Antigen InformationGene Symbols:CALCA | CENPA | CENPB | GARS | HARS | KRT19 | KRT20 | KRT8 | MPO | NUP210| NUP62 | PCNA | PRTN3 | SNRNP70 | SP100 | SSB | TARS2 | TG | TGM2 | THPO | TOP1 | TRIM21 View more HideAssay FormatNumber of Targets Detected:33Species Detected:HumanCompatible Sample Types:SerumDesign Principle:Sandwich-basedMethod of Detection:Fluorescence Laser ScannerQuantitative/Semi-Quantitative:Semi-QuantitativeSolid Support:Glass SlideProduct SpecificationsSize:16 Sample KitProduct FeaturesSmall sample volume required (2 µl)High density (simultaneously detect 33 autoantibodies)High sensitivityLarge dynamic rangeSuitable for high-throughput assaysHigh efficiency and accuracyAffordable, quick and simple to useCustomizable: Tell us the autoimmunogens you"re interested inTarget NameScroll over each target protein for more informationAlanyl-tRNA SynthetaseCytokeratin 8(KRT8)Gene Symbol: KRT8 CYK8Gene ID: 3856Accession #: P05787ELISA Kit: RayBio Human Cytokeratin 8/KRT8 ELISACentromere protein B(CENP-B)Gene Symbol: CENPBGene ID: 1852Accession #: P07199Centromere protein A(CENP-A)Gene Symbol: CENPAGene ID: 1851Accession #: P49450dsDNAGliadinGlycine-tRNA ligase(AP-4-A synthetase/GlyRS)Gene Symbol: GARSGene ID: 4162Accession #: P41250Nuclear pore protein gp210(POM210/Nucleoporin Nup210)Gene Symbol: NUP210 KIAA0906 PSEC0245Gene ID: 30052Accession #: Q8TEM1Histone H2AHistone H2BCytokeratin 19(KRT19)Gene Symbol: KRT19Gene ID: 3880Accession #: P08727ELISA Kit: RayBio Human Cytokeratin 19/KRT19 ELISAHistidine-tRNA ligase(HisRS)Gene Symbol: HARSGene ID: 4816Accession #: P12081Lupus La protein(SS-B)Gene Symbol: SSBGene ID: 11316Accession #: P05455LC-1Cytokeratin 20(KRT20)Gene Symbol: KRT20Gene ID: 54474Accession #: P35900ELISA Kit: RayBio Human Cytokeratin 20 ELISAM/Scl 100M2Mi-2MyeloperoxidaseGene Symbol: MPOGene ID: 4353Accession #: P05164Nuclear pore glycoprotein p62(Nucleoporin Nup62)Gene Symbol: NUP62Gene ID: 8066Accession #: P37198CalcitoninGene Symbol: CALCA CALC1Gene ID: 796Accession #: P01258ELISA Kit: RayBio Human Calcitonin ELISAPCNAGene Symbol: PCNAGene ID: 5111Accession #: P12004ELISA Kit: RayBio Human PCNA ELISAMyeloblastin(PR3/C-ANCA)Gene Symbol: PRTN3 MBNGene ID: 9495Accession #: P24158TRIM21(Ro(SS-A)/SS-A)Gene Symbol: TRIM21 RNF81 RO52 SSA1Gene ID: 11312Accession #: P19474TRIM21(Ro(SS-A)/SS-A)Gene Symbol: TRIM21 RNF81 RO52 SSA1Gene ID: 11312Accession #: P19474DNA topoisomerase 1(DNA topoisomerase I)Gene Symbol: TOP1Gene ID: 11986Accession #: P11387Sm core proteinSp-100Gene Symbol: SP100Gene ID: 11206Accession #: P23497Threonine-tRNA ligase(ThrRS)Gene Symbol: TARS2Gene ID: 30740Accession #: Q9BW92ThyroglobulinGene Symbol: TGGene ID: 7038Accession #: P01266ELISA Kit: RayBio Human Thyroglobulin ELISAThrombopoietin(TPO)Gene Symbol: THPO MGDFGene ID: 7066Accession #: P40225ELISA Kit: RayBio Human TPO ELISATransglutaminase 2(TGM2)Gene Symbol: TGM2Gene ID: 7052Accession #: P21980ELISA Kit: RayBio Human Transglutaminase 2 ELISAsnRNP70Gene Symbol: SNRNP70 RNPU1Z RPU1 SNRP70 U1AP1Gene ID: 11150Accession #: P08621  Application NotesSuggested ApplicationIgG autoantibody profiling for following common autoimmune diseases, but may not be limited to:Systemic lupus erythematosus (SLE)Sjøgren"s syndromeRheumatoid arthritisSystemic Sclerosis/CREST syndromePrimary biliary cirrhosisAutoimmune hepatitisHashimoto"s thyroiditisPolymyositis/DermatomyositisSystem vasculitisCeliac diseaseThe following table shows multiple IgG-type autoantibodies that can be detected in patient sera of different autoimmune diseases.Autoimmune DiseasesTarget No.AutoimmunogensSystemic lupus erythematosus (SLE)#5dsDNA#9Histone H2A#10Histone H2B#22Proliferating Cell Nuclear Antigen (PCNA)#26Scl-70 (DNA topoisomerase-1)#33U1-snRNP70 kDa#12Jo-1 (histidyl tRNA synthetase, histidine-tRNA ligase)#27Sm/Smith/snRNP core proteinRheumatoid arthritis#21CCP peptide#2KRT8#11KRT19#15KRT20Systemic Sclerosis / CREST Syndrome#4Centromere Protein A (CENP-A)#3Centromere B (CENP-B)#16M/Scl 100#27Sm/Smith/snRNP core proteinPrimary biliary cirrhosis#8gp-210#17M2#20Nup62#28sp100Autoimmune hepatitis#14Liver cystolic antigen type 1 (LC-1)Hashimoto"s thyroiditis#30Thyroglobulin (TG)#31Thyroid peroxidase (TPO)Polymyositis / Dermatomyositis#1Alanyl-tRNA Synthetase (PL-12)#7Glycyl-tRNA Synthetase (EJ)#18Mi-2#29Threonyl-tRNA synthetase (PL-7)#14Liver cystolic antigen type 1 (LC-1)System vasculitis#19Myeloperoxidase (MPO), p-ANCA#23Proteinase-3 (PR3), c-ANCA#3Centromere B (CENP-B)Sjøgren"s syndrome#13La/SS-B#24Ro/SS-A (52 kDa)#25Ro/SS-A (60 kDa)Celiac disease#6Gliadin#32Tissue Transglutaminase (tTG)Kit ComponentsRayBio® Human Autoimmune Disease IgG Autoantibody G1 array slideRayBio® G-series antibody array accessory (including slide incubation chamber assembly, gasket, protective cover, snap-on sides and adhesive film)Blocking Buffer (8 ml/bottle)Wash Buffer 1 (30 ml/bottle)Wash Buffer 2 (30 ml/bottle)1,000X Biotin-conjugated anti-human IgG (1.5 µl/1 vial)1,500X Streptavidin-Conjugated Fluor (1 µl/vial)Other Materials RequiredSmall plastic boxes or containersOrbital shaker or oscillating rockerPipettors, pipette tips and other common lab consumablesDistilled waterAluminum foilLaser scanner for fluorescence detectionProtocol OutlineSee Manual for relevant protocols.Below is the quick protocol for the experienced users.Typical DataThe following Figure 1 represents a typical result using serum on the RayBio® Autoimmune Disease IgG Autoantibody Detection Array (Cat #. PAH-AIDG-G1). The protein arrays were incubated with 200-fold diluted serum samples from normal individuals and patients with different autoimmune diseases. After washing steps, the arrays were sequentially incubated with biotin-labeled anti-human IgG antibody and fluorescent dye-labeled streptavidin. The signals from the bound autoantibodies were captured with a fluorescence scanner.Sample diluent alone (Figs. 1A & 1B) and normal sera (Figs. 1C & 1D) were used as negative controls, and the arrays signals indicate that no significant background occurred. In some cases, a negligible signal can be observed, which may be caused by non-specific binding derived from the biotin-labeled anti-human IgG antibody and/or fluorescent dye-labeled streptavidin. However, these weak background signals will be subtracted in subsequent data analysis procedures and will not affect sample test results. A strong binding signal was detected in multiple targets among different autoimmune disease patients: systemic lupus erythematosus (Figs.1E & 1F), Sjøgren"s syndrome (Figs.1G & 1H), system vasculitis (Figs.1I & 1J), and primary biliary cirrhosis (Figs.1K & 1L).Fig 1. Typical result using serum on the RayBio® Autoimmune Disease IgG Autoantibody Detection Array. The protein arrays were incubated with diluent alone (A, B), diluted serum samples derived from normal individuals (C, D) and those from various autoimmune disease patients (E-L). Yellow boxes, show various controls as indicated (biotin-labeled BSA, human IgG and IgA).Storage/StabilityUpon receipt, all components of the RayBio® Human Protein Array kit should be stored at -20 °C to -80 °C until just before the experiment. At -20 °C to -80 °C the kit will retain complete activity for up to 6 months. Please use within six months of purchase. Once thawed, the array glass slide and Blocking Buffer should be kept at -20 °C and all other component should be stored at 4 °C. Use within three months after reagents have been thawed.No posts found Write A ReviewManualSDSGALAnalysis Tool