Antigen InformationGene Symbols:ABL1 | AXL | BLK | BMX | BTK | CSF1R | CSK | DDR2 | EGFR | EPHA1| EPHA2 | EPHA3 | EPHA4 | EPHA5 | EPHA6 | EPHA7 | EPHA8 | EPHB1 | EPHB2 | EPHB3 | EPHB4 | EPHB6 | ERBB2 | ERBB3 | ERBB4 | FER | FGFR1 | FGFR2 | FGR | FLT4 | FRK | FYN | HCK | IGF1R | INSR | ITK | JAK1 | JAK2 | JAK3 | KDR | KIT | LCK | LTK | LYN | MATK | MET | MUSK | NGFR | NTRK2 | PDGFRA | PDGFRB | PTK2 | PTK2B | RET | ROR1 | ROR2 | ROS1 | RYK | SLPI | SRMS | SYK | TEC | TEK | TIE1 | TNK1 | TNK2 | TXK | TYK2 | TYRO3 | ZAP70 View more HideAssay FormatPathway:PI3K-AKT SignalingNumber of Targets Detected:71Species Detected:HumanCompatible Sample Types:Cell Culture SupernatantsCell LysatesPlasmaSerumTissue LysatesDesign Principle:Sandwich-basedMethod of Detection:ChemiluminescenceQuantitative/Semi-Quantitative:Semi-QuantitativeSolid Support:MembraneProduct SpecificationsSize:2, 4, or 8 Sample KitProduct FeaturesEasy to useNo specialized equipment neededCompatible with nearly any liquid sampleProven technology (many publications)Highly sensitive (pg/ml)Sandwich ELISA specificityHigher density than ELISA, Western blot or bead-based multiplexTarget NamesScroll over each target protein for more informationABL1Gene Symbol: ABL1Gene ID: 25Accession #: P00519ACKGene Symbol: TNK2Gene ID: 10188Accession #: Q07912ELISA Kit: RayBio Human Phosphotyrosine ACK ELISAALK-1Gene Symbol: ALKGene ID: 238Accession #: Q9UM73ELISA Kit: RayBio Human Phosphotyrosine ALK ELISAAxlGene Symbol: AxlGene ID: 558Accession #: P30530ELISA Kit: RayBio Human Phosphotyrosine AXL ELISABlkGene Symbol: BLKGene ID: 640Accession #: P51451ELISA Kit: RayBio Human Phosphotyrosine BLK ELISABMXGene Symbol: BMXGene ID: 660Accession #: P51813ELISA Kit: RayBio Human Phosphotyrosine BMX ELISABtkGene Symbol: BTKGene ID: 695Accession #: Q06187ELISA Kit: RayBio Human Phosphotyrosine BTK ELISACskGene Symbol: CSKGene ID: 1445Accession #: P41240ELISA Kit: RayBio Human Phosphotyrosine CSK ELISADtkGene Symbol: TYRO3Gene ID: 7301Accession #: Q06418ELISA Kit: RayBio Human Phosphotyrosine DTK ELISAEGFRGene Symbol: EGFRGene ID: 1956Accession #: P00533ELISA Kit: RayBio Human Phosphotyrosine EGFR ELISAEphA1Gene Symbol: EphA1Gene ID: 2041Accession #: P21709ELISA Kit: RayBio Human Phosphotyrosine EphA1 ELISAEphA2Gene Symbol: EphA2Gene ID: 1969Accession #: P29317ELISA Kit: RayBio Human Phosphotyrosine EphA2 ELISAEphA3Gene Symbol: EphA3Gene ID: 2042Accession #: P29320ELISA Kit: RayBio Human Phosphotyrosine EphA3 ELISAEphA4Gene Symbol: EphA4Gene ID: 2043Accession #: P54764ELISA Kit: RayBio Human Phosphotyrosine EphA4 ELISAEphA5Gene Symbol: EphA5Gene ID: 2044Accession #: P54756ELISA Kit: RayBio Human Phosphotyrosine EphA5 ELISAEphA6Gene Symbol: EphA6Gene ID: 285220Accession #: Q9UF33ELISA Kit: RayBio Human Phosphotyrosine EphA6 ELISAEphA7Gene Symbol: EphA7Gene ID: 2045Accession #: Q15375ELISA Kit: RayBio Human Phosphotyrosine EphA7 ELISAEphA8Gene Symbol: EphA8Gene ID: 2046Accession #: P29322ELISA Kit: RayBio Human Phosphotyrosine EphA8 ELISAEphB1Gene Symbol: EphB1Gene ID: 