Antigen InformationGene Symbols:CCL2IFNGIL10IL13IL1AIL1BIL2IL4IL6TNFAssay FormatPathway:JAK/STAT SignalingMAPK SignalingNFkB SignalingNumber of Targets Detected:10Species Detected:RatCompatible Sample Types:Cell Culture SupernatantsCell LysatesOther Body FluidsPlasmaSerumTissue LysatesDesign Principle:Sandwich-basedMethod of Detection:Fluorescence Laser ScannerQuantitative/Semi-Quantitative:Quantitative Solid Support:Glass SlideProduct SpecificationsSize:1, 2, or 4 Slide Kit (16 sub-arrays per slide)Product FeaturesMore cost-effective than traditional ELISAHigh specificity and system reproducibilitySuitable for diverse sample typesLow sample volume requirement: 50 µL or lessGet results same day (6-hour processing time)Well-suited for high throughput assaysQ Analyzer software provides one-step computationTarget NamesScroll over each target protein for more informationIFN-gammaGene Symbol: IFNGAccession #: P01581ELISA Kit: RayBio Rat IFN-gamma ELISAIL-1 alpha(IL-1 F1)Gene Symbol: IL1AAccession #: P16598ELISA Kit: RayBio Rat IL-1 alpha ELISAIL-1 beta(IL-1 F2)Gene Symbol: IL1BAccession #: Q63264ELISA Kit: RayBio Rat IL-1 beta ELISAIL-2Gene Symbol: IL2Accession #: P17108ELISA Kit: RayBio Rat IL-2 ELISAIL-4Gene Symbol: IL4Accession #: P20096ELISA Kit: RayBio Rat IL-4 ELISAIL-6Gene Symbol: IL6Accession #: P20607ELISA Kit: RayBio Rat IL-6 ELISAIL-10Gene Symbol: IL10Accession #: P29456ELISA Kit: RayBio Rat IL-10 ELISAIL-13Gene Symbol: IL13Accession #: P42203ELISA Kit: RayBio Rat IL-13 ELISA (For Lysates)MCP-1(CCL2)Gene Symbol: CCL2Accession #: P14844ELISA Kit: RayBio Rat MCP-1 ELISATNF alphaGene Symbol: TNFAccession #: P16599ELISA Kit: RayBio Rat TNF-alpha ELISAApplication NotesSuggested ApplicationMultiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal RangeKit ComponentsRat Inflammation Array Q1 Slide(s)Blocking BufferWash Buffer 1Wash Buffer 2Lyophilized Standard MixBiotinylated Detection Antibody CocktailStreptavidin-Conjugated FluorSlide Washer/DryerAdhesive Plastic StripsManualOther Materials RequiredDistilled or deionized waterSmall plastic boxes or containersPipettors, pipette tips and other common lab consumablesOrbital shaker or oscillating rockerAluminum foilGene microarray scanner or similar laser fluorescence scannerView Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn MoreProtocol OutlineDry the glass slidePrepare StandardsBlock array surfaceIncubate with Samples and StandardsIncubate with Biotinylated Detection Antibody CocktailIncubate with Streptavidin-Conjugated FluorDisassemble the glass slideScan with a gene microarray laser scannerPerform densitometry and analysisStorage/StabilityFor best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.Bigelman E., Cohen L., Aharon-Hananel G., et al. Pathological presentation of cardiac mitochondria in a rat model for chronic kidney disease. PLoS One. 2018 Jun 11;13(6):e0198196. doi: 10.1371/journal.pone.0198196Species: RatSample type: PlasmaZheng S., Pan Y., Wang C., et al. HMGB1 Turns Renal Tubular Epithelial Cells into Inflammatory Promoters by Interacting with TLR4 During Sepsis. J Interferon Cytokine Res. 2016 Jan;36(1):9-19. doi: 10.1089/jir.2015.0067. Species: RatSample type: Conditioned MediaChang Y., Jia X., Sun X., et al. APRIL promotes proliferation, secretion and invasion of fibroblast-like synoviocyte from rats with adjuvant induced arthritis. Mol Immunol. 2015 Mar;64(1):90-8. doi: 10.1016/j.molimm.2014.11.002.Species: RatSample type: Conditioned Media Jiang S., Zhang M., Tian Z., et al. The monoacylglycerol lipase inhibitor JZL184 decreases inflammatory response in skeletal muscle contusion in rats. Eur J Pharmacol. 2015 Aug 15;761:1-10. doi: 10.1016/j.ejphar.2015.04.018.Species: RatSample type: Tissue LysateZhang M., Jiang S., Tian Z., et al. CB2R orchestrates fibrogenesis through regulation of inflammatory response during the repair of skeletal muscle contusion. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3491-502. eCollection 2015Species: RatSample type: Tissue LysateWillett N., et al. Intra-articular injection of micronized dehydrated human amnion/chorion membrane attenuates osteoarthritis development. Arthritis Res Ther. 2014;16: R47.Species: RatSample type: Synovial FluidGreat kit and easy to usevschaal80UNMC05/12/2020, 04:04 PMGoodSneham Tiwariunmc05/12/2020, 04:03 PMThis array was very easy to use and provided great results for my project. The only downside was I did not have the appropriate scanner but the company offers complimentary scanning.ECUniversity of Florida02/03/2020, 09:11 PMWrite A ReviewManualSDSGALAnalysis Tool