产品说明
OverviewTip Material: Stainless SteelFor use with NanoFil syringesPackage of 2Assortment packages are availableDetailsClick here to view the current NanoFil Data Sheet.OptionsOrder codeDescriptionGaugeBevel/BluntPkg SizeNF33BL-2Nanofil Needle33 GBlunt2NF34BL-2Nanofil Needle34 GBlunt2NF35BL-2Nanofil Needle35 GBlunt2NF36BL-2Nanofil Needle36 GBlunt2NF33BV-2Nanofil Needle33 GBeveled2NF34BV-2Nanofil Needle34 GBeveled2NF35BV-2Nanofil Needle35 GBeveled2NF36BV-2Nanofil Needle36 GBeveled2NF33-36BLNanofil Needle Assortment33-36 GBlunt4 (1 of each size)NF33-36BVNanofil Needle Assortment33-36 GBeveled4 (1 of each size)Selecting the Correct Tip for your ApplicationThe replaceable needles used with the NanoFil are available with either blunt or beveled tips. The blunt tip is used for injection into soft tissue and when a uniform solution distribution is needed. The beveled style is used for applications that involve the penetration of a tough tissue.One of the main factors that limit the resolution and accuracy of conventional micro syringes to the upper tens of nanoliters range is diffusion in the large tip ID. When the tip ID is equal or larger than 100μm, the error caused by tip diffusion is in the nanoliter range level [(100μm)3 = 1 nL]. With a 36 gauge needle installed on the NanoFil, the error caused by diffusion will be reduced to the sub-nanoliter level, making accurate injection of a nanoliter possible.All of WPI’s beveled tips have a unique 25º tri-surface bevel that is optimized for microinjection. A 10º single-surface beveled tip penetrates better than one with a 25º angle, however the distance between the upper opening to the tip (the dimension “F” in the drawing above) is longer. As a result, it requires a deeper penetration of the tip to achieve the same level of liquid delivery. Deeper penetration means more tissue damage. WPI’s unique 25º beveled tip solvesthis problem with two extra beveled surfaces. The tip of a single surface beveled tip is actually a blade instead of a point. It dulls very quickly. In contrast, the tri-surfaced tip has a real point. It not only penetrates much better but is also much more durable. Our tests show that our 33 gauge, 25º beveled tip penetrates easier and lasts longer than other manufacturers’ 33 gauge, 10º single beveled tips. With a 35 gauge tri-surface beveled tip, the resistance to the penetration becomes even less. Each of our tips undergo a penetration test before leaving the factory to guarantee the best results for our customers.Available Tips33 gauge: This tip is similar to Hamilton’s 7762 and 7803 series removable needles in both tip length and outer diameter. However, our beveled tip version is shorter, more durable, and penetrates better due to the special tri-surface grinding technique. In the past, 33 gauge tips were the smallest size sold by other manufacturers and were frequently cited in literature. However, our new 35 gauge tip is much better for injections involving small animals, especially mice. Compared with Hamilton’s 33 gauge, 10 degree beveled tip, our 35 gauge 25 degree beveled tip can reduce the depth of penetration by almost 80%. The distance between the tip and the upper rim of the opening (dimension F on the drawing) is 348 microns for the 33 gauge tip. The distance for our 35 gauge tip is only 230 microns. In addition, the smaller tip size significantly reduces the required penetration force. In nearly all applications, a 33 gauge tip can be replaced with our 35 gauge tip and produce better results.34 gauge: This is a transitional size between the 33 gauge and 35 gauge. If the 35 gauge is too weak and the 33 gauge is too large, this makes a good alternative.35 gauge: This was the most popular and preferred tip of most scientists during our field trial. The combination of its strength, length, durability, and clogging resistance creates a balance with very little compromising of the individual properties. It is much smaller than the 33 gauge tip offered by other manufacturers. It is only slightly larger than the 36 gauge tip but is much stronger and less likely to be clogged. Samples can be directly loaded with this tip. Its 5 mm length is sufficient enough for almost all injection applications in mice.36 gauge: This is the smallest tip that is commercially available. The tip is so small that it can be inserted into the opening of the 33 gauge needle tip. Because this is pushing the limits of what current technology can produce, there are some limitations to consider before using. Its thin diameter makes it necessary to limit its length to 2.5 to 3 mm in order to maintain a usable strength. Since the tip ID is in the 25 to 50 micron range, it is very easily clogged. Therefore, only well filtered solutions can be used. Depending on the viscosity of the sample, the user might also need to pre-load the syringe with a regular tip before switching to this tip for injection. We recommend using the 35 gauge tip instead of the 36 gauge unless it is absolutely necessary.ResourcesNanoFil Microsyringe for Nanoliter InjectionSpecificationsTip Order NumberGuageTip O.D. “A”Tip I.D. “B”Tip Length “C”Total Length “D”Shank O.D. “E”Bevel Length “F”Total Dead VolumeTip MaterialNF33BV-233210 μm115 μm10 mm40 mm460 μm≈348 µm0.416 µLStainless SteelNF34BV-234185 μm85 μm5 mm35 mm460 μm≈290 µm0.199 µLStainless SteelNF35BV-235135 μm55 μm5 mm35 mm460 μm≈204 µm0.435 µLStainless SteelNF36BV-236120 μm35 μm3 mm33 mm460 μm≈156 µm0.340 µLStainless SteelNF33BL-233210 µm115 µm10 mm40 mm460 µm≈00.416 µLStainless SteelNF34BL-234185 µm85 µm5 mm35 mm460 µm≈00.199 µLStainless SteelNF35BL-235135 µm55 µm5 mm35 mm460 µm≈00.435 µLStainless SteelNF36BL-236120 µm35 µm3 mm33 mm460 µm≈00.340 µLStainless SteelReferencesGoel M, Sienkiewicz AE, Picciani R, Wang J, Lee RK, Bhattacharya SK, "Cochlin, intraocular pressure regulation and mechanosensing." PLoS One. 2012;7(4):e34309. Epub 2012 Apr 4.Reviews
WPI (World Precision Instruments Inc.,)是研发生命科学与医学科研仪器的领跑者,1967年在美国耶鲁大学(YaleUniversity)成立,总部位于美国佛罗里达州萨拉索塔市(Sarasota,Florida)。
WPI公司产品面广,产品线长,由60 年代初期的神经电生理产品,逐渐延伸到90 年代的生物感应器、光谱检测系列产品,到今天21 世纪再拓展到心血管生理、肌肉生理学和器官生理学等领域的产品,供应实验室产品种类超过5000种,是美国、英国、德国、加拿大、澳大利亚、新西兰、法国等欧美生命科学领域主流实验室仪器设备的重要供应商。近年来,WPI公司全新的肌肉生理学产品、全球*的单细胞肌肉张力测量系统、创新的生物荧光光谱测量系统等革新产品不断上市,满足了专家学者们更完善、更高效、更精确的研究需求。
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