2047Accession #: P54762ELISA Kit: RayBio Human Phosphotyrosine EphB1 ELISAEphB2Gene Symbol: EphB2Gene ID: 2048Accession #: P29323ELISA Kit: RayBio Human Phosphotyrosine EphB2 ELISAEphB3Gene Symbol: EphB3Gene ID: 2049Accession #: P54753ELISA Kit: RayBio Human Phosphotyrosine EphB3 ELISAEphB4Gene Symbol: EphB4Gene ID: 2050Accession #: P54760ELISA Kit: RayBio Human Phosphotyrosine EphB4 ELISAEphB6Gene Symbol: EphB6Gene ID: 2051Accession #: O15197ELISA Kit: RayBio Human Phosphotyrosine EphB6 ELISAErbB2Gene Symbol: ErbB2Gene ID: 2064Accession #: P04626ELISA Kit: RayBio Human Phosphotyrosine Erb-B2 ELISAErbB3Gene Symbol: ErbB3Gene ID: 2065Accession #: P21860ELISA Kit: RayBio Human Phosphotyrosine Erb-B3 ELISAErbB4Gene Symbol: ErbB4Gene ID: 2066Accession #: Q15303ELISA Kit: RayBio Human Phosphotyrosine Erb-B4 ELISAFAKGene Symbol: PTK2Gene ID: 5747Accession #: Q05397ELISA Kit: RayBio Human Phosphotyrosine FAK ELISAFERGene Symbol: FERGene ID: 2241Accession #: P16591ELISA Kit: RayBio Human Phosphotyrosine FER ELISAFGFR1Gene Symbol: FGFR1Gene ID: 2260Accession #: P11362ELISA Kit: RayBio Human Phosphotyrosine FGFR1 ELISAFGFR2Gene Symbol: FGFR2Gene ID: 2263Accession #: P21802ELISA Kit: RayBio Human Phosphotyrosine FGFR2 ELISAFGFR2 alpha isoformGene Symbol: FGFR2Gene ID: 2263Accession #: P21802FgrGene Symbol: FGRGene ID: 2268Accession #: P09769ELISA Kit: RayBio Human Phosphotyrosine FGR ELISAFRKGene Symbol: FRKGene ID: 2444Accession #: P42685ELISA Kit: RayBio Human Phosphotyrosine FRK ELISAFynGene Symbol: FYNGene ID: 2534Accession #: P06241ELISA Kit: RayBio Human Phosphotyrosine FYN ELISAHckGene Symbol: HCKGene ID: 3055Accession #: P08631ELISA Kit: RayBio Human Phosphotyrosine HCK ELISAHGFRGene Symbol: METGene ID: 4233Accession #: P08581ELISA Kit: RayBio Human HGF R ELISAIGF-1 RGene Symbol: IGF1RGene ID: 3480Accession #: P08069ELISA Kit: RayBio Human Phosphotyrosine IGF-1R ELISAInsulin R(CD220)Gene Symbol: INSRGene ID: 3643Accession #: P06213ELISA Kit: RayBio Human Phosphotyrosine Insulin R ELISAItkGene Symbol: ITKGene ID: 3702Accession #: Q08881ELISA Kit: RayBio Human Phosphotyrosine ITK ELISAJAK1Gene Symbol: JAK1Gene ID: 3716Accession #: P23458ELISA Kit: RayBio Human Phosphotyrosine JAK1 ELISAJAK2Gene Symbol: JAK2Gene ID: 3717Accession #: O60674ELISA Kit: RayBio Human Phosphotyrosine JAK2 ELISAJAK3Gene Symbol: JAK3Gene ID: 3718Accession #: P52333ELISA Kit: RayBio Human Phosphotyrosine JAK3 ELISALCKGene Symbol: LCKGene ID: 3932Accession #: P06239ELISA Kit: RayBio Human Phosphotyrosine LCK ELISALTKGene Symbol: LTKGene ID: 4058Accession #: P29376ELISA Kit: RayBio Human Phosphotyrosine LTK ELISALynGene Symbol: LYNGene ID: 4067Accession #: P07948ELISA Kit: RayBio Human Phosphotyrosine LYN ELISAMATKGene Symbol: MATKGene ID: 4145Accession #: P42679ELISA Kit: RayBio Human Phosphotyrosine MATK ELISAM-CSF RGene Symbol: CSF1RGene ID: 1436Accession #: P07333ELISA Kit: RayBio Human Phosphotyrosine MCSFR ELISAMUSKGene Symbol: MuSKGene ID: 4593Accession #: O15146ELISA Kit: RayBio Human Phosphotyrosine MUSK