Antigen InformationGene Symbols:MMP1MMP10MMP13MMP2MMP3MMP8MMP9TIMP1TIMP2TIMP4Assay FormatPathway:Akt SignalingHIF-1 alpha SignalingNumber of Targets Detected:10Species Detected:HumanCompatible Sample Types:Cell Culture SupernatantsCell LysatesOther Body FluidsPlasmaSerumTissue LysatesDesign Principle:Sandwich-basedMethod of Detection:Fluorescence Laser ScannerQuantitative/Semi-Quantitative:QuantitativeSolid Support: Glass SlideProduct SpecificationsSize:1, 2, or 4 Slide Kit (16 sub-arrays per slide)Product FeaturesMore cost-effective than traditional ELISAHigh specificity and system reproducibilitySuitable for diverse sample typesLow sample volume requirement: 50 µL or lessGet results same day (6-hour processing time)Well-suited for high throughput assaysQ Analyzer software provides one-step computationTarget NamesScroll over each target protein for more informationMMP-1Gene Symbol: MMP1Gene ID: 4312Accession #: P03956ELISA Kit: RayBio Human MMP-1 ELISAMMP-10Gene Symbol: MMP10Gene ID: 4319Accession #: P09238ELISA Kit: RayBio Human MMP-10 ELISAMMP-13Gene Symbol: MMP13Gene ID: 4322Accession #: P45452ELISA Kit: RayBio Human MMP-13 ELISAMMP-2Gene Symbol: MMP2Gene ID: 4313Accession #: P08253ELISA Kit: RayBio Human MMP-2 ELISAMMP-3Gene Symbol: MMP3Gene ID: 4314Accession #: P08254ELISA Kit: RayBio Human MMP-3 ELISAMMP-8Gene Symbol: MMP8Gene ID: 4317Accession #: P22894ELISA Kit: RayBio Human MMP-8 ELISAMMP-9Gene Symbol: MMP9Gene ID: 4318Accession #: P14780ELISA Kit: RayBio Human MMP-9 ELISATIMP-1Gene Symbol: TIMP1Gene ID: 7076Accession #: P01033ELISA Kit: RayBio Human TIMP-1 ELISATIMP-2Gene Symbol: TIMP2Gene ID: 7077Accession #: P16035ELISA Kit: RayBio Human TIMP-2 ELISATIMP-4Gene Symbol: TIMP4Gene ID: 7079Accession #: Q99727ELISA Kit: RayBio Human TIMP-4 ELISAApplication NotesSuggested ApplicationMultiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal RangeKit ComponentsHuman MMP Array Q1 Slide(s)Sample DiluentWash Buffer 1Wash Buffer 2Lyophilized Standard MixBiotinylated Detection Antibody CocktailStreptavidin-Conjugated FluorSlide Washer/DryerAdhesive Plastic StripsManualOther Materials RequiredDistilled or deionized waterSmall plastic boxes or containersPipettors, pipette tips and other common lab consumablesOrbital shaker or oscillating rockerAluminum foilGene microarray scanner or similar laser fluorescence scannerView Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn MoreProtocol OutlineDry the glass slidePrepare StandardsBlock array surfaceIncubate with Samples and StandardsIncubate with Biotinylated Detection Antibody CocktailIncubate with Streptavidin-Conjugated FluorDisassemble the glass slideScan with a gene microarray laser scannerPerform densitometry and analysisStorage/StabilityFor best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.Sava P., Ramanathan A., Dobronyi A., et al. Human pericytes adopt myofibroblast properties in the microenvironment of the IPF lung. JCI Insight. 2017 Dec 21;2(24). pii: 96352. doi: 10.1172/jci.insight.96352Species: HumanSample type: Conditioned MediaCekanova M., Feranando RI., Siriwardhana N., et al. BCL-2 family protein, BAD is down-regulated in breast cancer and inhibits cell invasion. Exp Cell Res. 2015 Feb 1;331(1):1-10. doi: 10.1016/j.yexcr.2014.11.016. Species: HumanSample type: Cell LysateCollazos J., Asensi V., Martin G., et al. The effect of gender and genetic polymorphisms on matrix metalloprotease (MMP) and tissue inhibitor (TIMP) plasma levels in different infectious and non-infectious conditions. Clin Exp Immunol. 2015 Nov;182(2):213-9. doi: 10.1111/cei.12686.Species: HumanSample type: PlasmaTian F., Qin C., Li X., et al. Decreased stathmin-1 expression inhibits trophoblast proliferation and invasion and is associated with recurrent miscarriage. Am J Pathol. 2015 Oct;185(10):2709-21. doi: 10.1016/j.ajpath.2015.06.010. Species: HumanSample type: Conditioned MediaCekanova M., Fernando R., Siriwardhana N., et al. BCL-2 family protein, BAD is down-regulated in breast cancer and inhibits cell invasion. Exp Cell Res. 2015 Feb 1;331(1):1-10. doi: 10.1016/j.yexcr.2014.11.016. Species: HumanSample type: Conditioned MediaChia C., Kumari U., Casey P. Breast cancer cell invasion mediated by Galpha12 signaling involves expression of interleukins-6 and -8, and matrix metalloproteinase-2. Journal of Molecular Signaling 2014, 9:6 doi:10.1186/1750-2187-9-6Species: HumanSample type: Conditioned MediaEpanchintsev A., Pavithra S., Verma R., Lyakhovich A. IL-6, IL-8, MMP-2, MMP-9 are overexpressed in Fanconi anemia cells through a NF-?B/TNF-? dependent mechanismSpecies: HumanSample type: Conditioned MediaMartin G., Asensi V., Montes H., Collazos J., Alvarez V., Carton J., Taboada F., Valle-Garay E. Role of plasma matrix-metalloproteases (MMPs) and their polymorphisms (SNPs) in sepsis development and outcome in ICU patients. Scientific Reports 4, 5002. doi:10.1038/srep05002.Species: HumanSample type: PlasmaWang Y., et al. Circulating matrix metalloproteinase patterns in association with aortic dilatation in bicuspid aortic valve patients with isolated severe aortic stenosis. Heart and Vessels, October 2014, epub ahead of print. DOI 10.1007/s00380-014-0593-5Species: HumanSample type: PlasmaYang C-C, Lin C-Y, Wang H-S, Lyu S-R (2013) Matrix Metalloproteases and Tissue Inhibitors of Metalloproteinases in Medial Plica and PannuslikeTissue Contribute to Knee Osteoarthritis Progression. PLoS ONE 8(11): e79662. doi:10.1371/journal.pone.0079662Species: HumanSample type: Tissue LysatePrev12NextThe end user in our lab had the following to say: "I would rate it 5/5. Works as described. Affordable."Kathryn GillilandHershey Medical Center08/08/2018, 05:42 AMWrite A ReviewManualSDSGALAnalysis Tool