Accession #: P08505ELISA Kit: RayBio Mouse IL-6 ELISAMIP-3 alpha(CCL20)Gene Symbol: CCL20Accession #: O89093ELISA Kit: RayBio Mouse MIP-3 alpha ELISATGF beta 1Gene Symbol: TGFB1Accession #: P04202TNF alphaGene Symbol: TNFAccession #: Q62326ELISA Kit: RayBio Mouse TNF-alpha ELISAApplication NotesSuggested ApplicationMultiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal RangeKit ComponentsMouse Th1/Th2/Th17 Array Q2 Slide(s)Blocking BufferWash Buffer 1Wash Buffer 2Lyophilized Standard MixBiotinylated Detection Antibody CocktailStreptavidin-Conjugated FluorSlide Washer/DryerAdhesive Plastic StripsManualOther Materials RequiredDistilled or deionized waterSmall plastic boxes or containersPipettors, pipette tips and other common lab consumablesOrbital shaker or oscillating rockerAluminum foilGene microarray scanner or similar laser fluorescence scannerView Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn MoreProtocol OutlineDry the glass slidePrepare StandardsBlock array surfaceIncubate with Samples and StandardsIncubate with Biotinylated Detection Antibody CocktailIncubate with Streptavidin-Conjugated FluorDisassemble the glass slideScan with a gene microarray laser scannerPerform densitometry and analysisStorage/StabilityFor best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.Velsko I., Chukkapalli S., Rivera-Kweh M., et al. Periodontal pathogens invade gingiva and aortic adventitia and elicit inflammasome activation in ?v?6 integrin-deficient mice. Infect Immun. 2015 Dec;83(12):4582-93. doi: 10.1128/IAI.01077-15.Species: HumanSample type: Conditioned MediaChe N. et al. Impaired B cell Inhibition by Lupus Bone Marrow Mesenchymal Stem Cells is Caused by Reduced CCL2 Expression. J Immunol 2014; 193:5306-5314; Prepublished online 22. doi: 10.4049/jimmunol.1400036Species: MouseSample type: Conditioned mediaHou T., Li Z., Luo F., Xie Z., Wu X., Xing J., Dong S., Xu J. A composite demineralized bone matrix e Self assembling peptide scaffold for enhancing cell and growth factor activity in bone marrow. Biomaterials, Available online 19 April 2014. [Epub ahead of print]Species: MouseSample type: Tissue LysateHou, Ni, et al. "A novel chronic stress-induced shift in the Th1 to Th2 response promotes colon cancer growth."?Biochemical and biophysical research communications?439.4 (2013): 471-476.Species: MouseSample type: SerumMarchesan, Julie Teresa, et al. "Porphyromonas gingivalis oral infection exacerbates the development and severity of collagen-induced arthritis."?Arthritis research & therapy?15.6 (2013): R186.Species: MouseSample type: SerumAbdi K., Sing N., Matzinger P. Lipopolysaccharide-activated dendritic cells: "exhausted" or alert and waiting? J Immunol. 2012 Jun 15;188(12):5981-9. doi: 10.4049/jimmunol.1102868. Species: MouseSample type: Conditioned MediaNo posts foundWrite A ReviewManualSDSGALAnalysis Tool