ELISANGFR(TNFRSF16)Gene Symbol: NGFRGene ID: 4804Accession #: P08138ELISA Kit: RayBio Human Phosphotyrosine NGFR ELISAPDGFRAGene Symbol: PDGFRAGene ID: 5156Accession #: P16234ELISA Kit: RayBio Human Phosphotyrosine PDGFRA ELISAPDGFRBGene Symbol: PDGFRBGene ID: 5159Accession #: P09619ELISA Kit: RayBio Human Phosphotyrosine PDGFRB ELISAPYK2Gene Symbol: PTK2BGene ID: 2185Accession #: Q14289ELISA Kit: RayBio Human Phosphotyrosine PYK2 ELISARETGene Symbol: RETGene ID: 5979Accession #: P07949ELISA Kit: RayBio Human Phosphotyrosine RET ELISAROR1Gene Symbol: ROR1Gene ID: 4919Accession #: Q01973ELISA Kit: RayBio Human Phosphotyrosine ROR1 ELISAROR2Gene Symbol: ROR2Gene ID: 4920Accession #: Q01974ELISA Kit: RayBio Human Phosphotyrosine ROR2 ELISAROSGene Symbol: ROS1Gene ID: 6098Accession #: P08922ELISA Kit: RayBio Human Phosphotyrosine ROS ELISARYKGene Symbol: RYKGene ID: 6259Accession #: P34925ELISA Kit: RayBio Human Phosphotyrosine RYK ELISASCF R(CD117/c-kit)Gene Symbol: KITGene ID: 3815Accession #: P10721ELISA Kit: RayBio Human Phosphotyrosine SCFR ELISASRMSGene Symbol: SRMSGene ID: 6725Accession #: Q9H3Y6ELISA Kit: RayBio Human Phosphotyrosine SRMS ELISASYKGene Symbol: SYKGene ID: 6850Accession #: P43405ELISA Kit: RayBio Human Phosphotyrosine SYK ELISATecGene Symbol: TECGene ID: 7006Accession #: P42680ELISA Kit: RayBio Human Phosphotyrosine TEC ELISATie-1Gene Symbol: TIE1Gene ID: 7075Accession #: P35590ELISA Kit: RayBio Human Phosphotyrosine TIE1 ELISATie-2Gene Symbol: TEKGene ID: 7010Accession #: Q02763ELISA Kit: RayBio Human Phosphotyrosine TIE2 ELISATNK1Gene Symbol: TNK1Gene ID: 8711Accession #: Q13470ELISA Kit: RayBio Human Phosphotyrosine TNK1 ELISATRKBGene Symbol: NTRK2Gene ID: 4915Accession #: Q16620ELISA Kit: RayBio Human Phosphotyrosine TRKB ELISATXKGene Symbol: TXKGene ID: 7294Accession #: P42681ELISA Kit: RayBio Human Phosphotyrosine TXK ELISATyk2Gene Symbol: TYK2Gene ID: 7297Accession #: P29597ELISA Kit: RayBio Human Phosphotyrosine TYK2 ELISATYRO10(DDR2/TKT)Gene Symbol: DDR2Gene ID: 4921Accession #: Q16832ELISA Kit: RayBio Human Phosphotyrosine TYRO10 ELISAVEGFR2Gene Symbol: KDRGene ID: 3791Accession #: P35968ELISA Kit: RayBio Human VEGF R2 ELISAVEGFR3Gene Symbol: FLT4Gene ID: 2324Accession #: P35916ELISA Kit: RayBio Human Phosphotyrosine VEGFR3 ELISAZAP70Gene Symbol: ZAP70Gene ID: 7535Accession #: P43403ELISA Kit: RayBio Human Phosphotyrosine ZAP70 ELISASpecies DetectedHumanApplication NotesSuggested ApplicationMultiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological ProcessKit ComponentsHuman RTK Phosphorylation Antibody Array C1 MembranesBlocking BufferWash Buffer 1Wash Buffer 2Biotinylated Anti-Phospho Tyrosine Detection AntibodyStreptavidin-Conjugated HRPDetection Buffer CDetection Buffer DLysis Buffer (1 x 5 ml)Protease Inhibitor CocktailPhosphatase Inhibitor Cocktail II8-Well Incubation TrayPlastic SheetsArray TemplatesManualOther Materials RequiredPipettors, pipet tips and other common lab consumablesOrbital shaker or oscillating rockerTissue Paper, blotting paper or chromatography