首页 > Biotium > Biotium/Mix-n-Stain Enzyme Antibody Labeling Kits/92300/10-20-ug-labeling

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Biotium/Mix-n-Stain Enzyme Antibody Labeling Kits/92300/10-20-ug-labeling

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92300

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Mix-n-Stain™enzymeantibodylabelingkitsarearevolutionaryantibodylabelingtechnologythatallowsyoutolabelupto100ugofyourantibodywithHRP,alkalinephosphatase,orglucoseoxidasewithminimalhands-ontimeandwithoutapurificationstep.ThelabelingproceduretoleratesmanycommonbuffercomponentsandantibodystABIlizers.Mix-n-Stain™enzymeantibodylabelingkitsarearevolutionaryantibodylabelingtechnologythatallowsyoutolabelupto100ugofyourantibodywithanenzymewithminimalhands-ontimeandnopurificationstep.Thelabelingproceduretoleratesmanycommonbuffercomponentsandantibodystabilizers.Formoreinformation,pleasedownloadtheproductinformationsheetsforthekits.Thiskitisavailablewiththefollowingenzymes:HRP(horserADIshperoxidase),glucoseoxidase,andalkalinephosphatase.1)HorseradishperoxidaseconjugatescanbeusedforWesternblot,ELISA,immunohistochemistryandotherstandardimmunoassayapplications. TheenzymelabelcanbevisualizedwithchromogenicsubstratessuchasDAB,ABTS,TMBandTMBUSinthepresenceofhydrogenperoxide.Label10-20ug,25-50ug,or50-100ugofantibodyinabout3hours.2)Glucoseoxidaseisanenzymewhichcatalysestheoxidationofglucosewiththereleaseofhydrogenperoxide.Label25-50ug,or50-100ugofantibodyin2hours.3)AlkalinephosphataseconjugatescanbeusedforELISA,immunohistochemistryandotherstandardimmunoassayapplications. TheenzymelabelcanbevisualizedwithchromogenicsubstratessuchasBCIPandPNPPaswellasfluorogenicsubstratesuchasMUP.Label25-50ug,or50-100ugofantibodyin2hours.ProductInformationProductdescriptionLabelingtimeCatalognumberReactionsizeProductprotocolMix-n-Stain™HRPAntibodyLabelingKit3.5hours9230010-20ugantibodyPI-923009230192302Mix-n-StainHRP9230125-50ugantibody9230250-100ugantibodyMix-n-Stain™AlkalinePhosphataseAntibodyLabelingKit2hours9231425-50ugantibodyPI-9231492315APMix-n-Stain9231550-100ugantibodyMix-n-Stain™GlucoseOxidaseAntibodyLabelingKit2hours9231225-50ugantibodyPI-9231292313GOxMix-n-Stain9231350-100ugantibodyReferencesn/a

★EB（溴化乙锭）：溴化乙锭是一种高度灵敏的荧光染色剂，用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号，可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。

★SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下，SYBR Green I发出微弱的荧光，但一旦与双链DNA结合后，荧光大大增强。因此，SYBR Green I的荧光信号强度与双链DNA的数量相关，可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm，发射波长最大约为520nm。