paperAdhesive tape or Saran WrapDistilled or de-ionized waterA chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE"s ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.Protocol OutlineBlock membranesIncubate with SampleIncubate with Biotinylated Detection Antibody CocktailIncubate with HRP-Conjugated StreptavidinIncubate with Detection BuffersImage with chemiluminescent imaging systemPerform densitometry and analysisStorage/StabilityFor best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.Long, Zhi H., et al. "miR-141 inhibits proliferation and migration of colorectal cancer SW480 cells."?Anticancer research?37.8 (2017): 4345-4352.Species: HumanSample type: Cell LysatesWang Z., Gao Q., Shi J., et al. Protein tyrosine phosphatase receptor S acts as a metastatic suppressor in hepatocellular carcinoma by control of epithermal growth factor receptor-induced epithelial-mesenchymal transition. Hepatology. 2015 Oct;62(4):1201-14. doi: 10.1002/hep.27911. Species: HumanSample type: Cell LysateCortelazzi B., Verderio P., Ciniselli CM., et al. Receptor tyrosine kinase profiles and human papillomavirus status in oropharyngeal squamous cell carcinoma.J Oral Pathol Med. 2014 Dec 14. doi: 10.1111/jop.12301. Epub ahead of printSpecies: HumanSample type: Tissue LysateAcquired resistance to sunitinib in human renal cell carcinoma cells is mediated by constitutive activation of signal transduction pathways associated with tumour cell proliferationSpecies: HumanSample type: Conditioned MediaAl-aidaroos A., et al. Metastasis-associated PRL-3 induces EGFR activation and addiction in cancer cells. J Clin Invest. 2013;123(8):3459-3471. doi:10.1172/JCI66824. Species: HumanSample type: Cell LysateSiu IM., et al. Erlotinib Inhibits Growth of a Patient-Derived Chordoma Xenograft . Published: November 15, 2013 DOI: 10.1371/journal.pone.0078895Species: HumanSample type: Cell LysateGuerra-Laso, JosÃ? M., et al. "Macrophages from elders are more permissive to intracellular multiplication of Mycobacterium tuberculosis."?Age?35.4 (2013): 1235-1250. Species: HumanSample type: Cell LysateTirado-GonzÃ?lez, I., et al. "Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy."?Molecular human reproduction?19.1 (2013): 43.Species: HumanSample type: Cell LysateStrevens, Helena, et al. "PP038. Renal ETK/BMX activation decreased in preeclampsia."?Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health?3.2 (2013): 80.Species: HumanSample type: Tissue LysateKato K., Gong J., Iwama H., et al. The antidiabetic drug metformin inhibits gastric cancer cell proliferation in vitro and in vivo. Mol Cancer Ther. 2012 Mar;11(3):549-60. doi: 10.1158/1535-7163.MCT-11-0594Species: HumanSample type: Cell LysatePrev12NextKit worked pretty well. It would be nice if the rows and columns were labelled somehow. It was somewhat difficult to to determine which row was which if two rows next to each other had no RTKs with signal. Protocol was easy to follow.Matt SwiatnickiMichigan State University11/13/2019, 02:47 PMWrite A ReviewManualSDS